Chlorogenic acid nanoemulsion for Staphylococcus aureus causing skin infection: Synthesis, characterization and evaluation of antibacterial efficacy

Yasemin Budama-kilinc

Makale detayları

Chlorogenic acid nanoemulsion for Staphylococcus aureus causing skin infection: Synthesis, characterization and evaluation of antibacterial efficacy

Yazarlar:

Yasemin Budama-kilinc

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Computer Software

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.41 Issue 2, pp.322-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001311504

pISSN:

1304-7191

Anahtar kelimeler:

Özet:

 Staphylococcus aureus S. aureus causes many skin infections such as impetigo, infected abra-sions, cellulitis, folliculitis, subcutaneous abscesses, infected ulcers and sores. In this study, it was aimed to develop a nanoformulation of chlorogenic acid that was efficient against S. aureus. In this context, ultrasonic emulsification method was used for production of the chlo-rogenic acid CA nanoemulsion formulation and was characterized in detail. In addition, the kinetic and thermodynamic stability of the CA nanoemulsion formulation was examined. Finally, the broth microdilution method was used to determine the antibacterial activity of the formulation. It was determined that the average droplet size of the CA nanoemulsion for-mulation was 120.4  6.39 nm, the polydispersity index PdI was 0.180  0.018, and the zeta potential value was -11.5 1.15 mV. As a result of the thermodynamic stress tests of the CA na-noemulsion formulation, it was observed that there was no precipitation or phase separation. Moreover, in vitro release study showed a CA release of 75.49 after 48 hours. The antibac-terial results revealed that the CA nanoemulsion formulation was efficient 95 inhibition against S. aureus ATCC 25923. As a result, it is thought that CA nanoemulsion is an effective, nano sized and controlled release system based formulation candidate that may be used in the topical treatment of S. aureus causing skin infection.

 Staphylococcus aureus S. aureus causes many skin infections such as impetigo, infected abra-sions, cellulitis, folliculitis, subcutaneous abscesses, infected ulcers and sores. In this study, it was aimed to develop a nanoformulation of chlorogenic acid that was efficient against S. aureus. In this context, ultrasonic emulsification method was used for production of the chlo-rogenic acid CA nanoemulsion formulation and was characterized in detail. In addition, the kinetic and thermodynamic stability of the CA nanoemulsion formulation was examined. Finally, the broth microdilution method was used to determine the antibacterial activity of the formulation. It was determined that the average droplet size of the CA nanoemulsion for-mulation was 120.4  6.39 nm, the polydispersity index PdI was 0.180  0.018, and the zeta potential value was -11.5 1.15 mV. As a result of the thermodynamic stress tests of the CA na-noemulsion formulation, it was observed that there was no precipitation or phase separation. Moreover, in vitro release study showed a CA release of 75.49 after 48 hours. The antibac-terial results revealed that the CA nanoemulsion formulation was efficient 95 inhibition against S. aureus ATCC 25923. As a result, it is thought that CA nanoemulsion is an effective, nano sized and controlled release system based formulation candidate that may be used in the topical treatment of S. aureus causing skin infection.

 Staphylococcus aureus S. aureus causes many skin infections such as impetigo, infected abra-sions, cellulitis, folliculitis, subcutaneous abscesses, infected ulcers and sores. In this study, it was aimed to develop a nanoformulation of chlorogenic acid that was efficient against S. aureus. In this context, ultrasonic emulsification method was used for production of the chlo-rogenic acid CA nanoemulsion formulation and was characterized in detail. In addition, the kinetic and thermodynamic stability of the CA nanoemulsion formulation was examined. Finally, the broth microdilution method was used to determine the antibacterial activity of the formulation. It was determined that the average droplet size of the CA nanoemulsion for-mulation was 120.4  6.39 nm, the polydispersity index PdI was 0.180  0.018, and the zeta potential value was -11.5 1.15 mV. As a result of the thermodynamic stress tests of the CA na-noemulsion formulation, it was observed that there was no precipitation or phase separation. Moreover, in vitro release study showed a CA release of 75.49 after 48 hours. The antibac-terial results revealed that the CA nanoemulsion formulation was efficient 95 inhibition against S. aureus ATCC 25923. As a result, it is thought that CA nanoemulsion is an effective, nano sized and controlled release system based formulation candidate that may be used in the topical treatment of S. aureus causing skin infection.

 Staphylococcus aureus S. aureus causes many skin infections such as impetigo, infected abra-sions, cellulitis, folliculitis, subcutaneous abscesses, infected ulcers and sores. In this study, it was aimed to develop a nanoformulation of chlorogenic acid that was efficient against S. aureus. In this context, ultrasonic emulsification method was used for production of the chlo-rogenic acid CA nanoemulsion formulation and was characterized in detail. In addition, the kinetic and thermodynamic stability of the CA nanoemulsion formulation was examined. Finally, the broth microdilution method was used to determine the antibacterial activity of the formulation. It was determined that the average droplet size of the CA nanoemulsion for-mulation was 120.4  6.39 nm, the polydispersity index PdI was 0.180  0.018, and the zeta potential value was -11.5 1.15 mV. As a result of the thermodynamic stress tests of the CA na-noemulsion formulation, it was observed that there was no precipitation or phase separation. Moreover, in vitro release study showed a CA release of 75.49 after 48 hours. The antibac-terial results revealed that the CA nanoemulsion formulation was efficient 95 inhibition against S. aureus ATCC 25923. As a result, it is thought that CA nanoemulsion is an effective, nano sized and controlled release system based formulation candidate that may be used in the topical treatment of S. aureus causing skin infection.

Makale detayları

Chlorogenic acid nanoemulsion for Staphylococcus aureus causing skin infection: Synthesis, characterization and evaluation of antibacterial efficacy

Yazarlar:

Yasemin Budama-kilinc

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Computer Software

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.41 Issue 2, pp.322-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001311504

pISSN:

1304-7191

Nanoemulsion

Skin Infection

Chlorogenic Acid

Staphylococcus Aureus

Sayı

Makale detayları

Chlorogenic acid nanoemulsion for Staphylococcus aureus causing skin infection: Synthesis, characterization and evaluation of antibacterial efficacy

Yazarlar: Yasemin Budama-kilinc

Yayıncı: Yildiz Technical University

Ana Başlıklar: Computer Software

Dergi: Sigma Journal of Engineering and Natural Sciences Vol.41 Issue 2, pp.322-330

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001311504

pISSN: 1304-7191

Nanoemulsion

Skin Infection

Chlorogenic Acid

Staphylococcus Aureus

Sayı

Yazarlar:

Yasemin Budama-kilinc

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Computer Software

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.41 Issue 2, pp.322-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001311504

pISSN:

1304-7191