Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration

Tolga Göksan; İlknur Ak; Cenker Kılıç

Makale detayları

Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration

Yazarlar:

Tolga Göksan | İlknur Ak | Cenker Kılıç

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838364

Sıklık:

Üç Aylık

pISSN:

1303-2712

Anahtar kelimeler:

Özet:

 

In the present study, the effects of various inorganic nitrogen sources, light intensities, aeration rates and especially vitamins were investigated in vegetative Haematococcus pluvialis Flotow cultures. The best growth among the nitrogen sources was achieved in NaNO3 (1.0 g/L) and KNO3 (0.5 g/L) with cell densities of 25.3 and 26.3x104 cells/ml respectively. In vitamin trials, the highest cell numbers were found in 0.1 (0.3 µM), 1.0 (4 µM) and 0.1 mg/L (0.75 µM) concentrations for thiamin, biotin and B12 respectively. It was also seen that the use of thiamin alone was sufficient instead of using the vitamin mix. Cell growth was significantly higher in 75 and 150 µmol photon m-2 s-1 illuminations compared to 20 and 40 µmol photon m-2 s-1. As for aeration, optimum airflow rate was 1.0 lpm, while supra-optimum levels ceased the growth, cell diameter and pigment content increased. So, it may play a role as a secondary stress factor on H. pluvialis cells in reddening stage. 	 	 		 .

 

In the present study, the effects of various inorganic nitrogen sources, light intensities, aeration rates and especially vitamins were investigated in vegetative Haematococcus pluvialis Flotow cultures. The best growth among the nitrogen sources was achieved in NaNO3 (1.0 g/L) and KNO3 (0.5 g/L) with cell densities of 25.3 and 26.3x104 cells/ml respectively. In vitamin trials, the highest cell numbers were found in 0.1 (0.3 µM), 1.0 (4 µM) and 0.1 mg/L (0.75 µM) concentrations for thiamin, biotin and B12 respectively. It was also seen that the use of thiamin alone was sufficient instead of using the vitamin mix. Cell growth was significantly higher in 75 and 150 µmol photon m-2 s-1 illuminations compared to 20 and 40 µmol photon m-2 s-1. As for aeration, optimum airflow rate was 1.0 lpm, while supra-optimum levels ceased the growth, cell diameter and pigment content increased. So, it may play a role as a secondary stress factor on H. pluvialis cells in reddening stage. 	 	 		 .

Makale detayları

Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration

Yazarlar:

Tolga Göksan | İlknur Ak | Cenker Kılıç

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838364

Sıklık:

Üç Aylık

pISSN:

1303-2712

Aeration

Culture medium

Haematococcus pluvialis

nitrogen

vitamins

Sayı

Makale detayları

Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration

Yazarlar: Tolga Göksan | İlknur Ak | Cenker Kılıç

Yayıncı: Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi: Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 3

Belge Tipi: Makale

Yayın Tarihi: 5/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000838364

Sıklık: Üç Aylık

pISSN: 1303-2712

Aeration

Culture medium

Haematococcus pluvialis

nitrogen

vitamins

Sayı

Yazarlar:

Tolga Göksan | İlknur Ak | Cenker Kılıç

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

5/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838364

Sıklık:

Üç Aylık

pISSN:

1303-2712