An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (Linnaeus, 1758) from Turkish coasts

Gökmen Zafer Pekmezci

Makale detayları

An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (Linnaeus, 1758) from Turkish coasts

Yazarlar:

Gökmen Zafer Pekmezci

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other) | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.245-251

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292301

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 The diplectanid monogenean Diplectanum aequans D. aequans Wegener, 1857 is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax Dic. labrax Linnaeus, 1758 worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea FAO area 37.4.2 and Aegean Sea FAO area 37.1.3 coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100 and 15.1, respectively. This study reports first information of molecular 28S and 18S rRNA evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.

 The diplectanid monogenean Diplectanum aequans D. aequans Wegener, 1857 is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax Dic. labrax Linnaeus, 1758 worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea FAO area 37.4.2 and Aegean Sea FAO area 37.1.3 coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100 and 15.1, respectively. This study reports first information of molecular 28S and 18S rRNA evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.

 The diplectanid monogenean Diplectanum aequans D. aequans Wegener, 1857 is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax Dic. labrax Linnaeus, 1758 worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea FAO area 37.4.2 and Aegean Sea FAO area 37.1.3 coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100 and 15.1, respectively. This study reports first information of molecular 28S and 18S rRNA evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.

 Monogeneanlar deniz balıklarının yaygın ektoparazitik yassı kurtlarıdır. Diplectanid monogenean Diplectanum aequans Wegener, 1857 Diplectanidae dünya çapında doğal ve kültüre edilen Avrupa deniz levreği Dicentrarchus labrax Linnaeus, 1758 türünün en önemli ektoparazitlerden biridir. Bu parazit türü Avrupa deniz levreklerinin solungaçlarında ciddi patolojik hasarlara ve enfekte levreklerde ölüme neden olur. Halen Türkiye kıyılarından D. aequansın moleküler verileri hakkında bilgi bulunmamaktadır. Bu çalışmada Türkiyenin Karadeniz FAO 37.4.2 ve Ege Denizi FAO 37.1.3 kıyılarındaki doğal ve kültüre edilen D. labrax L.dan diplectanid monogeneanlar toplandı, morfolojik olarak identifiye edildi ve 28S rRNA ile 18S rRNA parçalarının DNA dizilenmesiyle genetik olarak karakterize edildi. Toplanan diplectanidler ayrıntılı morfolojik özelliklerine ve 28S rRNA ile 18S rRNA genlerinin dizi karakterizasyonuna bağlı olarak açık bir şekilde D. aequans olarak teşhis edildi. Diplectanum aequansın enfeksiyon oranı ve ortalama yoğunluğu sırasıyla 100 ve 15,1 idi. Bu çalışma Türkiye kıyılarından örneklenen D. labraxtan izole edilen D. aequansın moleküler 28S ve 18S rRNA kanıtlarına ilişkin ilk bilgileri sunmaktadır. Karadeniz FAO 37.4.2 ve Ege Denizinden FAO 37.1.3 izole edilen D. aequans için yeni 18S ve 28S rRNA dizileri genetik olarak karakterize edilmiştir. Diplectanum aequansın 28S ve 18S rRNA dizileri Karadeniz ve Akdenizdeki Diplectanidae türlerinin filogenetik pozisyonlarını çözmek için kullanılabilir.

Makale detayları

An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (Linnaeus, 1758) from Turkish coasts

Yazarlar:

Gökmen Zafer Pekmezci

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other) | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.245-251

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292301

pISSN:

1300-0861

Diplectanidae

DNA sequencing

Phylogenetic analysis

18S rRNA

28S rRNADiplectanidae

DNA dizi analizi

Filogenetik analiz

18S rRNA

28S rRNA

Sayı

Makale detayları

Yazarlar: Gökmen Zafer Pekmezci

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Sciences (Other) | Veterinary Surgery

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.245-251

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001292301

pISSN: 1300-0861

Sayı

Yazarlar:

Gökmen Zafer Pekmezci

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Sciences (Other) | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.245-251

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292301

pISSN:

1300-0861