The Metabolic Effects of High-fructose Corn Syrup in Dental Tissues and Parotid Glands of Rats and the Therapeutic Effect of Alpha Lipoic Acid

Özge Kam Hepdeniz; Uğur Burak Temel; Fatma Nihan Cankara; Özlem Özmen

Makale detayları

The Metabolic Effects of High-fructose Corn Syrup in Dental Tissues and Parotid Glands of Rats and the Therapeutic Effect of Alpha Lipoic Acid

Yazarlar:

Özge Kam Hepdeniz | Uğur Burak Temel | Fatma Nihan Cankara | Özlem Özmen

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348374

pISSN:

2149-9063

Anahtar kelimeler:

Özet:

 Objective: This study aimed to examine the metabolic effects of high fructose corn syrup HFCS in dental tissues and parotid glands of rats and antioxidant defense of alpha-lipoic acid ALA in HFCS-induced effects. Materials and Methods: Female Wistar rats n24 divided into three groups group 1: negative control, group 2: positive control, and group 3: ALA with each group consisting of 8 animals. Rats in groups 2 and 3 were subjected to HFCS for 10 weeks. ALA was given to the animals in group 3 for the last 6 weeks of the experiment. The rats were euthanized by cervical dislocation. The tissues were prepared for histopathological, and immunohistochemical caspase-3, caspase-8 and bone moprhogenetic protein-2 BMP-2 evaluations. Data were analyzed by Kruskal-Wallis non-parametric data and one-way ANOVA tests parametric data p0.05. Results: Hyperemia and neutrophil leukocyte infiltrations in dental tissues and sialadenitis characterized by severe inflammatory reactions in the parotid gland were observed in group 2. After the ALA treatment, degenerative changes were decreased in both tissues, and the gland recovered an essentially normal appearance. Group 3 exhibited significantly lower inflammatory scores than the group 2 in terms of dental pulp p0.044. Both BMP-2 values and caspase-3 cell counts of the gingival tissue were significantly lower in group 3 compared to group 2 p0.01. Conclusion: The vascular and pulpal tissue damage caused by corn syrup was determined in this study. It was concluded that ALA could be a potential antioxidant against the harmful consequences of corn syrup consumption.

 Objective: This study aimed to examine the metabolic effects of high fructose corn syrup HFCS in dental tissues and parotid glands of rats and antioxidant defense of alpha-lipoic acid ALA in HFCS-induced effects. Materials and Methods: Female Wistar rats n24 divided into three groups group 1: negative control, group 2: positive control, and group 3: ALA with each group consisting of 8 animals. Rats in groups 2 and 3 were subjected to HFCS for 10 weeks. ALA was given to the animals in group 3 for the last 6 weeks of the experiment. The rats were euthanized by cervical dislocation. The tissues were prepared for histopathological, and immunohistochemical caspase-3, caspase-8 and bone moprhogenetic protein-2 BMP-2 evaluations. Data were analyzed by Kruskal-Wallis non-parametric data and one-way ANOVA tests parametric data p0.05. Results: Hyperemia and neutrophil leukocyte infiltrations in dental tissues and sialadenitis characterized by severe inflammatory reactions in the parotid gland were observed in group 2. After the ALA treatment, degenerative changes were decreased in both tissues, and the gland recovered an essentially normal appearance. Group 3 exhibited significantly lower inflammatory scores than the group 2 in terms of dental pulp p0.044. Both BMP-2 values and caspase-3 cell counts of the gingival tissue were significantly lower in group 3 compared to group 2 p0.01. Conclusion: The vascular and pulpal tissue damage caused by corn syrup was determined in this study. It was concluded that ALA could be a potential antioxidant against the harmful consequences of corn syrup consumption.

 Objective: This study aimed to examine the metabolic effects of high fructose corn syrup HFCS in dental tissues and parotid glands of rats and antioxidant defense of alpha-lipoic acid ALA in HFCS-induced effects. Materials and Methods: Female Wistar rats n24 divided into three groups group 1: negative control, group 2: positive control, and group 3: ALA with each group consisting of 8 animals. Rats in groups 2 and 3 were subjected to HFCS for 10 weeks. ALA was given to the animals in group 3 for the last 6 weeks of the experiment. The rats were euthanized by cervical dislocation. The tissues were prepared for histopathological, and immunohistochemical caspase-3, caspase-8 and bone moprhogenetic protein-2 BMP-2 evaluations. Data were analyzed by Kruskal-Wallis non-parametric data and one-way ANOVA tests parametric data p0.05. Results: Hyperemia and neutrophil leukocyte infiltrations in dental tissues and sialadenitis characterized by severe inflammatory reactions in the parotid gland were observed in group 2. After the ALA treatment, degenerative changes were decreased in both tissues, and the gland recovered an essentially normal appearance. Group 3 exhibited significantly lower inflammatory scores than the group 2 in terms of dental pulp p0.044. Both BMP-2 values and caspase-3 cell counts of the gingival tissue were significantly lower in group 3 compared to group 2 p0.01. Conclusion: The vascular and pulpal tissue damage caused by corn syrup was determined in this study. It was concluded that ALA could be a potential antioxidant against the harmful consequences of corn syrup consumption.

 Objective: This study aimed to examine the metabolic effects of high fructose corn syrup HFCS in dental tissues and parotid glands of rats and antioxidant defense of alpha-lipoic acid ALA in HFCS-induced effects. Materials and Methods: Female Wistar rats n24 divided into three groups group 1: negative control, group 2: positive control, and group 3: ALA with each group consisting of 8 animals. Rats in groups 2 and 3 were subjected to HFCS for 10 weeks. ALA was given to the animals in group 3 for the last 6 weeks of the experiment. The rats were euthanized by cervical dislocation. The tissues were prepared for histopathological, and immunohistochemical caspase-3, caspase-8 and bone moprhogenetic protein-2 BMP-2 evaluations. Data were analyzed by Kruskal-Wallis non-parametric data and one-way ANOVA tests parametric data p0.05. Results: Hyperemia and neutrophil leukocyte infiltrations in dental tissues and sialadenitis characterized by severe inflammatory reactions in the parotid gland were observed in group 2. After the ALA treatment, degenerative changes were decreased in both tissues, and the gland recovered an essentially normal appearance. Group 3 exhibited significantly lower inflammatory scores than the group 2 in terms of dental pulp p0.044. Both BMP-2 values and caspase-3 cell counts of the gingival tissue were significantly lower in group 3 compared to group 2 p0.01. Conclusion: The vascular and pulpal tissue damage caused by corn syrup was determined in this study. It was concluded that ALA could be a potential antioxidant against the harmful consequences of corn syrup consumption.

Makale detayları

The Metabolic Effects of High-fructose Corn Syrup in Dental Tissues and Parotid Glands of Rats and the Therapeutic Effect of Alpha Lipoic Acid

Yazarlar:

Özge Kam Hepdeniz | Uğur Burak Temel | Fatma Nihan Cankara | Özlem Özmen

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348374

pISSN:

2149-9063

Alpha-lipoic acid

dental pulp

high-fructose corn syrup

parotid gland

rats

Sayı

Makale detayları

The Metabolic Effects of High-fructose Corn Syrup in Dental Tissues and Parotid Glands of Rats and the Therapeutic Effect of Alpha Lipoic Acid

Yazarlar: Özge Kam Hepdeniz | Uğur Burak Temel | Fatma Nihan Cankara | Özlem Özmen

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Dentistry (Other)

Dergi: Meandros Medical And Dental Journal Vol.24 Issue 1, pp.39-45

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001348374

pISSN: 2149-9063

Alpha-lipoic acid

dental pulp

high-fructose corn syrup

parotid gland

rats

Sayı

Yazarlar:

Özge Kam Hepdeniz | Uğur Burak Temel | Fatma Nihan Cankara | Özlem Özmen

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001348374

pISSN:

2149-9063