Comparison of Biofilm Forming Properties of Staphylococcus aureus Isolates

Demet Gür Vural; İlknur Bıyık; Elif Gülsüm Torun; Yeliz Tanrıverdi Çaycı; Kemal Bilgin; Asuman Bırıncı

Makale detayları

Comparison of Biofilm Forming Properties of Staphylococcus aureus Isolates

Yazarlar:

Demet Gür Vural | İlknur Bıyık | Elif Gülsüm Torun | Yeliz Tanrıverdi Çaycı | Kemal Bilgin | Asuman Bırıncı

Yayıncı:

Duzce University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Sağlık Bilimlerinde Değer Vol.13 Issue 2, pp.245-249

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001289519

pISSN:

2792-0542

Anahtar kelimeler:

Özet:

 Aim: Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The aim of our study was to determine the biofilm forming potential of methicillin-susceptible S. aureus MSSA and methicillin-resistant S. aureus MRSA isolates and to observe the difference between them. Material and Methods: A total of 200 S. aureus strains 100 MRSA, 100 MSSA obtained from various clinical samples sent to the Microbiology laboratory were included in our study. Identification of isolates was determined by Vitek MS BioMerieux, France device and antibiotic susceptibility was determined by Vitek2 Compact BioMerieux, France automated system.. Biofilm production was studied using the Microtitration plate method.The biofilm forming ability of MSSA and MRSA strains was statistically examined. Statistical significance level was taken as p0.05. Results: We found 51 biofilm formation among all S.aureus strains included in the study. Biofilm formation was observed in 34 of MSSA strains and 68 of MRSA strains. There was a statistically significant difference in biofilm formation between MRSA and MSSA groups p0.05. Conclusion: We determined that the biofilm forming ability of MRSA strains was significantly higher than MSSA, in our study. MRSA and MSSA strains indicates that these strains have a high ability to persist in the hospital setting and increase the risk of disease development in hospitalized patients.

 Aim: Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The aim of our study was to determine the biofilm forming potential of methicillin-susceptible S. aureus MSSA and methicillin-resistant S. aureus MRSA isolates and to observe the difference between them. Material and Methods: A total of 200 S. aureus strains 100 MRSA, 100 MSSA obtained from various clinical samples sent to the Microbiology laboratory were included in our study. Identification of isolates was determined by Vitek MS BioMerieux, France device and antibiotic susceptibility was determined by Vitek2 Compact BioMerieux, France automated system.. Biofilm production was studied using the Microtitration plate method.The biofilm forming ability of MSSA and MRSA strains was statistically examined. Statistical significance level was taken as p0.05. Results: We found 51 biofilm formation among all S.aureus strains included in the study. Biofilm formation was observed in 34 of MSSA strains and 68 of MRSA strains. There was a statistically significant difference in biofilm formation between MRSA and MSSA groups p0.05. Conclusion: We determined that the biofilm forming ability of MRSA strains was significantly higher than MSSA, in our study. MRSA and MSSA strains indicates that these strains have a high ability to persist in the hospital setting and increase the risk of disease development in hospitalized patients.

 Aim: Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The aim of our study was to determine the biofilm forming potential of methicillin-susceptible S. aureus MSSA and methicillin-resistant S. aureus MRSA isolates and to observe the difference between them. Material and Methods: A total of 200 S. aureus strains 100 MRSA, 100 MSSA obtained from various clinical samples sent to the Microbiology laboratory were included in our study. Identification of isolates was determined by Vitek MS BioMerieux, France device and antibiotic susceptibility was determined by Vitek2 Compact BioMerieux, France automated system.. Biofilm production was studied using the Microtitration plate method.The biofilm forming ability of MSSA and MRSA strains was statistically examined. Statistical significance level was taken as p0.05. Results: We found 51 biofilm formation among all S.aureus strains included in the study. Biofilm formation was observed in 34 of MSSA strains and 68 of MRSA strains. There was a statistically significant difference in biofilm formation between MRSA and MSSA groups p0.05. Conclusion: We determined that the biofilm forming ability of MRSA strains was significantly higher than MSSA, in our study. MRSA and MSSA strains indicates that these strains have a high ability to persist in the hospital setting and increase the risk of disease development in hospitalized patients.

 Amaç: Staphylococcus aureus, hastane kaynaklı ve toplum kökenli enfeksiyonların önemli bir nedenidir. S. aureusun tıbbi implantlara ve konak dokuya tutunması ve olgun bir biyofilmin oluşması, kronik enfeksiyonların kalıcılığında önemli bir rol oynamaktadır. Çalışmamızın amacı, metisiline duyarlı S. aureus MSSA ve metisiline dirençli S. aureus MRSA izolatlarının biyofilm oluşturma potansiyelini belirlemek ve aralarındaki farkı gözlemlemektir. Gereç ve Yöntemler: Çalışmamıza Mikrobiyoloji laboratuvarına gönderilen çeşitli klinik örneklerden elde edilen toplam 200 S. aureus suşu 100 MRSA ve 100 MSSA dahil edilmiştir. İzolatların tanımlanması Vitek MS BioMerieux, Fransa cihazı ile antibiyotik duyarlılıkları Vitek2 Compakt BioMerieux, Fransa otomatize sistemi ile belirlendi. Biyofilm üretimi, Mikrotitrasyon plak yöntemi kullanılarak çalışıldı. MSSA ve MRSA suşlarının biyofilm oluşturma yeteneği istatistiksel olarak incelendi. İstatistiksel anlamlılık düzeyi p0,05 olarak alındı. Bulgular: Çalışmaya dahil edilen tüm S.aureus suşları arasında 51 oranında biyofilm oluşumu saptadık. MSSA suşlarının 34 ünde, MRSA suşlarının 68 inde biyofilm oluşumu gözlenmiştir. MRSA ve MSSA grupları arasında biyofilm oluşturma açısından istatistiksel olarak anlamlı bir fark saptanmıştır p0,05. Sonuç: Çalışmamızda MRSA suşlarının biyofilm oluşturma yeteneğinin MSSAlara göre anlamlı derecede yüksek olduğu saptanmıştır. MRSA ve MSSA suşlarının oluşturduğu biyofilm, bu suşların hastane ortamında kalıcı olma kabiliyetinin yüksek olduğunu ve hastanede yatan hastalarda tedaviye başarısızlık olasılığını artırdığını gösterir.

Makale detayları

Comparison of Biofilm Forming Properties of Staphylococcus aureus Isolates

Yazarlar:

Demet Gür Vural | İlknur Bıyık | Elif Gülsüm Torun | Yeliz Tanrıverdi Çaycı | Kemal Bilgin | Asuman Bırıncı

Yayıncı:

Duzce University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Sağlık Bilimlerinde Değer Vol.13 Issue 2, pp.245-249

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001289519

pISSN:

2792-0542

Staphylococcus aureus

antimicrobial resistance

biofilmStaphylococcus aureus

biyofilm

antimikrobiyal direnç

Sayı

Makale detayları

Comparison of Biofilm Forming Properties of Staphylococcus aureus Isolates

Yazarlar: Demet Gür Vural | İlknur Bıyık | Elif Gülsüm Torun | Yeliz Tanrıverdi Çaycı | Kemal Bilgin | Asuman Bırıncı

Yayıncı: Duzce University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Sağlık Bilimlerinde Değer Vol.13 Issue 2, pp.245-249

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001289519

pISSN: 2792-0542

Staphylococcus aureus

antimicrobial resistance

biofilmStaphylococcus aureus

biyofilm

antimikrobiyal direnç

Sayı

Yazarlar:

Demet Gür Vural | İlknur Bıyık | Elif Gülsüm Torun | Yeliz Tanrıverdi Çaycı | Kemal Bilgin | Asuman Bırıncı

Yayıncı:

Duzce University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Sağlık Bilimlerinde Değer Vol.13 Issue 2, pp.245-249

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001289519

pISSN:

2792-0542