Effect of Addition Saccharomyces cerevisiae Elicitor on Total Flavonoid Content and Antioxidant Activity of Gardenia jasminoides Cell Suspension Culture

Giovanny Okta Francisca; Exsyupransia Mursyanti

Makale detayları

Effect of Addition Saccharomyces cerevisiae Elicitor on Total Flavonoid Content and Antioxidant Activity of Gardenia jasminoides Cell Suspension Culture

Yazarlar:

Giovanny Okta Francisca | Exsyupransia Mursyanti

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 1, pp.23-35

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342530

pISSN:

1308-7576

Anahtar kelimeler:

Özet:

 G. jasminoides is a medicinal plant with diverse properties due to the various secondary metabolites including flavonoids. Flavonoid content in gardenia leaves can be increased through in vitro culture methods, such as CSC combined with elicitation. This process is carried out by adding an elicitor, which provides a stress condition in the culture to produce secondary metabolites. An example of an elicitor capable of increasing secondary metabolites is S. cerevisiae powder. Therefore, this study aimed to examine the morphology of gardenia leaves callus, measure the growth of CSC, determine the optimum elicitation time, and evaluate the most potent concentration of S. cerevisiae powder. The treatments carried out include variations in elicitation duration of 0, 2, 4, and 6 days as well as S. cerevisiae elicitor concentrations of 0, 2.5, 5, and 7.5. The results showed that the callus of gardenia leaves was friable and yellowish. Gardenia CSC showed two growth phases, namely exponential and stationary. The optimal elicitation period for maximizing total flavonoid content was six days, whereas the ideal period for achieving the highest antioxidant activity was two days. The highest levels of both flavonoids and antioxidant activity were observed with a 7.5 concentration of S. cerevisiae.

 G. jasminoides is a medicinal plant with diverse properties due to the various secondary metabolites including flavonoids. Flavonoid content in gardenia leaves can be increased through in vitro culture methods, such as CSC combined with elicitation. This process is carried out by adding an elicitor, which provides a stress condition in the culture to produce secondary metabolites. An example of an elicitor capable of increasing secondary metabolites is S. cerevisiae powder. Therefore, this study aimed to examine the morphology of gardenia leaves callus, measure the growth of CSC, determine the optimum elicitation time, and evaluate the most potent concentration of S. cerevisiae powder. The treatments carried out include variations in elicitation duration of 0, 2, 4, and 6 days as well as S. cerevisiae elicitor concentrations of 0, 2.5, 5, and 7.5. The results showed that the callus of gardenia leaves was friable and yellowish. Gardenia CSC showed two growth phases, namely exponential and stationary. The optimal elicitation period for maximizing total flavonoid content was six days, whereas the ideal period for achieving the highest antioxidant activity was two days. The highest levels of both flavonoids and antioxidant activity were observed with a 7.5 concentration of S. cerevisiae.

 G. jasminoides is a medicinal plant with diverse properties due to the various secondary metabolites including flavonoids. Flavonoid content in gardenia leaves can be increased through in vitro culture methods, such as CSC combined with elicitation. This process is carried out by adding an elicitor, which provides a stress condition in the culture to produce secondary metabolites. An example of an elicitor capable of increasing secondary metabolites is S. cerevisiae powder. Therefore, this study aimed to examine the morphology of gardenia leaves callus, measure the growth of CSC, determine the optimum elicitation time, and evaluate the most potent concentration of S. cerevisiae powder. The treatments carried out include variations in elicitation duration of 0, 2, 4, and 6 days as well as S. cerevisiae elicitor concentrations of 0, 2.5, 5, and 7.5. The results showed that the callus of gardenia leaves was friable and yellowish. Gardenia CSC showed two growth phases, namely exponential and stationary. The optimal elicitation period for maximizing total flavonoid content was six days, whereas the ideal period for achieving the highest antioxidant activity was two days. The highest levels of both flavonoids and antioxidant activity were observed with a 7.5 concentration of S. cerevisiae.

 G. jasminoides is a medicinal plant with diverse properties due to the various secondary metabolites including flavonoids. Flavonoid content in gardenia leaves can be increased through in vitro culture methods, such as CSC combined with elicitation. This process is carried out by adding an elicitor, which provides a stress condition in the culture to produce secondary metabolites. An example of an elicitor capable of increasing secondary metabolites is S. cerevisiae powder. Therefore, this study aimed to examine the morphology of gardenia leaves callus, measure the growth of CSC, determine the optimum elicitation time, and evaluate the most potent concentration of S. cerevisiae powder. The treatments carried out include variations in elicitation duration of 0, 2, 4, and 6 days as well as S. cerevisiae elicitor concentrations of 0, 2.5, 5, and 7.5. The results showed that the callus of gardenia leaves was friable and yellowish. Gardenia CSC showed two growth phases, namely exponential and stationary. The optimal elicitation period for maximizing total flavonoid content was six days, whereas the ideal period for achieving the highest antioxidant activity was two days. The highest levels of both flavonoids and antioxidant activity were observed with a 7.5 concentration of S. cerevisiae.

Makale detayları

Effect of Addition Saccharomyces cerevisiae Elicitor on Total Flavonoid Content and Antioxidant Activity of Gardenia jasminoides Cell Suspension Culture

Yazarlar:

Giovanny Okta Francisca | Exsyupransia Mursyanti

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 1, pp.23-35

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342530

pISSN:

1308-7576

Cell Suspension Culture (CSC)

DPPH Inhibition

Dry weight

Elicitation

Flavonoid

Sayı

Makale detayları

Effect of Addition Saccharomyces cerevisiae Elicitor on Total Flavonoid Content and Antioxidant Activity of Gardenia jasminoides Cell Suspension Culture

Yazarlar: Giovanny Okta Francisca | Exsyupransia Mursyanti

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Plant Biotechnology in Agriculture

Dergi: Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 1, pp.23-35

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001342530

pISSN: 1308-7576

Cell Suspension Culture (CSC)

DPPH Inhibition

Dry weight

Elicitation

Flavonoid

Sayı

Yazarlar:

Giovanny Okta Francisca | Exsyupransia Mursyanti

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Plant Biotechnology in Agriculture

Dergi:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 1, pp.23-35

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342530

pISSN:

1308-7576