Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Sevgi Altın; Cemalettin Alp; Ekrem Köksal; Sümeyye Akyüz

Makale detayları

Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Yazarlar:

Sevgi Altın | Cemalettin Alp | Ekrem Köksal | Sümeyye Akyüz

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 2, pp.1134-1141

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289849

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular. Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform OAC, ethanol OAE, and water OAW extract of Onobrychis argyrea subsp. argyrea O. argyrea specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71, A549 92.14, and HT-29 72.24 were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition IC50: 34.120.2 and 21.580.12, respectively. OAE 257.951.40 and OAW 282.140.83 extracts showed high activity with similar results in the FRAP method. Total phenolic content was determined as 226.151.89 mg GAEg for OAE and 146.520.71 mg GAEg for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich secondary metabolites.

 Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular. Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform OAC, ethanol OAE, and water OAW extract of Onobrychis argyrea subsp. argyrea O. argyrea specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71, A549 92.14, and HT-29 72.24 were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition IC50: 34.120.2 and 21.580.12, respectively. OAE 257.951.40 and OAW 282.140.83 extracts showed high activity with similar results in the FRAP method. Total phenolic content was determined as 226.151.89 mg GAEg for OAE and 146.520.71 mg GAEg for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich secondary metabolites.

 Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular. Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform OAC, ethanol OAE, and water OAW extract of Onobrychis argyrea subsp. argyrea O. argyrea specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71, A549 92.14, and HT-29 72.24 were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition IC50: 34.120.2 and 21.580.12, respectively. OAE 257.951.40 and OAW 282.140.83 extracts showed high activity with similar results in the FRAP method. Total phenolic content was determined as 226.151.89 mg GAEg for OAE and 146.520.71 mg GAEg for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich secondary metabolites.

 Fabaceae familyasına ait bitkiler, hayvan beslenmesinde ve insan sağlığının korunmasında antidiyabetik, antikanserojen, antioksidan, antienflamatuar ve kardiyovasküler büyük öneme sahiptir. Bu familyanın önemli cinslerinden biri olan Onobrychis yüksek fenolik içeriğe sahiptir ve güçlü bir antioksidan, antikanserojen ve antimikrobiyal ajan olarak bilinir. Bu çalışmada, Onobrychis argyrea subsp. argyrea O. argyrea türünün kloroform OAC, etanol OAE ve su OAW ekstreleri hazırlanmış ve bu ekstrelerin antiproliferatif, antimikrobiyal ve antioksidan aktiviteleri incelenmiştir. Ekstraktların antiproliferatif etkileri farklı kanser hücre dizileri üzerinde XTT kolorimetrik yöntemi ile değerlendirilmiştir. Bulgulara göre, OAE ekstresi tüm hücre hatlarına karşı en yüksek etkinliği göstermiştir. MCF-7 83.71, A549 92.14 ve HT-29 72.24 inhibe edildi. Tüm ekstrelerin Staphylococcus aureus, Escherichia coli ve Streptococcus pneumoniaeye karşı antimikrobiyal aktivitesi, disk difüzyon tekniği kullanılarak belirlendi. Son olarak antioksidan gücü DPPH ve FRAP yöntemleri kullanılarak belirlendi. DPPH yönteminde, OAE eksrtesi en yüksek radikal süpürme aktivitesi gösterdi IC50: 34.120.2. OAE 257.951.40 ve OAW 282.140.83 ekstreleri, FRAP yönteminde benzer sonuçlarda yüksek aktivite göstermiştir. Toplam fenolik madde içeriği OAE için 226.151.89 mg GAEg ve OAW için 146.520.71 mg GAEg olarak belirlendi. Bu sonuçlar, çalışılan bitkinin önemli bir antioksidan kaynağı olduğunu göstermektedir. Bütün bu sonuçlardan bitkinin çeşitli biyolojik aktivitelere ve zengin ikincil metabolitlere sahip olduğu anlaşılmaktadır.

Makale detayları

Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Yazarlar:

Sevgi Altın | Cemalettin Alp | Ekrem Köksal | Sümeyye Akyüz

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 2, pp.1134-1141

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289849

pISSN:

2536-4618

Onobrychis argyrea subsp. argyrea

antioxidant

antiproliferative

antimicrobialOnobrychis argyrea subsp. argyrea

antioksidan

antiproliferatif

antimikrobial

Sayı

Makale detayları

Yazarlar: Sevgi Altın | Cemalettin Alp | Ekrem Köksal | Sümeyye Akyüz

Yayıncı: Igdir University

Ana Başlıklar: Kimya Mühendisliği

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 2, pp.1134-1141

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001289849

pISSN: 2536-4618

Sayı

Yazarlar:

Sevgi Altın | Cemalettin Alp | Ekrem Köksal | Sümeyye Akyüz

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 2, pp.1134-1141

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289849

pISSN:

2536-4618