Intracellular Levels of IL-10 and STAT3 in Patients with Chronic Lymphocytic Leukemia

Ozden Ozcan; Yusuf Metin; Suzan Çınar; Gunnur Deniz

Makale detayları

Intracellular Levels of IL-10 and STAT3 in Patients with Chronic Lymphocytic Leukemia

Yazarlar:

Ozden Ozcan | Yusuf Metin | Suzan Çınar | Gunnur Deniz

Yayıncı:

Marmara University

Ana Başlıklar:

Health Care Administration

Dergi:

Clinical and Experimental Health Sciences Vol.13 Issue 1, pp.99-104

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001286316

pISSN:

2459-1459

Anahtar kelimeler:

Özet:

 Objective: Chronic Lymphocytic Leukemia CLL is characterized by the accumulation of CD5CD19 B cells in the bone marrow and peripheral blood. Recent studies indicated that expression of IL-10, AID and mir-155 which are regulated by STAT3 are increased in CLL patients. CD5CD19 regulator B Breg cells secrete IL-10 and suppress the immune system. While the CLL cells show similar immunophenotypic properties to Breg cells, they are also thought to be functionally similar. In this study, STAT3 and IL-10 levels of CLL patients were investigated. Methods: Peripheral blood samples obtained from patients n:24 and healthy controls n:14. Peripheral blood mononuclear cells were cultured for 48 hours in the presence and absence of CpG for IL-10 expression and cultured with and without PMA for STAT3 expression. IL-10 and STAT3 expression were analyzed with anti-CD5, anti-CD19, anti-CD38, anti-STAT3 and anti-IL-10 monoclonal antibodies by using flow cytometry. Results: Compared to healthy subjects, increased IL-10, IL-10CD19, STAT3CD19 were obtained in lymphocyte population of patients. Increased IL-10 was showed CD19 B cells of CLL patients. Our results showed that IL-10 levels had no significant difference between CD5CD19 cells, whereas STAT3 levels were found lower in patient compared to healthy controls. Conclusion: These results made us thought that the levels of IL-10 and STAT3 expression in CLL B cells is clearly different from normal B lymphocytes might have a role in the biology of CLL. It is believed that the presented data will contribute to the studies that scrutinize the similarity of CLL cells to Breg cells.

 Objective: Chronic Lymphocytic Leukemia CLL is characterized by the accumulation of CD5CD19 B cells in the bone marrow and peripheral blood. Recent studies indicated that expression of IL-10, AID and mir-155 which are regulated by STAT3 are increased in CLL patients. CD5CD19 regulator B Breg cells secrete IL-10 and suppress the immune system. While the CLL cells show similar immunophenotypic properties to Breg cells, they are also thought to be functionally similar. In this study, STAT3 and IL-10 levels of CLL patients were investigated. Methods: Peripheral blood samples obtained from patients n:24 and healthy controls n:14. Peripheral blood mononuclear cells were cultured for 48 hours in the presence and absence of CpG for IL-10 expression and cultured with and without PMA for STAT3 expression. IL-10 and STAT3 expression were analyzed with anti-CD5, anti-CD19, anti-CD38, anti-STAT3 and anti-IL-10 monoclonal antibodies by using flow cytometry. Results: Compared to healthy subjects, increased IL-10, IL-10CD19, STAT3CD19 were obtained in lymphocyte population of patients. Increased IL-10 was showed CD19 B cells of CLL patients. Our results showed that IL-10 levels had no significant difference between CD5CD19 cells, whereas STAT3 levels were found lower in patient compared to healthy controls. Conclusion: These results made us thought that the levels of IL-10 and STAT3 expression in CLL B cells is clearly different from normal B lymphocytes might have a role in the biology of CLL. It is believed that the presented data will contribute to the studies that scrutinize the similarity of CLL cells to Breg cells.

 Objective: Chronic Lymphocytic Leukemia CLL is characterized by the accumulation of CD5CD19 B cells in the bone marrow and peripheral blood. Recent studies indicated that expression of IL-10, AID and mir-155 which are regulated by STAT3 are increased in CLL patients. CD5CD19 regulator B Breg cells secrete IL-10 and suppress the immune system. While the CLL cells show similar immunophenotypic properties to Breg cells, they are also thought to be functionally similar. In this study, STAT3 and IL-10 levels of CLL patients were investigated. Methods: Peripheral blood samples obtained from patients n:24 and healthy controls n:14. Peripheral blood mononuclear cells were cultured for 48 hours in the presence and absence of CpG for IL-10 expression and cultured with and without PMA for STAT3 expression. IL-10 and STAT3 expression were analyzed with anti-CD5, anti-CD19, anti-CD38, anti-STAT3 and anti-IL-10 monoclonal antibodies by using flow cytometry. Results: Compared to healthy subjects, increased IL-10, IL-10CD19, STAT3CD19 were obtained in lymphocyte population of patients. Increased IL-10 was showed CD19 B cells of CLL patients. Our results showed that IL-10 levels had no significant difference between CD5CD19 cells, whereas STAT3 levels were found lower in patient compared to healthy controls. Conclusion: These results made us thought that the levels of IL-10 and STAT3 expression in CLL B cells is clearly different from normal B lymphocytes might have a role in the biology of CLL. It is believed that the presented data will contribute to the studies that scrutinize the similarity of CLL cells to Breg cells.

 Objective: Chronic Lymphocytic Leukemia CLL is characterized by the accumulation of CD5CD19 B cells in the bone marrow and peripheral blood. Recent studies indicated that expression of IL-10, AID and mir-155 which are regulated by STAT3 are increased in CLL patients. CD5CD19 regulator B Breg cells secrete IL-10 and suppress the immune system. While the CLL cells show similar immunophenotypic properties to Breg cells, they are also thought to be functionally similar. In this study, STAT3 and IL-10 levels of CLL patients were investigated. Methods: Peripheral blood samples obtained from patients n:24 and healthy controls n:14. Peripheral blood mononuclear cells were cultured for 48 hours in the presence and absence of CpG for IL-10 expression and cultured with and without PMA for STAT3 expression. IL-10 and STAT3 expression were analyzed with anti-CD5, anti-CD19, anti-CD38, anti-STAT3 and anti-IL-10 monoclonal antibodies by using flow cytometry. Results: Compared to healthy subjects, increased IL-10, IL-10CD19, STAT3CD19 were obtained in lymphocyte population of patients. Increased IL-10 was showed CD19 B cells of CLL patients. Our results showed that IL-10 levels had no significant difference between CD5CD19 cells, whereas STAT3 levels were found lower in patient compared to healthy controls. Conclusion: These results made us thought that the levels of IL-10 and STAT3 expression in CLL B cells is clearly different from normal B lymphocytes might have a role in the biology of CLL. It is believed that the presented data will contribute to the studies that scrutinize the similarity of CLL cells to Breg cells.

Makale detayları

Intracellular Levels of IL-10 and STAT3 in Patients with Chronic Lymphocytic Leukemia

Yazarlar: Ozden Ozcan | Yusuf Metin | Suzan Çınar | Gunnur Deniz

Yayıncı: Marmara University

Ana Başlıklar: Health Care Administration

Dergi: Clinical and Experimental Health Sciences Vol.13 Issue 1, pp.99-104

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001286316

pISSN: 2459-1459

CLL

IL-10

STAT3

Breg

flow cytometry

Sayı

Yazarlar:

Ozden Ozcan | Yusuf Metin | Suzan Çınar | Gunnur Deniz

Yayıncı:

Marmara University

Ana Başlıklar:

Health Care Administration

Dergi:

Clinical and Experimental Health Sciences Vol.13 Issue 1, pp.99-104

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001286316

pISSN:

2459-1459