Inhibition effect of Gly-Arg-Gly-Asp-Ser (GRGDS) and Arg-Gly-Asp (RGD) Bioactive Peptides on Angiotensin-Converting Enzyme Activity Purified from Human Serum

Resul Adanaş; Vedat Türkoğlu; Zehra Baş

Makale detayları

Inhibition effect of Gly-Arg-Gly-Asp-Ser (GRGDS) and Arg-Gly-Asp (RGD) Bioactive Peptides on Angiotensin-Converting Enzyme Activity Purified from Human Serum

Yazarlar:

Resul Adanaş | Vedat Türkoğlu | Zehra Baş

Yayıncı:

Igdir University

Ana Başlıklar:

Organik Kimya (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2847-2860

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302956

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Angiotensin-converting enzyme ACE, EC 3.4.15.1 is a physiological target for researching new antihypertensive drugs, as it is a substantial enzyme in the regulation of blood pressure. Herein, ACE was purified from human serum with affinity chromatography. Vmax and KM values were found as 60.98 µmolminmL and 0.34 mM, respectively. The effects of Gly-Arg-Gly-Asp-Ser GRGDS and Arg-Gly-Asp RGD bioactive peptides on purified ACE were researched. Also, captopril, a specific ACE inhibitory, was used as a reference inhibitor. Bioactive peptides, GRGDS and RGD, demonstrated the inhibitory effect on purified ACE with IC50 values of 46.39 µM and 456.46 µM, respectively. Ki values and kind of inhibition for GRGDS and RGD by the Lineweaver-Burk chart were found. The kind of inhibitory for these bioactive peptides was calculated as reversible-competitive inhibitory. Ki values for GRGDS and RGD were obtained as 93.28 µM and 435.67 µM, respectively. The IC50 value of captopril was designated as 1.57 nM. The inhibitory kind of captopril was designated as reversible non-competitive inhibitory and the Ki value was 0.99 nM. In this study, it was concluded that RGD and GRGDS bioactive peptides have the potential to be utilized as ACE inhibitors.

 Angiotensin-converting enzyme ACE, EC 3.4.15.1 is a physiological target for researching new antihypertensive drugs, as it is a substantial enzyme in the regulation of blood pressure. Herein, ACE was purified from human serum with affinity chromatography. Vmax and KM values were found as 60.98 µmolminmL and 0.34 mM, respectively. The effects of Gly-Arg-Gly-Asp-Ser GRGDS and Arg-Gly-Asp RGD bioactive peptides on purified ACE were researched. Also, captopril, a specific ACE inhibitory, was used as a reference inhibitor. Bioactive peptides, GRGDS and RGD, demonstrated the inhibitory effect on purified ACE with IC50 values of 46.39 µM and 456.46 µM, respectively. Ki values and kind of inhibition for GRGDS and RGD by the Lineweaver-Burk chart were found. The kind of inhibitory for these bioactive peptides was calculated as reversible-competitive inhibitory. Ki values for GRGDS and RGD were obtained as 93.28 µM and 435.67 µM, respectively. The IC50 value of captopril was designated as 1.57 nM. The inhibitory kind of captopril was designated as reversible non-competitive inhibitory and the Ki value was 0.99 nM. In this study, it was concluded that RGD and GRGDS bioactive peptides have the potential to be utilized as ACE inhibitors.

 Angiotensin-converting enzyme ACE, EC 3.4.15.1 is a physiological target for researching new antihypertensive drugs, as it is a substantial enzyme in the regulation of blood pressure. Herein, ACE was purified from human serum with affinity chromatography. Vmax and KM values were found as 60.98 µmolminmL and 0.34 mM, respectively. The effects of Gly-Arg-Gly-Asp-Ser GRGDS and Arg-Gly-Asp RGD bioactive peptides on purified ACE were researched. Also, captopril, a specific ACE inhibitory, was used as a reference inhibitor. Bioactive peptides, GRGDS and RGD, demonstrated the inhibitory effect on purified ACE with IC50 values of 46.39 µM and 456.46 µM, respectively. Ki values and kind of inhibition for GRGDS and RGD by the Lineweaver-Burk chart were found. The kind of inhibitory for these bioactive peptides was calculated as reversible-competitive inhibitory. Ki values for GRGDS and RGD were obtained as 93.28 µM and 435.67 µM, respectively. The IC50 value of captopril was designated as 1.57 nM. The inhibitory kind of captopril was designated as reversible non-competitive inhibitory and the Ki value was 0.99 nM. In this study, it was concluded that RGD and GRGDS bioactive peptides have the potential to be utilized as ACE inhibitors.

 Angiotensin-converting enzyme ACE, EC 3.4.15.1 is a physiological target for researching new antihypertensive drugs, as it is a substantial enzyme in the regulation of blood pressure. Herein, ACE was purified from human serum with affinity chromatography. Vmax and KM values were found as 60.98 µmolminmL and 0.34 mM, respectively. The effects of Gly-Arg-Gly-Asp-Ser GRGDS and Arg-Gly-Asp RGD bioactive peptides on purified ACE were researched. Also, captopril, a specific ACE inhibitory, was used as a reference inhibitor. Bioactive peptides, GRGDS and RGD, demonstrated the inhibitory effect on purified ACE with IC50 values of 46.39 µM and 456.46 µM, respectively. Ki values and kind of inhibition for GRGDS and RGD by the Lineweaver-Burk chart were found. The kind of inhibitory for these bioactive peptides was calculated as reversible-competitive inhibitory. Ki values for GRGDS and RGD were obtained as 93.28 µM and 435.67 µM, respectively. The IC50 value of captopril was designated as 1.57 nM. The inhibitory kind of captopril was designated as reversible non-competitive inhibitory and the Ki value was 0.99 nM. In this study, it was concluded that RGD and GRGDS bioactive peptides have the potential to be utilized as ACE inhibitors.

Makale detayları

Inhibition effect of Gly-Arg-Gly-Asp-Ser (GRGDS) and Arg-Gly-Asp (RGD) Bioactive Peptides on Angiotensin-Converting Enzyme Activity Purified from Human Serum

Yazarlar:

Resul Adanaş | Vedat Türkoğlu | Zehra Baş

Yayıncı:

Igdir University

Ana Başlıklar:

Organik Kimya (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2847-2860

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302956

pISSN:

2536-4618

Angiotensin-converting enzyme (ACE)

bioactive peptides

Gly-Arg-Gly-Asp-Ser

Arg-Gly-Asp

Inhibition Purification

Sayı

Makale detayları

Inhibition effect of Gly-Arg-Gly-Asp-Ser (GRGDS) and Arg-Gly-Asp (RGD) Bioactive Peptides on Angiotensin-Converting Enzyme Activity Purified from Human Serum

Yazarlar: Resul Adanaş | Vedat Türkoğlu | Zehra Baş

Yayıncı: Igdir University

Ana Başlıklar: Organik Kimya (Diğer)

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2847-2860

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302956

pISSN: 2536-4618

Angiotensin-converting enzyme (ACE)

bioactive peptides

Gly-Arg-Gly-Asp-Ser

Arg-Gly-Asp

Inhibition Purification

Sayı

Yazarlar:

Resul Adanaş | Vedat Türkoğlu | Zehra Baş

Yayıncı:

Igdir University

Ana Başlıklar:

Organik Kimya (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2847-2860

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302956

pISSN:

2536-4618