Assessment of genotoxic and cytotoxic effects in COVID-19 patients

Işıl Deniz Alıravcı; Yusuf Haydar Ertekin; Nihan Akıncı Kenanoğlu; Ahmet Ali Berber

Makale detayları

Assessment of genotoxic and cytotoxic effects in COVID-19 patients

Yazarlar:

Işıl Deniz Alıravcı | Yusuf Haydar Ertekin | Nihan Akıncı Kenanoğlu | Ahmet Ali Berber

Yayıncı:

Cukurova University

Ana Başlıklar:

Bulaşıcı Hastalıklar

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.11-21

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343848

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: The objective of our study was to ascertain whether the SARS-CoV-2 virus exerts direct cytotoxic and genotoxic effects on human blood defence cells. Materials and Methods: An in vitro analysis was conducted to assess the cytotoxic and genotoxic effects of the virus using three established tests: the mitotic index MI, micronucleus MN, and comet assay CA. These tests were applied to blood samples from 101 patients. The blood samples were simultaneously analyzed using the polymerase chain reaction PCR test. The study population included patients of all ages and genders who presented to the outpatient clinic with symptoms suggestive of a respiratory tract infection and fever. Results: The frequency of MN in the human lymphocytes of COVID-19-infected patients 1.06 was higher compared to COVID-19-negative patients 0.68. Similarly, in COVID-19-positive individuals, parameters such as tail length 3.67, tail moment 1.786, and tail intensity in the comet assay showed a significant increase compared to the negative control, indicating DNA damage. In the cytotoxicity assessment, the MI frequency of COVID-19-positive individuals 0.041 was significantly lower than that of negative controls 0.051. Gender did not influence the cytogenotoxicity except for tail length in SARS-CoV-2-infected patients. Among age groups, the SARS-CoV-2 virus increased MI frequency and tail intensity only in middle-aged individuals 2636 years. Conclusion: The SARS-CoV-2 virus has the potential to induce cytotoxic and genotoxic effects in the human lymphocytes of infected individuals.

 Purpose: The objective of our study was to ascertain whether the SARS-CoV-2 virus exerts direct cytotoxic and genotoxic effects on human blood defence cells. Materials and Methods: An in vitro analysis was conducted to assess the cytotoxic and genotoxic effects of the virus using three established tests: the mitotic index MI, micronucleus MN, and comet assay CA. These tests were applied to blood samples from 101 patients. The blood samples were simultaneously analyzed using the polymerase chain reaction PCR test. The study population included patients of all ages and genders who presented to the outpatient clinic with symptoms suggestive of a respiratory tract infection and fever. Results: The frequency of MN in the human lymphocytes of COVID-19-infected patients 1.06 was higher compared to COVID-19-negative patients 0.68. Similarly, in COVID-19-positive individuals, parameters such as tail length 3.67, tail moment 1.786, and tail intensity in the comet assay showed a significant increase compared to the negative control, indicating DNA damage. In the cytotoxicity assessment, the MI frequency of COVID-19-positive individuals 0.041 was significantly lower than that of negative controls 0.051. Gender did not influence the cytogenotoxicity except for tail length in SARS-CoV-2-infected patients. Among age groups, the SARS-CoV-2 virus increased MI frequency and tail intensity only in middle-aged individuals 2636 years. Conclusion: The SARS-CoV-2 virus has the potential to induce cytotoxic and genotoxic effects in the human lymphocytes of infected individuals.

 Purpose: The objective of our study was to ascertain whether the SARS-CoV-2 virus exerts direct cytotoxic and genotoxic effects on human blood defence cells. Materials and Methods: An in vitro analysis was conducted to assess the cytotoxic and genotoxic effects of the virus using three established tests: the mitotic index MI, micronucleus MN, and comet assay CA. These tests were applied to blood samples from 101 patients. The blood samples were simultaneously analyzed using the polymerase chain reaction PCR test. The study population included patients of all ages and genders who presented to the outpatient clinic with symptoms suggestive of a respiratory tract infection and fever. Results: The frequency of MN in the human lymphocytes of COVID-19-infected patients 1.06 was higher compared to COVID-19-negative patients 0.68. Similarly, in COVID-19-positive individuals, parameters such as tail length 3.67, tail moment 1.786, and tail intensity in the comet assay showed a significant increase compared to the negative control, indicating DNA damage. In the cytotoxicity assessment, the MI frequency of COVID-19-positive individuals 0.041 was significantly lower than that of negative controls 0.051. Gender did not influence the cytogenotoxicity except for tail length in SARS-CoV-2-infected patients. Among age groups, the SARS-CoV-2 virus increased MI frequency and tail intensity only in middle-aged individuals 2636 years. Conclusion: The SARS-CoV-2 virus has the potential to induce cytotoxic and genotoxic effects in the human lymphocytes of infected individuals.

 Amaç: Çalışmamızda COVID-19 virüsünün insan lenfositleri üzerindeki doğrudan sitotoksik ve genotoksik etkisinin olup olmadığının saptanması amaçlanmıştır. Gereç ve Yöntem:Virüsün sitotoksikgenotoksik etkileri 101 hastadan alınan kan örnekleri ile in vitro mitotik indeks MI, Mikronükleus MN ve Comet Assay SCGE testleri kullanılarak değerlendirilmiştir. Hastalardan alınan kan örnekleri PCR testi ile eş zamanlı olarak analiz edilmiştir. Çalışmaya COVID-19 polikliniğine üst solunum yolu ve ateş şikayetleriyle başvuran her yaş grubundan ve cinsiyetten hastalar dahil edilmiştir. Bulgular: COVID-19 ile enfekte olmuş hastaların insan lenfositlerindeki MN sıklığı 1.06 COVID-19 negatif hastalara 0.68 kıyasla artmıştır. Benzer şekilde COVID-19 pozitif bireylerde komet testindeki kuyruk uzunluğu 3.67, kuyruk momenti 1.786 ve kuyruk yoğunluğu parametreleri negative kontrole kıyasla önemli ölçüde artış göstererek DNA hasarına neden olmuşlardır. Sitotoksisite değerlendirmesinde COVID-19 pozitif bireylerin MI frekansı 0.041 negatiflere 0.051 göre anlamlı derecede düşmüştür. Cinsiyet SARS-CoV-2 enfekte hastalarda sitogenotoksisiteyi kuyruk uzunluğu hariç etkilememiştir. Yaş gruplarında, SARS-CoV-2 virüsü MI sıklığını ve kuyruk yoğunluğunu yalnızca orta yaşta 26-36 artırmıştır. Sonuç: SARS-CoV-2 virüsü, COVID-19 ile enfekte hastalarda insan lenfositleri üzerinde sitotoksik ve genotoksik etkilere neden olabilir.

Makale detayları

Assessment of genotoxic and cytotoxic effects in COVID-19 patients

Yazarlar:

Işıl Deniz Alıravcı | Yusuf Haydar Ertekin | Nihan Akıncı Kenanoğlu | Ahmet Ali Berber

Yayıncı:

Cukurova University

Ana Başlıklar:

Bulaşıcı Hastalıklar

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.11-21

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343848

pISSN:

2602-3032

Comet assay

COVID-19

genotoxicity

micronucleus

mitotic indexComet assay

COVID-19

genotoksisite

mikronükleus

mitotik indeks

Sayı

Makale detayları

Yazarlar: Işıl Deniz Alıravcı | Yusuf Haydar Ertekin | Nihan Akıncı Kenanoğlu | Ahmet Ali Berber

Yayıncı: Cukurova University

Ana Başlıklar: Bulaşıcı Hastalıklar

Dergi: Cukurova Medical Journal Vol.50 Issue 1, pp.11-21

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343848

pISSN: 2602-3032

Sayı

Yazarlar:

Işıl Deniz Alıravcı | Yusuf Haydar Ertekin | Nihan Akıncı Kenanoğlu | Ahmet Ali Berber

Yayıncı:

Cukurova University

Ana Başlıklar:

Bulaşıcı Hastalıklar

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.11-21

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343848

pISSN:

2602-3032