A cost-effective alternative to standard medium enhances the pigment production and antibacterial activity of Spirulina

Muazzez Gürgan Eser; Çetin Yağcılar; Aslı Mutluç

Makale detayları

A cost-effective alternative to standard medium enhances the pigment production and antibacterial activity of Spirulina

Yazarlar:

Muazzez Gürgan Eser | Çetin Yağcılar | Aslı Mutluç

Yayıncı:

Kastamonu University

Ana Başlıklar:

Hidrobiyoloji | Gıda Bilimleri (Diğer)

Dergi:

MEMBA Su Bilimleri Dergisi Vol.11 Issue 2, pp.224-233

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352213

pISSN:

3062-3200

Anahtar kelimeler:

Özet:

 Spirulina Arthrospira platensis is a microalga with a high value due to the bioactive compounds it possesses. Spirulina is considered to be the super food and food of the future. It has many applications in various industries such as food, pharmaceuticals and dye industries. Replacing the standard growth medium for Spirulina with a more cost-effective alternative would offer a more sustainable production of microalgae. That is why in this study, there different growth media standard Zarrouks medium ZM, Low Medium LM and Modified Low medium MLM were tested in 5L bioreactors and bioactive compounds chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and phycocyanin in Spirulina were measured. Elemental analysis of the biomass was carried out by ICP-OES, and antibacterial activity of Spirulina extracts were analyzed. The results showed that the bioactive compound contents in Spirulina were elevated upon cultivation in MLM, which increased the antibacterial activity of the extracts. Lack of chelator in MLM significantly decreased the cost of the medium. Moreover, higher production of pigments suggested that there was no need to use high-cost micronutrient solution for the aim of pigment production, which definitely lowered the cost of medium. As a result, enhanced bioactive compound production on MLM compared to ZM and LM increases the economic value of the microalgae. Both reduced costs and increased bioactive compound contents make MLM a better choice for Spirulina production.

 Spirulina Arthrospira platensis is a microalga with a high value due to the bioactive compounds it possesses. Spirulina is considered to be the super food and food of the future. It has many applications in various industries such as food, pharmaceuticals and dye industries. Replacing the standard growth medium for Spirulina with a more cost-effective alternative would offer a more sustainable production of microalgae. That is why in this study, there different growth media standard Zarrouks medium ZM, Low Medium LM and Modified Low medium MLM were tested in 5L bioreactors and bioactive compounds chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and phycocyanin in Spirulina were measured. Elemental analysis of the biomass was carried out by ICP-OES, and antibacterial activity of Spirulina extracts were analyzed. The results showed that the bioactive compound contents in Spirulina were elevated upon cultivation in MLM, which increased the antibacterial activity of the extracts. Lack of chelator in MLM significantly decreased the cost of the medium. Moreover, higher production of pigments suggested that there was no need to use high-cost micronutrient solution for the aim of pigment production, which definitely lowered the cost of medium. As a result, enhanced bioactive compound production on MLM compared to ZM and LM increases the economic value of the microalgae. Both reduced costs and increased bioactive compound contents make MLM a better choice for Spirulina production.

 Spirulina Arthrospira platensis is a microalga with a high value due to the bioactive compounds it possesses. Spirulina is considered to be the super food and food of the future. It has many applications in various industries such as food, pharmaceuticals and dye industries. Replacing the standard growth medium for Spirulina with a more cost-effective alternative would offer a more sustainable production of microalgae. That is why in this study, there different growth media standard Zarrouks medium ZM, Low Medium LM and Modified Low medium MLM were tested in 5L bioreactors and bioactive compounds chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and phycocyanin in Spirulina were measured. Elemental analysis of the biomass was carried out by ICP-OES, and antibacterial activity of Spirulina extracts were analyzed. The results showed that the bioactive compound contents in Spirulina were elevated upon cultivation in MLM, which increased the antibacterial activity of the extracts. Lack of chelator in MLM significantly decreased the cost of the medium. Moreover, higher production of pigments suggested that there was no need to use high-cost micronutrient solution for the aim of pigment production, which definitely lowered the cost of medium. As a result, enhanced bioactive compound production on MLM compared to ZM and LM increases the economic value of the microalgae. Both reduced costs and increased bioactive compound contents make MLM a better choice for Spirulina production.

 Spirulina Arthrospira platensis, sahip olduğu biyoaktif bileşikler nedeniyle yüksek katma değere sahip bir mikro algdir. Spirulina süper gıda ve geleceğin gıdası olarak kabul edilmektedir. Gıda, ilaç ve boya endüstrileri gibi çeşitli sektörlerde birçok uygulama alanına sahiptir. Spirulina için standart büyüme ortamının daha uygun maliyetli bir alternatifle değiştirilmesi, daha sürdürülebilir bir mikroalg üretimi sağlayacaktır. Bu nedenle bu çalışmada, farklı büyüme ortamları standart Zarrouk ortamı ZM, Low Medium LM ve Modified Low medium MLM 5L biyoreaktörlerde test edilmiş ve Spirulinadaki biyoaktif bileşikler klorofil a, klorofil b, toplam klorofil, karotenoid ve fikosiyanin ölçülmüştür. Biyokütlenin element analizi ICP-OES ile gerçekleştirilmiş ve Spirulina ekstraktlarının antibakteriyel aktivitesi analiz edilmiştir. Sonuçlar, Spirulinadaki biyoaktif bileşik içeriklerinin MLMde yetiştirildiğinde arttığını ve bunun da ekstraktların antibakteriyel aktivitesini artırdığını göstermiştir. MLMde şelatör bulunmaması ortamın maliyetini önemli ölçüde azaltmıştır. Ayrıca, daha yüksek pigment üretimi, pigment üretimi amacıyla yüksek maliyetli mikro besin çözeltisi kullanılmasına gerek olmadığını göstermiş ve bu da ortam maliyetini ciddi anlamda düşürmüştür. Sonuç olarak, MLMde ZM ve LMye kıyasla daha fazla biyoaktif bileşik üretimi mikroalglerin ekonomik değerini artırmaktadır. Hem azalan maliyetler hem de artan biyoaktif bileşik içerikleri MLMyi Spirulina üretimi için daha iyi bir seçim haline getirmektedir.

Makale detayları

A cost-effective alternative to standard medium enhances the pigment production and antibacterial activity of Spirulina

Yazarlar:

Muazzez Gürgan Eser | Çetin Yağcılar | Aslı Mutluç

Yayıncı:

Kastamonu University

Ana Başlıklar:

Hidrobiyoloji | Gıda Bilimleri (Diğer)

Dergi:

MEMBA Su Bilimleri Dergisi Vol.11 Issue 2, pp.224-233

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352213

pISSN:

3062-3200

Arthrospira platensis

growth medium

phycocyanin

carotenoid

chlorophyll

antibacterial activityArthrospira platensis

growth medium

phycocyanin

carotenoid

chlorophyll

antibacterial activity

Sayı

Makale detayları

Yazarlar: Muazzez Gürgan Eser | Çetin Yağcılar | Aslı Mutluç

Yayıncı: Kastamonu University

Ana Başlıklar: Hidrobiyoloji | Gıda Bilimleri (Diğer)

Dergi: MEMBA Su Bilimleri Dergisi Vol.11 Issue 2, pp.224-233

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001352213

pISSN: 3062-3200

Sayı

Yazarlar:

Muazzez Gürgan Eser | Çetin Yağcılar | Aslı Mutluç

Yayıncı:

Kastamonu University

Ana Başlıklar:

Hidrobiyoloji | Gıda Bilimleri (Diğer)

Dergi:

MEMBA Su Bilimleri Dergisi Vol.11 Issue 2, pp.224-233

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352213

pISSN:

3062-3200