Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis

Gülçin BÖLÜK; Esra KAZAK; Fahir ÖZKALEMKAŞ; Beyza ENER; Halis AKALIN; Harun AĞCA; Yildiz OKUTURLAR; Kürşad KESKİN; Başak BURGAZLIOĞLU; Ridvan ALİ

Makale detayları

Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis

Yazarlar:

Gülçin BÖLÜK | Esra KAZAK | Fahir ÖZKALEMKAŞ | Beyza ENER | Halis AKALIN | Harun AĞCA | Yildiz OKUTURLAR | Kürşad KESKİN | Başak BURGAZLIOĞLU | Ridvan ALİ

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2016 Vol.46 Issue 2, pp.335-342

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000752181

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Bckground/aim: Invasive aspergillosis (IA) is a fatal infection that is difficult to diagnose in immunocompromised patients. In thisstudy, Aspergillus-specific DNA was searched using real-time PCR (RT-PCR) in serum samples. Galactomannan (GM) and/or beta-Dglucan (BDG) tests were previously performed on these samples for 70 neutropenic patients with hematological malignancy.Materials and methods: The patients were categorized according to the criteria of the European Organization for Research andTreatment of Cancer/Mycoses Study Group (EORTC/MSG). Among the patient serum samples, the first positive GM or BDG testsample and the median sample of GM or BDG test for negative patients were used to detect DNA levels by RT-PCR method (LightCycler 480, Roche Molecular Biochemicals, Meylan, France) using a commercial kit (Way2Gene Fungi; Genmar, İzmir, Turkey).Results: When the proven and probable IA group were considered as real patients, sensitivity of Aspergillus-specific DNA test was 90%,specificity was 73.3%, positive predictive value was 81.8%, and negative predictive value was 84.6%.Conclusion: This study found that searching for specific DNA by RT-PCR method has a sensitivity as high as the GM test. Althoughspecificity was rather low, it was concluded that it can be used jointly with GM and BDG tests after decreasing contamination by severelaboratory applications.

Makale detayları

Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis

Yazarlar: Gülçin BÖLÜK | Esra KAZAK | Fahir ÖZKALEMKAŞ | Beyza ENER | Halis AKALIN | Harun AĞCA | Yildiz OKUTURLAR | Kürşad KESKİN | Başak BURGAZLIOĞLU | Ridvan ALİ

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2016 Vol.46 Issue 2, pp.335-342

Belge Tipi: Makale

Yayın Tarihi: 3/2016

Belge Dili: İngilizce

Document ID (Org No.): 2000752181

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Gülçin BÖLÜK | Esra KAZAK | Fahir ÖZKALEMKAŞ | Beyza ENER | Halis AKALIN | Harun AĞCA | Yildiz OKUTURLAR | Kürşad KESKİN | Başak BURGAZLIOĞLU | Ridvan ALİ

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2016 Vol.46 Issue 2, pp.335-342

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000752181

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144