Evaluation of Some Spermatological Parameters Following Escherichia coli Contamination in Bull Semen

Burcu Esin; Merve Gizem Sezener Kabay; Cumali Kaya; Volkan Enes Ergüden

Makale detayları

Evaluation of Some Spermatological Parameters Following Escherichia coli Contamination in Bull Semen

Yazarlar:

Burcu Esin | Merve Gizem Sezener Kabay | Cumali Kaya | Volkan Enes Ergüden

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 2, pp.164-170

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346917

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 This study aimed to evaluate the effects of extended-spectrum beta-lactamase ESBL producing and non-producing Escherichia coli concentrations on bull semen motility parameters and spermatozoa viability. A total of 50 frozen semen straws from the same Simmental bull were used. All semen straws were thawed in a water bath at 37C and divided into 5 groups of 10 semen samples. Group 1 G1 n10 was the uncontaminated control group. Group 2 G2 n10 was contaminated with 100,000 cfuml and Group 3 G3 n10 was contaminated with E. coli ATCC 25922 at a concentration of 1,000,000 cfuml. Group 4 G4 n10 was contaminated with 100,000 cfuml and Group 5 G5 n10 was contaminated with ESBL producing E. coli BAA-196 at a concentration of 1,000,000 cfuml. In the study, progressive motility, motility and percentages of dead-live spermatozoa in semen samples of these groups were analyzed over time. Significant decreases in spermatozoa motility and viability were observed and the most significant effects were seen in groups G4 and G5 contaminated with high concentrations of ESBL-producing E. coli p0.05. It was determined that the effect of bacterial contamination on spermatological parameters was dose-dependent, with higher concentrations causing more rapid and severe deterioration in semen quality. These findings highlight the role of bacterial contamination, especially with resistant strains, in reducing semen quality and draw attention to the importance of microbial contamination in artificial insemination practices. Further research is needed to explore alternative methods to control contamination in reproductive technologies and combat antibiotic resistance.

 This study aimed to evaluate the effects of extended-spectrum beta-lactamase ESBL producing and non-producing Escherichia coli concentrations on bull semen motility parameters and spermatozoa viability. A total of 50 frozen semen straws from the same Simmental bull were used. All semen straws were thawed in a water bath at 37C and divided into 5 groups of 10 semen samples. Group 1 G1 n10 was the uncontaminated control group. Group 2 G2 n10 was contaminated with 100,000 cfuml and Group 3 G3 n10 was contaminated with E. coli ATCC 25922 at a concentration of 1,000,000 cfuml. Group 4 G4 n10 was contaminated with 100,000 cfuml and Group 5 G5 n10 was contaminated with ESBL producing E. coli BAA-196 at a concentration of 1,000,000 cfuml. In the study, progressive motility, motility and percentages of dead-live spermatozoa in semen samples of these groups were analyzed over time. Significant decreases in spermatozoa motility and viability were observed and the most significant effects were seen in groups G4 and G5 contaminated with high concentrations of ESBL-producing E. coli p0.05. It was determined that the effect of bacterial contamination on spermatological parameters was dose-dependent, with higher concentrations causing more rapid and severe deterioration in semen quality. These findings highlight the role of bacterial contamination, especially with resistant strains, in reducing semen quality and draw attention to the importance of microbial contamination in artificial insemination practices. Further research is needed to explore alternative methods to control contamination in reproductive technologies and combat antibiotic resistance.

 This study aimed to evaluate the effects of extended-spectrum beta-lactamase ESBL producing and non-producing Escherichia coli concentrations on bull semen motility parameters and spermatozoa viability. A total of 50 frozen semen straws from the same Simmental bull were used. All semen straws were thawed in a water bath at 37C and divided into 5 groups of 10 semen samples. Group 1 G1 n10 was the uncontaminated control group. Group 2 G2 n10 was contaminated with 100,000 cfuml and Group 3 G3 n10 was contaminated with E. coli ATCC 25922 at a concentration of 1,000,000 cfuml. Group 4 G4 n10 was contaminated with 100,000 cfuml and Group 5 G5 n10 was contaminated with ESBL producing E. coli BAA-196 at a concentration of 1,000,000 cfuml. In the study, progressive motility, motility and percentages of dead-live spermatozoa in semen samples of these groups were analyzed over time. Significant decreases in spermatozoa motility and viability were observed and the most significant effects were seen in groups G4 and G5 contaminated with high concentrations of ESBL-producing E. coli p0.05. It was determined that the effect of bacterial contamination on spermatological parameters was dose-dependent, with higher concentrations causing more rapid and severe deterioration in semen quality. These findings highlight the role of bacterial contamination, especially with resistant strains, in reducing semen quality and draw attention to the importance of microbial contamination in artificial insemination practices. Further research is needed to explore alternative methods to control contamination in reproductive technologies and combat antibiotic resistance.

 Bu çalışma, genişlemiş spektrumlu beta laktamaz ESBL üreten ve üretmeyen Escherichia coli konsantrasyonlarının boğa sperma motilite parametreleri ve spermatozoa canlılığı üzerindeki etkilerini değerlendirmeyi amaçlamıştır. Aynı Simental boğasından toplam 50 adet donmuş sperma payeti kullanıldı. Tüm sperma payetleri 37Cde su banyosunda çözdürülerek, her biri 10 sperma örneğinden oluşan 5 gruba ayrıldı. Grup 1 G1 n10, kontamine edilmeyen kontrol grubunu oluşturdu. Grup 2 G2 n10, 100.000 cfuml ve Grup 3 G3 n10, 1.000.000 cfuml konsantrasyonunda E. coli ATCC 25922 ile, Grup 4 G4 n10, 100.000 cfuml konsantrasyonda ve Grup 5 G5 n10, 1.000.000 cfuml konsantrasyonda ESBL üreten E. coli BAA-196 ile kontamine edilen grupları oluşturuldu. Çalışmada bu gruplara ait sperma örneklerinde progresif motilite, motilite ve ölü-canlı spermatozoa yüzdelerinin zaman içinde değişimi analiz edildi. Spermatozoa motilitesi ve canlılığında önemli azalmalar gözlemlendi ve en belirgin etkiler, yüksek ESBL üreten E. coli konsantrasyonlarıyla kontamine olan gruplarda G4 ve G5 görüldü p0.05. Bakteriyel kontaminasyonun sperma parametreleri üzerindeki etkisinin doza bağlı olduğu ve daha yüksek konsantrasyonların sperma kalitesinde daha hızlı ve ciddi bozulmaya neden olduğu belirlendi. Bu bulgular, özellikle dirençli suşlarla bakteriyel kontaminasyonun sperma kalitesini azaltmadaki rolünü vurgulamakta ve suni tohumlama uygulamalarında mikrobiyal kontaminasyonun önemine dikkat çekmektedir. Reprodüktif teknolojilerde kontaminasyonu kontrol etmede ve antibiyotik direnciyle mücadele etmeyi sağlamada alternatif yöntemleri keşfetmek için daha fazla araştırmaya ihtiyaç vardır.

Makale detayları

Evaluation of Some Spermatological Parameters Following Escherichia coli Contamination in Bull Semen

Yazarlar:

Burcu Esin | Merve Gizem Sezener Kabay | Cumali Kaya | Volkan Enes Ergüden

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 2, pp.164-170

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346917

pISSN:

1308-1594

Bull

Contamination

Escherichia coli

Sperm parametersBoğa

Kontaminasyon

Escherichia coli

Sperm parametreleri

Sayı

Makale detayları

Yazarlar: Burcu Esin | Merve Gizem Sezener Kabay | Cumali Kaya | Volkan Enes Ergüden

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Dölerme ve Suni Tohumlama

Dergi: Kocatepe Veteriner Dergisi Vol.18 Issue 2, pp.164-170

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346917

pISSN: 1308-1594

Sayı

Yazarlar:

Burcu Esin | Merve Gizem Sezener Kabay | Cumali Kaya | Volkan Enes Ergüden

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Kocatepe Veteriner Dergisi Vol.18 Issue 2, pp.164-170

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346917

pISSN:

1308-1594