Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities

Ali Savaş Bülbül; Muhammed Bekmezci; Fatih Şen

Makale detayları

Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities

Yazarlar:

Ali Savaş Bülbül | Muhammed Bekmezci | Fatih Şen

Yayıncı:

Kütahya Dumlupinar University

Ana Başlıklar:

Nanochemistry

Dergi:

Journal of Scientific Reports-A Issue 055, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299686

pISSN:

2687-6167

Anahtar kelimeler:

Özet:

 It is very important to use the green synthesis approach that uses living things and plants. Using the biogenic reduction technique, silver nanoparticles were synthesized from the R. aculeatus plant for this research. Scanning electron microscopy SEM, Fourier transform infrared spectroscopy, X-ray diffraction XRD, and UV-vis spectroscopy was used to characterize the produced AgNPs FT-IR. After the In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy SEM, Fourier transform infrared spectroscopy FT-IR, X-ray diffraction XRD and UV-vis spectroscopy UV-VIS. According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10-3 gmL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.

 It is very important to use the green synthesis approach that uses living things and plants. Using the biogenic reduction technique, silver nanoparticles were synthesized from the R. aculeatus plant for this research. Scanning electron microscopy SEM, Fourier transform infrared spectroscopy, X-ray diffraction XRD, and UV-vis spectroscopy was used to characterize the produced AgNPs FT-IR. After the In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy SEM, Fourier transform infrared spectroscopy FT-IR, X-ray diffraction XRD and UV-vis spectroscopy UV-VIS. According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10-3 gmL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.

 It is very important to use the green synthesis approach that uses living things and plants. Using the biogenic reduction technique, silver nanoparticles were synthesized from the R. aculeatus plant for this research. Scanning electron microscopy SEM, Fourier transform infrared spectroscopy, X-ray diffraction XRD, and UV-vis spectroscopy was used to characterize the produced AgNPs FT-IR. After the In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy SEM, Fourier transform infrared spectroscopy FT-IR, X-ray diffraction XRD and UV-vis spectroscopy UV-VIS. According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10-3 gmL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.

 It is very important to use the green synthesis approach that uses living things and plants. Using the biogenic reduction technique, silver nanoparticles were synthesized from the R. aculeatus plant for this research. Scanning electron microscopy SEM, Fourier transform infrared spectroscopy, X-ray diffraction XRD, and UV-vis spectroscopy was used to characterize the produced AgNPs FT-IR. After the In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy SEM, Fourier transform infrared spectroscopy FT-IR, X-ray diffraction XRD and UV-vis spectroscopy UV-VIS. According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10-3 gmL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.

Makale detayları

Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities

Yazarlar:

Ali Savaş Bülbül | Muhammed Bekmezci | Fatih Şen

Yayıncı:

Kütahya Dumlupinar University

Ana Başlıklar:

Nanochemistry

Dergi:

Journal of Scientific Reports-A Issue 055, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299686

pISSN:

2687-6167

Antimicrobial Activity

Antibiofilm Activity

Anticancer Activity

Biogenic Agent

Sayı

Makale detayları

Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities

Yazarlar: Ali Savaş Bülbül | Muhammed Bekmezci | Fatih Şen

Yayıncı: Kütahya Dumlupinar University

Ana Başlıklar: Nanochemistry

Dergi: Journal of Scientific Reports-A Issue 055, pp.1-15

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299686

pISSN: 2687-6167

Antimicrobial Activity

Antibiofilm Activity

Anticancer Activity

Biogenic Agent

Sayı

Yazarlar:

Ali Savaş Bülbül | Muhammed Bekmezci | Fatih Şen

Yayıncı:

Kütahya Dumlupinar University

Ana Başlıklar:

Nanochemistry

Dergi:

Journal of Scientific Reports-A Issue 055, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299686

pISSN:

2687-6167