In Vitro Direct Shoot Regeneration of Kaman 1 Walnut Variety and Kaman 5 Walnut Genotype (Juglans regia L.)

Ebru Şirin; Sevil Sağlam Yılmaz; Kenan Yıldız

Makale detayları

In Vitro Direct Shoot Regeneration of Kaman 1 Walnut Variety and Kaman 5 Walnut Genotype (Juglans regia L.)

Yazarlar:

Ebru Şirin | Sevil Sağlam Yılmaz | Kenan Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.8 Issue 5, pp.646-652

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358068

pISSN:

2618-6578

Anahtar kelimeler:

Özet:

 In this study, a simple and effective clonal micropropagation protocol was developed from axillary buds of Kaman 1 walnut variety and Kaman 5 walnut genotype. For surface sterilization of explants, they were first treated in 70 ethanol for 1 minute and then treated with 0.2 HgCl2 containing two drops of Tween 20 for 5 minutes. To prevent browning of the explants, 100 mgl Ascorbic Acid, 100 mgl Citric Acid and 100 mgl Ascorbic Acid100 mgl Citric Acid were added to the MS nutrient medium. In addition, explants were subcultured at 3, 24 and 48 hour intervals. It was observed that the most effective treatment in both Kaman 1 walnut variety and Kaman 5 walnut genotype was 100 mgl AA  100 mgl SA  48 hours subculture. Within the scope of the shoot regeneration study, six different combinations of 0.5, 1.0 and 1.5 mgl doses of BAP and 0.5 and 1.0 mgl doses of IBA were tried. The highest average number of shoots per explant 2.67 was obtained from the Kaman 1 variety on MS medium supplemented with 0.5 mgl BAP and 1.0 mgl IBA. On the other hand, in the medium containing 0.5 mgl BAP and 0.5 mgl IBA, the average number of shoots was 2.00 for the Kaman 1 cultivar and 0.67 for the Kaman 5 genotype. After the shoots were kept in a solution containing 4 mgl IBA for 10 minutes, they were planted in pots containing a peat: perlite 1:1 mixture, but rooting couldnt be achieved from the shoots.

 In this study, a simple and effective clonal micropropagation protocol was developed from axillary buds of Kaman 1 walnut variety and Kaman 5 walnut genotype. For surface sterilization of explants, they were first treated in 70 ethanol for 1 minute and then treated with 0.2 HgCl2 containing two drops of Tween 20 for 5 minutes. To prevent browning of the explants, 100 mgl Ascorbic Acid, 100 mgl Citric Acid and 100 mgl Ascorbic Acid100 mgl Citric Acid were added to the MS nutrient medium. In addition, explants were subcultured at 3, 24 and 48 hour intervals. It was observed that the most effective treatment in both Kaman 1 walnut variety and Kaman 5 walnut genotype was 100 mgl AA  100 mgl SA  48 hours subculture. Within the scope of the shoot regeneration study, six different combinations of 0.5, 1.0 and 1.5 mgl doses of BAP and 0.5 and 1.0 mgl doses of IBA were tried. The highest average number of shoots per explant 2.67 was obtained from the Kaman 1 variety on MS medium supplemented with 0.5 mgl BAP and 1.0 mgl IBA. On the other hand, in the medium containing 0.5 mgl BAP and 0.5 mgl IBA, the average number of shoots was 2.00 for the Kaman 1 cultivar and 0.67 for the Kaman 5 genotype. After the shoots were kept in a solution containing 4 mgl IBA for 10 minutes, they were planted in pots containing a peat: perlite 1:1 mixture, but rooting couldnt be achieved from the shoots.

 In this study, a simple and effective clonal micropropagation protocol was developed from axillary buds of Kaman 1 walnut variety and Kaman 5 walnut genotype. For surface sterilization of explants, they were first treated in 70 ethanol for 1 minute and then treated with 0.2 HgCl2 containing two drops of Tween 20 for 5 minutes. To prevent browning of the explants, 100 mgl Ascorbic Acid, 100 mgl Citric Acid and 100 mgl Ascorbic Acid100 mgl Citric Acid were added to the MS nutrient medium. In addition, explants were subcultured at 3, 24 and 48 hour intervals. It was observed that the most effective treatment in both Kaman 1 walnut variety and Kaman 5 walnut genotype was 100 mgl AA  100 mgl SA  48 hours subculture. Within the scope of the shoot regeneration study, six different combinations of 0.5, 1.0 and 1.5 mgl doses of BAP and 0.5 and 1.0 mgl doses of IBA were tried. The highest average number of shoots per explant 2.67 was obtained from the Kaman 1 variety on MS medium supplemented with 0.5 mgl BAP and 1.0 mgl IBA. On the other hand, in the medium containing 0.5 mgl BAP and 0.5 mgl IBA, the average number of shoots was 2.00 for the Kaman 1 cultivar and 0.67 for the Kaman 5 genotype. After the shoots were kept in a solution containing 4 mgl IBA for 10 minutes, they were planted in pots containing a peat: perlite 1:1 mixture, but rooting couldnt be achieved from the shoots.

 In this study, a simple and effective clonal micropropagation protocol was developed from axillary buds of Kaman 1 walnut variety and Kaman 5 walnut genotype. For surface sterilization of explants, they were first treated in 70 ethanol for 1 minute and then treated with 0.2 HgCl2 containing two drops of Tween 20 for 5 minutes. To prevent browning of the explants, 100 mgl Ascorbic Acid, 100 mgl Citric Acid and 100 mgl Ascorbic Acid100 mgl Citric Acid were added to the MS nutrient medium. In addition, explants were subcultured at 3, 24 and 48 hour intervals. It was observed that the most effective treatment in both Kaman 1 walnut variety and Kaman 5 walnut genotype was 100 mgl AA  100 mgl SA  48 hours subculture. Within the scope of the shoot regeneration study, six different combinations of 0.5, 1.0 and 1.5 mgl doses of BAP and 0.5 and 1.0 mgl doses of IBA were tried. The highest average number of shoots per explant 2.67 was obtained from the Kaman 1 variety on MS medium supplemented with 0.5 mgl BAP and 1.0 mgl IBA. On the other hand, in the medium containing 0.5 mgl BAP and 0.5 mgl IBA, the average number of shoots was 2.00 for the Kaman 1 cultivar and 0.67 for the Kaman 5 genotype. After the shoots were kept in a solution containing 4 mgl IBA for 10 minutes, they were planted in pots containing a peat: perlite 1:1 mixture, but rooting couldnt be achieved from the shoots.

Makale detayları

In Vitro Direct Shoot Regeneration of Kaman 1 Walnut Variety and Kaman 5 Walnut Genotype (Juglans regia L.)

Yazarlar:

Ebru Şirin | Sevil Sağlam Yılmaz | Kenan Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.8 Issue 5, pp.646-652

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358068

pISSN:

2618-6578

Walnut

İn vitro

Tissue culture

Micropropagation

Antioxidant

SubcultureWalnut

İn vitro

Tissue culture

Micropropagation

Antioxidant

Subculture

Sayı

Makale detayları

Yazarlar: Ebru Şirin | Sevil Sağlam Yılmaz | Kenan Yıldız

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Black Sea Journal of Agriculture Vol.8 Issue 5, pp.646-652

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001358068

pISSN: 2618-6578

Sayı

Yazarlar:

Ebru Şirin | Sevil Sağlam Yılmaz | Kenan Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.8 Issue 5, pp.646-652

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001358068

pISSN:

2618-6578