Investigation of the Effects of Chrysin Against Azithromycin-Induced Heart Damage in Rats

Serpil Aygörmez; Elif Dalkılınç; Nurhan Akaras; Şaban Maraşlı

Makale detayları

Investigation of the Effects of Chrysin Against Azithromycin-Induced Heart Damage in Rats

Yazarlar:

Serpil Aygörmez | Elif Dalkılınç | Nurhan Akaras | Şaban Maraşlı

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Veterinary Sciences and Practices Vol.20 Issue 2, pp.71-77

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357274

pISSN:

2822-3608

Anahtar kelimeler:

Özet:

 Azithromycin AZM is macrolide antibiotic used to treat infections of the upper and lower respiratory tract. In addition to its therapeutic effects, it has adverse effects such as cardiac and oxidative damage. Chrysin CHR, which is found in propolis and various plants, is a natural flavonoid known for its antioxidant properties. In this study, we investigated the protective effect of CHR against cardiac damage caused by AZM, a broad-spectrum antibiotic. For this purpose, twenty-eight female rats were divided into four groups: Control, CHR, AZM, AZMCHR. AZM 200 mgkg and CHR 50 mgkg were administered orally once daily for seven days. Cardiac markers and oxidative stress parameters were analyzed to determine heart tissue damage. Histopathological analyses were performed to detect tissue damage and structural changes. According to the data obtained from these analyses, AZM increased lactate dehydrogenase LDH and creatine kinase-myocardial band CK-MB activities and cardiac troponin-I cTn-I levels in the heart tissue. AZM toxication significantly increased malondialdehyde MDA levels while reducing the activities of antioxidant enzymes such as superoxide dismutase SOD, glutathione peroxidase GPx, catalase CAT activities and glutathione GSH levels. AZMCHR treatment decreased cardiac tissue cardiac markers LDH, CK-MB, and cTn-I. In addition, CHR treatment together with AZM decreased MDA levels and increased GSH levels and GPx, SOD, and CAT activities. When the findings were evaluated together, it was determined that AZM caused heart damage by increasing cardiac markers and oxidative stress, while CHR supplementation reduced the damage by bringing these parameters closer to normal.

 Azithromycin AZM is macrolide antibiotic used to treat infections of the upper and lower respiratory tract. In addition to its therapeutic effects, it has adverse effects such as cardiac and oxidative damage. Chrysin CHR, which is found in propolis and various plants, is a natural flavonoid known for its antioxidant properties. In this study, we investigated the protective effect of CHR against cardiac damage caused by AZM, a broad-spectrum antibiotic. For this purpose, twenty-eight female rats were divided into four groups: Control, CHR, AZM, AZMCHR. AZM 200 mgkg and CHR 50 mgkg were administered orally once daily for seven days. Cardiac markers and oxidative stress parameters were analyzed to determine heart tissue damage. Histopathological analyses were performed to detect tissue damage and structural changes. According to the data obtained from these analyses, AZM increased lactate dehydrogenase LDH and creatine kinase-myocardial band CK-MB activities and cardiac troponin-I cTn-I levels in the heart tissue. AZM toxication significantly increased malondialdehyde MDA levels while reducing the activities of antioxidant enzymes such as superoxide dismutase SOD, glutathione peroxidase GPx, catalase CAT activities and glutathione GSH levels. AZMCHR treatment decreased cardiac tissue cardiac markers LDH, CK-MB, and cTn-I. In addition, CHR treatment together with AZM decreased MDA levels and increased GSH levels and GPx, SOD, and CAT activities. When the findings were evaluated together, it was determined that AZM caused heart damage by increasing cardiac markers and oxidative stress, while CHR supplementation reduced the damage by bringing these parameters closer to normal.

 Azithromycin AZM is macrolide antibiotic used to treat infections of the upper and lower respiratory tract. In addition to its therapeutic effects, it has adverse effects such as cardiac and oxidative damage. Chrysin CHR, which is found in propolis and various plants, is a natural flavonoid known for its antioxidant properties. In this study, we investigated the protective effect of CHR against cardiac damage caused by AZM, a broad-spectrum antibiotic. For this purpose, twenty-eight female rats were divided into four groups: Control, CHR, AZM, AZMCHR. AZM 200 mgkg and CHR 50 mgkg were administered orally once daily for seven days. Cardiac markers and oxidative stress parameters were analyzed to determine heart tissue damage. Histopathological analyses were performed to detect tissue damage and structural changes. According to the data obtained from these analyses, AZM increased lactate dehydrogenase LDH and creatine kinase-myocardial band CK-MB activities and cardiac troponin-I cTn-I levels in the heart tissue. AZM toxication significantly increased malondialdehyde MDA levels while reducing the activities of antioxidant enzymes such as superoxide dismutase SOD, glutathione peroxidase GPx, catalase CAT activities and glutathione GSH levels. AZMCHR treatment decreased cardiac tissue cardiac markers LDH, CK-MB, and cTn-I. In addition, CHR treatment together with AZM decreased MDA levels and increased GSH levels and GPx, SOD, and CAT activities. When the findings were evaluated together, it was determined that AZM caused heart damage by increasing cardiac markers and oxidative stress, while CHR supplementation reduced the damage by bringing these parameters closer to normal.

 Azitromisin AZM, üst ve alt solunum yolu enfeksiyonlarının tedavisinde kullanılan bir makrolid antibiyotiktir. Terapötik etkilerinin yanında kardiyak ve oksidatif hasar gibi olumsuz etkilere sahiptir. Propoliste ve çeşitli bitkilerde bulunan Krisin CHR, antioksidan özelliğiyle bilinen doğal bir flavonoiddir. Bu çalışmada, geniş spektrumlu bir antibiyotik olan AZMnin neden olduğu kalp hasarına karşı CHRnin koruyucu etkisi araştırıldı. Bu amaçla, yirmi sekiz dişi sıçan Kontrol, CHR, AZM, AZMCHR olmak üzere dört gruba ayrıldı. AZM 200 mgkg ve CHR 50 mgkg yedi gün boyunca günde bir kez oral yoldan uygulandı. Kalp dokusunda hasarı belirlemek için kardiyak belirteçler ve oksidatif stres parametreleri analiz edildi. Doku hasarını ve yapısal değişiklikleri tespit etmek için histopatolojik analizler yapıldı. Bu analizler sonucunda elde edilen verilere göre AZM, kalp dokusunda laktat dehidrogenaz LDH, kreatin kinaz-miyokardiyal bant CK-MB aktiviteleri ve kardiyak troponin-I cTn-I seviyesini artırdı. AZM toksikasyonu, süperoksit dismutaz SOD, glutatyon peroksidaz GPx, katalaz CAT aktiviteleri ve glutatyon GSH seviyeleri gibi antioksidan enzimlerin aktivitelerini azaltırken, malondialdehit MDA seviyelerini önemli ölçüde artırmıştır. AZMCHR tedavisinin kalp dokusu kardiyak belirteçlerinde LDH, CK-MB, cTn-I azalma gösterdiği tespit edildi. Ayrıca, CHR tedavisinin AZM ile birlikte uygulanması MDA düzeyini düşürmüş ve GSH düzeyini ve GPx, SOD ve CAT aktivitelerini artırmıştır. Elde edilen bulgular birlikte değerlendirildiğinde, AZMnin kardiyak belirteçleri ve oksidatif stresi artırarak kalp hasarına neden olduğu, CHR destekleyici tedavisinin ise bu parametreleri normale yakınlaştırarak hasarı azalttığı tespit edildi.

Makale detayları

Investigation of the Effects of Chrysin Against Azithromycin-Induced Heart Damage in Rats

Yazarlar:

Serpil Aygörmez | Elif Dalkılınç | Nurhan Akaras | Şaban Maraşlı

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Veterinary Sciences and Practices Vol.20 Issue 2, pp.71-77

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357274

pISSN:

2822-3608

Azithromycin

Chrysin

Heart

Oxidative stress

RatAzitromisin

Chrysin

Kalp

Oksidatif stres

Rat

Sayı

Makale detayları

Yazarlar: Serpil Aygörmez | Elif Dalkılınç | Nurhan Akaras | Şaban Maraşlı

Yayıncı: Ataturk University

Ana Başlıklar: Veteriner Biyokimya

Dergi: Veterinary Sciences and Practices Vol.20 Issue 2, pp.71-77

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357274

pISSN: 2822-3608

Sayı

Yazarlar:

Serpil Aygörmez | Elif Dalkılınç | Nurhan Akaras | Şaban Maraşlı

Yayıncı:

Ataturk University

Ana Başlıklar:

Veteriner Biyokimya

Dergi:

Veterinary Sciences and Practices Vol.20 Issue 2, pp.71-77

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357274

pISSN:

2822-3608