Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration

Murat KASAP

Makale detayları

Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration

Yazarlar:

Murat KASAP

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2006 Vol.36 Issue 6, pp.383-386

Belge Tipi:

Makale

Yayın Tarihi:

5/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000756123

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

A commonly used method to measure the amount of DNA in nucleic acid preparations is to use UV irradiation. However, if the DNA sample contains impurities, measuring UV irradiation is misleading. A quick way to estimate the amount of DNA in such samples is to analyze spot densities created by UV-induced fluorescence emitted by ethidium bromide. The amount of DNA is proportional to the amount of fluorescence. In many references, the spot density analysis is recommended for rough estimation of DNA concentration and is done by visual examination and comparison of the densities of each spot with the densities of DNA standards. Herein, we used a computer-integrated software to test the reliability of such a method, and showed that the analysis is highly reliable and simple.

 

A commonly used method to measure the amount of DNA in nucleic acid preparations is to use UV irradiation. However, if the DNA sample contains impurities, measuring UV irradiation is misleading. A quick way to estimate the amount of DNA in such samples is to analyze spot densities created by UV-induced fluorescence emitted by ethidium bromide. The amount of DNA is proportional to the amount of fluorescence. In many references, the spot density analysis is recommended for rough estimation of DNA concentration and is done by visual examination and comparison of the densities of each spot with the densities of DNA standards. Herein, we used a computer-integrated software to test the reliability of such a method, and showed that the analysis is highly reliable and simple.

Makale detayları

Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration

Yazarlar: Murat KASAP

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2006 Vol.36 Issue 6, pp.383-386

Belge Tipi: Makale

Yayın Tarihi: 5/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000756123

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

DNA concentration

spot density analysis

ethidium bromide

Sayı

Yazarlar:

Murat KASAP

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2006 Vol.36 Issue 6, pp.383-386

Belge Tipi:

Makale

Yayın Tarihi:

5/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000756123

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144