Celtis australis Exhibits Cytotoxic Effects by Inducing DNA Damage in Human Ovarian Cancer Cells

Gülsüm Nur Fidan; Sevilay Günay; Yavuz Erden

Makale detayları

Celtis australis Exhibits Cytotoxic Effects by Inducing DNA Damage in Human Ovarian Cancer Cells

Yazarlar:

Gülsüm Nur Fidan | Sevilay Günay | Yavuz Erden

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 1, pp.7-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314168

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Native to the Mediterranean and southwest Asia, Celtis australis is used in folk medicine to treatment of many diseases such as peptic ulcer, diarrhea, dysentery, pain, and colic. However, there are very few studies on the effects of this species on cancer. We report for the first time the cytotoxic and genotoxic properties of C. australis on the human ovarian cancer cell line A2780. In the study, C. australis extract prepared in ethanolphosphate buffer was applied to A2780 cells. The change in viability level in A2780 cells after treatment was determined by MTT assay and DNA damage was determined by single-cell gel electrophoresis Comet analysis. Our results showed that plant extract application at doses above 200 µgml significantly decreased A2780 cell viability p0.05. The IC50 value for A2780 cells of C. australis was determined as 251.43 μgml. Comet analysis results revealed that 50 µgml and above dose applications increased the level of DNA damage p0.05. These results suggest that C. australis mediates cell death by inducing DNA damage in A2780 cancer cells.

 Native to the Mediterranean and southwest Asia, Celtis australis is used in folk medicine to treatment of many diseases such as peptic ulcer, diarrhea, dysentery, pain, and colic. However, there are very few studies on the effects of this species on cancer. We report for the first time the cytotoxic and genotoxic properties of C. australis on the human ovarian cancer cell line A2780. In the study, C. australis extract prepared in ethanolphosphate buffer was applied to A2780 cells. The change in viability level in A2780 cells after treatment was determined by MTT assay and DNA damage was determined by single-cell gel electrophoresis Comet analysis. Our results showed that plant extract application at doses above 200 µgml significantly decreased A2780 cell viability p0.05. The IC50 value for A2780 cells of C. australis was determined as 251.43 μgml. Comet analysis results revealed that 50 µgml and above dose applications increased the level of DNA damage p0.05. These results suggest that C. australis mediates cell death by inducing DNA damage in A2780 cancer cells.

 Native to the Mediterranean and southwest Asia, Celtis australis is used in folk medicine to treatment of many diseases such as peptic ulcer, diarrhea, dysentery, pain, and colic. However, there are very few studies on the effects of this species on cancer. We report for the first time the cytotoxic and genotoxic properties of C. australis on the human ovarian cancer cell line A2780. In the study, C. australis extract prepared in ethanolphosphate buffer was applied to A2780 cells. The change in viability level in A2780 cells after treatment was determined by MTT assay and DNA damage was determined by single-cell gel electrophoresis Comet analysis. Our results showed that plant extract application at doses above 200 µgml significantly decreased A2780 cell viability p0.05. The IC50 value for A2780 cells of C. australis was determined as 251.43 μgml. Comet analysis results revealed that 50 µgml and above dose applications increased the level of DNA damage p0.05. These results suggest that C. australis mediates cell death by inducing DNA damage in A2780 cancer cells.

 Native to the Mediterranean and southwest Asia, Celtis australis is used in folk medicine to treatment of many diseases such as peptic ulcer, diarrhea, dysentery, pain, and colic. However, there are very few studies on the effects of this species on cancer. We report for the first time the cytotoxic and genotoxic properties of C. australis on the human ovarian cancer cell line A2780. In the study, C. australis extract prepared in ethanolphosphate buffer was applied to A2780 cells. The change in viability level in A2780 cells after treatment was determined by MTT assay and DNA damage was determined by single-cell gel electrophoresis Comet analysis. Our results showed that plant extract application at doses above 200 µgml significantly decreased A2780 cell viability p0.05. The IC50 value for A2780 cells of C. australis was determined as 251.43 μgml. Comet analysis results revealed that 50 µgml and above dose applications increased the level of DNA damage p0.05. These results suggest that C. australis mediates cell death by inducing DNA damage in A2780 cancer cells.

Makale detayları

Celtis australis Exhibits Cytotoxic Effects by Inducing DNA Damage in Human Ovarian Cancer Cells

Yazarlar:

Gülsüm Nur Fidan | Sevilay Günay | Yavuz Erden

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 1, pp.7-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314168

pISSN:

2149-6366

Ovarian cancer

Celtis Australis

Cytotoxicity

DNA Damage

Sayı

Makale detayları

Celtis australis Exhibits Cytotoxic Effects by Inducing DNA Damage in Human Ovarian Cancer Cells

Yazarlar: Gülsüm Nur Fidan | Sevilay Günay | Yavuz Erden

Yayıncı: Bingol University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi: Türk Doğa ve Fen Dergisi Vol.13 Issue 1, pp.7-11

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314168

pISSN: 2149-6366

Ovarian cancer

Celtis Australis

Cytotoxicity

DNA Damage

Sayı

Yazarlar:

Gülsüm Nur Fidan | Sevilay Günay | Yavuz Erden

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 1, pp.7-11

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314168

pISSN:

2149-6366