Can plasma-free DNA concentration be a diagnostic tool in critically ill septic patients?

Başak Ceyda MEÇO*; Fatma Handan CUHRUK; Melek TULUNAY; Mehmet ORAL; Mustafa Necmettin ÜNAL

Makale detayları

Can plasma-free DNA concentration be a diagnostic tool in critically ill septic patients?

Yazarlar:

Başak Ceyda MEÇO* | Fatma Handan CUHRUK | Melek TULUNAY | Mehmet ORAL | Mustafa Necmettin ÜNAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 1, pp.150-155

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000753905

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To investigate the diagnostic value of plasma-free DNA concentration (PF-DNA) in septic patients compared to nonseptic patients and to correlate it with clinical outcome.   Materials and methods: Forty-two mechanically ventilated consecutive patients (11 septic, 31 nonseptic) admitted to the intensive care unit (ICU) were recruited. On admission, besides PF-DNA concentration, APACHE II and SOFA scores and serum C-reactive protein (CRP), procalcitonin (PCT), and serum lipid concentrations, together with clinical outcome, were assessed. Plasma samples from 11 volunteers were also collected. Appropriate statistics were used for analysis.   Results: Median PF-DNA concentrations on admission were significantly higher in septic patients compared to nonseptic patients [14,285 (48-180,311) GE/mL versus 546.5 (0-7674) GE/mL, P < 0.0001]. For distinguishing septic and nonseptic patients on admission, the area under the curve obtained for PF-DNA concentration was 0.9 (sensitivity 84%, specificity 95%; cutoff 4083 GE/mL). There were no significant differences between PF-DNA concentrations of nonsurvivors and survivors. Additionally, DNA concentration demonstrated a significant correlation with CRP, PCT, and high-density lipoprotein concentrations.   Conclusion: Plasma DNA seems to be a potentially valuable tool to confirm sepsis diagnosis upon ICU admission.

 

To investigate the diagnostic value of plasma-free DNA concentration (PF-DNA) in septic patients compared to nonseptic patients and to correlate it with clinical outcome.   Materials and methods: Forty-two mechanically ventilated consecutive patients (11 septic, 31 nonseptic) admitted to the intensive care unit (ICU) were recruited. On admission, besides PF-DNA concentration, APACHE II and SOFA scores and serum C-reactive protein (CRP), procalcitonin (PCT), and serum lipid concentrations, together with clinical outcome, were assessed. Plasma samples from 11 volunteers were also collected. Appropriate statistics were used for analysis.   Results: Median PF-DNA concentrations on admission were significantly higher in septic patients compared to nonseptic patients [14,285 (48-180,311) GE/mL versus 546.5 (0-7674) GE/mL, P < 0.0001]. For distinguishing septic and nonseptic patients on admission, the area under the curve obtained for PF-DNA concentration was 0.9 (sensitivity 84%, specificity 95%; cutoff 4083 GE/mL). There were no significant differences between PF-DNA concentrations of nonsurvivors and survivors. Additionally, DNA concentration demonstrated a significant correlation with CRP, PCT, and high-density lipoprotein concentrations.   Conclusion: Plasma DNA seems to be a potentially valuable tool to confirm sepsis diagnosis upon ICU admission.

Makale detayları

Can plasma-free DNA concentration be a diagnostic tool in critically ill septic patients?

Yazarlar:

Başak Ceyda MEÇO* | Fatma Handan CUHRUK | Melek TULUNAY | Mehmet ORAL | Mustafa Necmettin ÜNAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 1, pp.150-155

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000753905

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Plasma-free DNA

critical illness

sepsis

diagnosis

Sayı

Makale detayları

Can plasma-free DNA concentration be a diagnostic tool in critically ill septic patients?

Yazarlar: Başak Ceyda MEÇO* | Fatma Handan CUHRUK | Melek TULUNAY | Mehmet ORAL | Mustafa Necmettin ÜNAL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 1, pp.150-155

Belge Tipi: Makale

Yayın Tarihi: 12/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000753905

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Plasma-free DNA

critical illness

sepsis

diagnosis

Sayı

Yazarlar:

Başak Ceyda MEÇO* | Fatma Handan CUHRUK | Melek TULUNAY | Mehmet ORAL | Mustafa Necmettin ÜNAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 1, pp.150-155

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000753905

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144