Application of Western Blotting and Enzyme Linked Immunosorbent Assay (ELISA) for the Diagnosis of Dicrocoelium dendriticum in Sheep Using Excretory Secretory (E/S) Antigens

Sami ŞİMŞEK; Ergün KÖROĞLU; Armağan Erdem ÜTÜK

Makale detayları

Application of Western Blotting and Enzyme Linked Immunosorbent Assay (ELISA) for the Diagnosis of Dicrocoelium dendriticum in Sheep Using Excretory Secretory (E/S) Antigens

Yazarlar:

Sami ŞİMŞEK | Ergün KÖROĞLU | Armağan Erdem ÜTÜK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.113-119

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000768569

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into 2 groups as positive (n = 23) and negative (n = 17) for D. dendriticum and the sera were tested using Western blotting. The bands obtained from the sera of positive and negative groups were compared and the molecular weight of specific protein band for D. dendriticum infection was determined as 205 kDa. On the other hand, the ELISA technique was used to study the response of IgG antibodies against E/S antigens of D. dendriticum in the sera of 40 sheep. The sensitivity and specificity of the test were 82.6% and 76.4%, respectively.

 

Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into 2 groups as positive (n = 23) and negative (n = 17) for D. dendriticum and the sera were tested using Western blotting. The bands obtained from the sera of positive and negative groups were compared and the molecular weight of specific protein band for D. dendriticum infection was determined as 205 kDa. On the other hand, the ELISA technique was used to study the response of IgG antibodies against E/S antigens of D. dendriticum in the sera of 40 sheep. The sensitivity and specificity of the test were 82.6% and 76.4%, respectively.

Makale detayları

Application of Western Blotting and Enzyme Linked Immunosorbent Assay (ELISA) for the Diagnosis of Dicrocoelium dendriticum in Sheep Using Excretory Secretory (E/S) Antigens

Yazarlar: Sami ŞİMŞEK | Ergün KÖROĞLU | Armağan Erdem ÜTÜK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.113-119

Belge Tipi: Makale

Yayın Tarihi: 12/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000768569

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Dicrocoelium dendriticum

E/S antigen

sheep

Western blotting

ELISA

Sayı

Yazarlar:

Sami ŞİMŞEK | Ergün KÖROĞLU | Armağan Erdem ÜTÜK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.113-119

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000768569

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128