Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Embryos

Hakan SAĞIRKAYA

Makale detayları

Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Embryos

Yazarlar:

Hakan SAĞIRKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 3, pp.623-631

Belge Tipi:

Makale

Yayın Tarihi:

2/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769556

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The aim of this study was to investigate the possibility of producing chimeric bovine embryos by reaggregating blastomeres of disaggregated day 4 bovine embryos. Oocytes were obtained from a slaughterhouse. Selected oocytes were matured, fertilized and cultured in vitro. Fertilization day was taken as day 0. Blastomeres of day 4 embryos were disaggregated and then pooled together. Chimeric embryos were randomly constructed by reaggregating blastomeres in empty zonae pellucidae. Non-chimeric handled control embryos were produced by transferring blastomeres from each embryo into their own empty zonae pellucidae. A total of 79 day 4 cattle embryos were used for disaggregation of blastomeres, and 27 chimeric and 12 non-chimeric handled control embryos were obtained from them. The rates of blastocyst formation of chimeric and non-chimeric handled control embryos were 40.7% (11/27) and 50.0% (6/12), respectively, and the difference between them was not statistically important (P > 0.05). These results showed the possibility of producing chimeric cattle embryos by reaggregating blastomeres obtained from day 4 embryos.

 

The aim of this study was to investigate the possibility of producing chimeric bovine embryos by reaggregating blastomeres of disaggregated day 4 bovine embryos. Oocytes were obtained from a slaughterhouse. Selected oocytes were matured, fertilized and cultured in vitro. Fertilization day was taken as day 0. Blastomeres of day 4 embryos were disaggregated and then pooled together. Chimeric embryos were randomly constructed by reaggregating blastomeres in empty zonae pellucidae. Non-chimeric handled control embryos were produced by transferring blastomeres from each embryo into their own empty zonae pellucidae. A total of 79 day 4 cattle embryos were used for disaggregation of blastomeres, and 27 chimeric and 12 non-chimeric handled control embryos were obtained from them. The rates of blastocyst formation of chimeric and non-chimeric handled control embryos were 40.7% (11/27) and 50.0% (6/12), respectively, and the difference between them was not statistically important (P > 0.05). These results showed the possibility of producing chimeric cattle embryos by reaggregating blastomeres obtained from day 4 embryos.

Makale detayları

Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Embryos

Yazarlar:

Hakan SAĞIRKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 3, pp.623-631

Belge Tipi:

Makale

Yayın Tarihi:

2/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769556

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Embryo

in vitro fertilization

chimera

cattle

Sayı

Makale detayları

Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Embryos

Yazarlar: Hakan SAĞIRKAYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 3, pp.623-631

Belge Tipi: Makale

Yayın Tarihi: 2/2004

Belge Dili: Türkçe

Document ID (Org No.): 2000769556

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Embryo

in vitro fertilization

chimera

cattle

Sayı

Yazarlar:

Hakan SAĞIRKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2004 Vol.28 Issue 3, pp.623-631

Belge Tipi:

Makale

Yayın Tarihi:

2/2004

Belge Dili:

Türkçe

Document ID (Org No.):

2000769556

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128