Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-L- histidine/alginate microbeads by platelet-rich plasma

Gökhan DURUKSU; Selen POLAT; Leyla KAYİŞ; Nur EKİMCİ GÜRCAN; Gülçin GACAR; Yusufhan YAZIR

Makale detayları

Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-L- histidine/alginate microbeads by platelet-rich plasma

Yazarlar:

Gökhan DURUKSU | Selen POLAT | Leyla KAYİŞ | Nur EKİMCİ GÜRCAN | Gülçin GACAR | Yusufhan YAZIR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.297-306

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811207

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 


                        Type 1 diabetes is clinically characterized as the loss of control of glucose homeostasis due to the reduced number of insulinproducing
cells. Long-term glycemic control after implantation could be maintained by preserving the cell viability and tissue-specific
functions during the process of microencapsulation. In this study, alginate solution was supplemented with platelet-rich plasma (PRP)
to improve the viability and preserve the cell functions during the encapsulation of a beta cell line (BRIN-BD11). Cell viability was
assessed and insulin secretion and insulin stimulation index were evaluated. The polymerization of alginate with PRP enhanced the
viability up to 61% in the alginate microbeads. PRP supplementation to the alginate composition not only increased the number of
viable cells by 1.95-fold, but the insulin secretion also improved by about 66%. The stimulation index, however, was not affected by the
PRP supplementation.
                    

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Makale detayları

Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-L- histidine/alginate microbeads by platelet-rich plasma

Yazarlar: Gökhan DURUKSU | Selen POLAT | Leyla KAYİŞ | Nur EKİMCİ GÜRCAN | Gülçin GACAR | Yusufhan YAZIR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.297-306

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811207

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Gökhan DURUKSU | Selen POLAT | Leyla KAYİŞ | Nur EKİMCİ GÜRCAN | Gülçin GACAR | Yusufhan YAZIR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 4, pp.297-306

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811207

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152