The investigation of the effect of quercetin on HT-29 and HCT-116 colon cancer cell lines through the expression of RIPK1, RIPK3, and MLKL genes

Alp Can Tuncer; Sevval Has; Haydar Bağış; Esra Bozgeyik

Makale detayları

The investigation of the effect of quercetin on HT-29 and HCT-116 colon cancer cell lines through the expression of RIPK1, RIPK3, and MLKL genes

Yazarlar:

Alp Can Tuncer | Sevval Has | Haydar Bağış | Esra Bozgeyik

Yayıncı:

Adıyaman University

Ana Başlıklar:

Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.182-187

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001305469

pISSN:

2458-9179

Anahtar kelimeler:

Özet:

 Aim: Quercetin is a compound with anti-cancer activity in many types of cancer. However, studies showing the effects of quercetin on the necroptosis pathway are limited, and therefore, the aim of this study was to determine the effect of quercetin on the necroptosis pathway. Materials and Methods: Colon cancer cells were cultured and effect of different concentrations of quercetin on cell viability was determined by MTT method. Afterwards, the expression level of RIPK1, RIPK3 and MLKL genes were analyzed to determine the effect of quercetin on necroptosis. Results: The active dose of quercetin was found to be 50 µM in HT-29 cells p0.0286, while 100 µM was found in HCT-116 cells p0.009. A significant increase in the expression level of necroptosis markers was detected in HT-29 cells treated with 50 and 100 µM quercetin. Conclusion: The results of this study showed that quercetin may be an active regulator of the necroptosis pathway.

 Aim: Quercetin is a compound with anti-cancer activity in many types of cancer. However, studies showing the effects of quercetin on the necroptosis pathway are limited, and therefore, the aim of this study was to determine the effect of quercetin on the necroptosis pathway. Materials and Methods: Colon cancer cells were cultured and effect of different concentrations of quercetin on cell viability was determined by MTT method. Afterwards, the expression level of RIPK1, RIPK3 and MLKL genes were analyzed to determine the effect of quercetin on necroptosis. Results: The active dose of quercetin was found to be 50 µM in HT-29 cells p0.0286, while 100 µM was found in HCT-116 cells p0.009. A significant increase in the expression level of necroptosis markers was detected in HT-29 cells treated with 50 and 100 µM quercetin. Conclusion: The results of this study showed that quercetin may be an active regulator of the necroptosis pathway.

 Aim: Quercetin is a compound with anti-cancer activity in many types of cancer. However, studies showing the effects of quercetin on the necroptosis pathway are limited, and therefore, the aim of this study was to determine the effect of quercetin on the necroptosis pathway. Materials and Methods: Colon cancer cells were cultured and effect of different concentrations of quercetin on cell viability was determined by MTT method. Afterwards, the expression level of RIPK1, RIPK3 and MLKL genes were analyzed to determine the effect of quercetin on necroptosis. Results: The active dose of quercetin was found to be 50 µM in HT-29 cells p0.0286, while 100 µM was found in HCT-116 cells p0.009. A significant increase in the expression level of necroptosis markers was detected in HT-29 cells treated with 50 and 100 µM quercetin. Conclusion: The results of this study showed that quercetin may be an active regulator of the necroptosis pathway.

 Amaç: Quercetin kolon kanseri dahil birçok kanser çeşidinde anti-kanser aktivite gösteren bir bileşiktir. Ancak, quercetinin nekroptoz yolağı üzerine etkilerini gösteren çalışmalar kısıtlıdır ve bu nedenle bu çalışmada quercetinin nekroptoz yolağına etkisinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: HT-29 ve HCT-116 kolon kanseri hücreleri kültür edilip farklı konsantrasyondaki quercetinin hücre canlılığına etkisi MTT yöntemi ile belirlendi. Sonrasında quercetinin nekroptoza etkisinin belirlenmesi için RIPK1, RIPK3 ve MLKL genlerinin ekspresyon seviyesi analiz edildi. Bulgular: HT-29 hücrelerinde quercetinin aktif dozu 50 µM p0,0286 olarak bulunurken HCT-116 hücrelerinde 100 µM p0,009 bulundu. 50 ve 100 µM quercetin ile maruz bırakılan HT-29 hücrelerinde nekroptoz belirteçlerinin ekspresyon seviyesinde ciddi bir artış tespit edildi. Sonuç: Bu çalışmanın sonuçları quercetinin nekroptoz yolağının aktif bir düzenleyicisi olabileceğini göstermiştir.

Makale detayları

The investigation of the effect of quercetin on HT-29 and HCT-116 colon cancer cell lines through the expression of RIPK1, RIPK3, and MLKL genes

Yazarlar:

Alp Can Tuncer | Sevval Has | Haydar Bağış | Esra Bozgeyik

Yayıncı:

Adıyaman University

Ana Başlıklar:

Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.182-187

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001305469

pISSN:

2458-9179

Colon cancer

MLKL

Necroptosis

Quercetin

RIPK1

RIPK3Kolon kanseri

MLKL

Nekroptoz

Quercetin

RIPK1

RIPK3

Sayı

Makale detayları

Yazarlar: Alp Can Tuncer | Sevval Has | Haydar Bağış | Esra Bozgeyik

Yayıncı: Adıyaman University

Ana Başlıklar: Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi: Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.182-187

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001305469

pISSN: 2458-9179

Sayı

Yazarlar:

Alp Can Tuncer | Sevval Has | Haydar Bağış | Esra Bozgeyik

Yayıncı:

Adıyaman University

Ana Başlıklar:

Kanser Hücre Biyolojisi | Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.9 Issue 3, pp.182-187

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001305469

pISSN:

2458-9179