Investigation of the putative functional relevance of the IL-6 3'UTR genetic variants with athletic phenotype in Turkish triathletes

Dilek Pirim; Hasan Faruk Niş; Fatih Atilla Bağcı

Makale detayları

Investigation of the putative functional relevance of the IL-6 3'UTR genetic variants with athletic phenotype in Turkish triathletes

Yazarlar:

Dilek Pirim | Hasan Faruk Niş | Fatih Atilla Bağcı

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.151-160

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324504

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 Previous research suggests that genetic variants in the interleukin-6 IL-6 gene contribute to sport-related traits and athletic performance. We aimed to identify sequence variants in the IL-6 gene region comprising the 3 untranslated region UTR in the Turkish triathletes and sedentary individuals and assessed their putative roles in tendency to athletic phenotype. Sequence variants were identified in the Turkish triathletes n  47 and sedentary individu-als n  46 by Sanger sequencing. Allelegenotype frequencies and linkage disequilibrium LD patterns were calculated by the Haploview program. The functional significance of the detected variants was analyzed using in silico prediction tools. Four single nucleotide vari-ants rs13306435, rs747302620, rs2069849, rs13306436 were detected in saliva samples of the participants by sequencing the target region. Notably, rs13306436-3UTRIL-6 was only seen in the triathletes, while the exonic rs747302620 was observed in only sedentary group. Also, rs13306436GA causes lossgain sites for binding multiple miRNAs that may be associated with athletic performance. Our findings indicate that the 3UTRIL-6 may have functional relevance in determining sports talent. Future comprehensive studies focusing on the IL-6 gene in athletes may pave the way for not only determining the athletic status of the individuals but also have implications for translational medicine.

 Previous research suggests that genetic variants in the interleukin-6 IL-6 gene contribute to sport-related traits and athletic performance. We aimed to identify sequence variants in the IL-6 gene region comprising the 3 untranslated region UTR in the Turkish triathletes and sedentary individuals and assessed their putative roles in tendency to athletic phenotype. Sequence variants were identified in the Turkish triathletes n  47 and sedentary individu-als n  46 by Sanger sequencing. Allelegenotype frequencies and linkage disequilibrium LD patterns were calculated by the Haploview program. The functional significance of the detected variants was analyzed using in silico prediction tools. Four single nucleotide vari-ants rs13306435, rs747302620, rs2069849, rs13306436 were detected in saliva samples of the participants by sequencing the target region. Notably, rs13306436-3UTRIL-6 was only seen in the triathletes, while the exonic rs747302620 was observed in only sedentary group. Also, rs13306436GA causes lossgain sites for binding multiple miRNAs that may be associated with athletic performance. Our findings indicate that the 3UTRIL-6 may have functional relevance in determining sports talent. Future comprehensive studies focusing on the IL-6 gene in athletes may pave the way for not only determining the athletic status of the individuals but also have implications for translational medicine.

 Previous research suggests that genetic variants in the interleukin-6 IL-6 gene contribute to sport-related traits and athletic performance. We aimed to identify sequence variants in the IL-6 gene region comprising the 3 untranslated region UTR in the Turkish triathletes and sedentary individuals and assessed their putative roles in tendency to athletic phenotype. Sequence variants were identified in the Turkish triathletes n  47 and sedentary individu-als n  46 by Sanger sequencing. Allelegenotype frequencies and linkage disequilibrium LD patterns were calculated by the Haploview program. The functional significance of the detected variants was analyzed using in silico prediction tools. Four single nucleotide vari-ants rs13306435, rs747302620, rs2069849, rs13306436 were detected in saliva samples of the participants by sequencing the target region. Notably, rs13306436-3UTRIL-6 was only seen in the triathletes, while the exonic rs747302620 was observed in only sedentary group. Also, rs13306436GA causes lossgain sites for binding multiple miRNAs that may be associated with athletic performance. Our findings indicate that the 3UTRIL-6 may have functional relevance in determining sports talent. Future comprehensive studies focusing on the IL-6 gene in athletes may pave the way for not only determining the athletic status of the individuals but also have implications for translational medicine.

 Önceki araştırmalar, interlökin-6 IL-6 geni varyantlarının spor ile ilgili özelliklere ve atle-tik performansa katkı sağladığını ileri sürmektedir. Bu çalışmada, Türk triatletler ve sedanter bireylerde IL-6 geninin 3 translasyon olmayan bölgelerinde UTR dizi varyantlarını tanım-lamayı ve bunların atletik fenotipe yatkınlıktaki varsayılan rollerini değerlendirmeyi amaçla-dık. Türk triatletlerde n  47 ve sedanter bireylerde n  46 dizi varyantları Sanger dizi-leme ile tanımlanmıştır. Alelgenotip frekansları ve bağlantı dengesizliği LD örüntüleri Haploview programı ile hesaplanmıştır. Tespit edilen varyantların fonksiyonel önemleri in silico tahmin araçları kullanılarak analiz edilmiştir. Hedef gen bölgesinin dizilenmesi sonu-cunda, katılımcıların tükürük örneklerinde dört tek nükleotid varyantı rs13306435, rs747302620, rs2069849, rs13306436 tespit edilmiştir. rs13306436-3UTRIL-6 sadece triatletlerde görülürken, ekzonik rs747302620 sadece sedanter grupta gözlenmiştir. Ayrıca, rs13306436GA, miRNAların bağlanabileceği kayıpkazanç bölgeleri yaratarak atletil per-formans ile ilişkili olabilir. Bulgularımız, 3UTRIL-6nın sporcu yeteneğini belirlemede iş-levsel bir öneme sahip olabileceğini göstermektedir. Sporcularda IL-6 genine odaklı yapıla-cak gelecekteki kapsamlı çalışmalar, yalnızca bireylerin atletik durumlarının belirlenmesine değil, aynı zamanda translasyonel tıp için de çıkarımlara yol açabilir.

Makale detayları

Investigation of the putative functional relevance of the IL-6 3'UTR genetic variants with athletic phenotype in Turkish triathletes

Yazarlar:

Dilek Pirim | Hasan Faruk Niş | Fatih Atilla Bağcı

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.151-160

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324504

pISSN:

2528-9691

3'UTR

bioinformatics

SNV

sport genetics

Interleukin-6

Triathletes

Sayı

Makale detayları

Investigation of the putative functional relevance of the IL-6 3'UTR genetic variants with athletic phenotype in Turkish triathletes

Yazarlar: Dilek Pirim | Hasan Faruk Niş | Fatih Atilla Bağcı

Yayıncı: Trakya University

Ana Başlıklar: Genomics

Dergi: Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.151-160

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001324504

pISSN: 2528-9691

3'UTR

bioinformatics

SNV

sport genetics

Interleukin-6

Triathletes

Sayı

Yazarlar:

Dilek Pirim | Hasan Faruk Niş | Fatih Atilla Bağcı

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.151-160

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324504

pISSN:

2528-9691