Development of a Highly Sensitive ELISA for Quantification of Hepatitis B Virus (HBV) Surface Antigen (HBsAg)

Resul KARAKUŞ; Latife Arzu ARAL; Bilkay BAŞTÜRK; Cemalettin AYBAY

Makale detayları

Development of a Highly Sensitive ELISA for Quantification of Hepatitis B Virus (HBV) Surface Antigen (HBsAg)

Yazarlar:

Resul KARAKUŞ | Latife Arzu ARAL | Bilkay BAŞTÜRK | Cemalettin AYBAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2007 Vol.37 Issue 2, pp.87-92

Belge Tipi:

Makale

Yayın Tarihi:

1/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000756043

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used.  Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes.  Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and Hep G2 were used for validation assays. Tests for human sera were run concurrently with the Access® Immunoassay System.  Results: The in-house ELISA system had an analytical sensitivity less than 0.41 ng/ml for both human sera and cell culture medium. The performance comparison of our in-house ELISA system with the Access® Immunoassay System even for samples with s/co levels of 1.5-13.7 gave a significant correlation.  Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.

 

Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used.  Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes.  Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and Hep G2 were used for validation assays. Tests for human sera were run concurrently with the Access® Immunoassay System.  Results: The in-house ELISA system had an analytical sensitivity less than 0.41 ng/ml for both human sera and cell culture medium. The performance comparison of our in-house ELISA system with the Access® Immunoassay System even for samples with s/co levels of 1.5-13.7 gave a significant correlation.  Conclusions: These reliable results of the newly established HBsAg ELISA system make it a promising candidate for diagnostic as well as research purposes.

Makale detayları

Development of a Highly Sensitive ELISA for Quantification of Hepatitis B Virus (HBV) Surface Antigen (HBsAg)

Yazarlar: Resul KARAKUŞ | Latife Arzu ARAL | Bilkay BAŞTÜRK | Cemalettin AYBAY

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2007 Vol.37 Issue 2, pp.87-92

Belge Tipi: Makale

Yayın Tarihi: 1/2007

Belge Dili: Türkçe

Document ID (Org No.): 2000756043

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

HBsAg

monoclonal antibody

ELISA

Sayı

Yazarlar:

Resul KARAKUŞ | Latife Arzu ARAL | Bilkay BAŞTÜRK | Cemalettin AYBAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2007 Vol.37 Issue 2, pp.87-92

Belge Tipi:

Makale

Yayın Tarihi:

1/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000756043

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144