The Potential of Aqueous Two-Phase (Ethanol-Salt Based) Systems in the Extraction of Bioactive Compounds: Pistachio (Pistacia vera L.) Wastes as a Model Structure

Mehmet Şükrü Karakuş

Makale detayları

The Potential of Aqueous Two-Phase (Ethanol-Salt Based) Systems in the Extraction of Bioactive Compounds: Pistachio (Pistacia vera L.) Wastes as a Model Structure

Yazarlar:

Mehmet Şükrü Karakuş

Yayıncı:

Igdir University

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.543-555

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345235

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 This study investigates the characterization of pistachio waste and evaluates the effectiveness of an alcoholsalt-based aqueous two-phase extraction ATPS method for purifying bioactive compounds in this material by comparing with the conventional method. In the initial stage, the physicochemical properties of pistachio wastes moisture content, total ash, oil content, and color values were analyzed. Fourier transform infrared spectroscopy FTIR confirmed the characteristic structure of pistachio wastes. In addition, the mineral content of the wastes was determined by Inductively Coupled Plasma-Optical Emission Spectrometry ICP-OES. In the second stage, the composition of ATPS was selected. The ratio of ethanol to ammonium sulfate used in the composition of ATPS was based on the total phenolic content variable. The ATPS-3 point with the highest phenolic content was identified as the optimal point. The ethanol ratio was 28, while the ammonium sulfate ratio was 18. The values for the volume ratio R and partition coefficient K under optimum conditions were determined as 1.56 and 10.00, respectively. Furthermore, the optimum ATPS point was compared with the traditional extraction regarding total phenolic substances, total flavonoids, total hydrolyzed tannins, and antioxidant activity values. ATPS extracts demonstrated greater biological activity compared to traditional extraction methods. In conclusion, this innovative extraction method can serve as a viable alternative to traditional extraction techniques.

 This study investigates the characterization of pistachio waste and evaluates the effectiveness of an alcoholsalt-based aqueous two-phase extraction ATPS method for purifying bioactive compounds in this material by comparing with the conventional method. In the initial stage, the physicochemical properties of pistachio wastes moisture content, total ash, oil content, and color values were analyzed. Fourier transform infrared spectroscopy FTIR confirmed the characteristic structure of pistachio wastes. In addition, the mineral content of the wastes was determined by Inductively Coupled Plasma-Optical Emission Spectrometry ICP-OES. In the second stage, the composition of ATPS was selected. The ratio of ethanol to ammonium sulfate used in the composition of ATPS was based on the total phenolic content variable. The ATPS-3 point with the highest phenolic content was identified as the optimal point. The ethanol ratio was 28, while the ammonium sulfate ratio was 18. The values for the volume ratio R and partition coefficient K under optimum conditions were determined as 1.56 and 10.00, respectively. Furthermore, the optimum ATPS point was compared with the traditional extraction regarding total phenolic substances, total flavonoids, total hydrolyzed tannins, and antioxidant activity values. ATPS extracts demonstrated greater biological activity compared to traditional extraction methods. In conclusion, this innovative extraction method can serve as a viable alternative to traditional extraction techniques.

 This study investigates the characterization of pistachio waste and evaluates the effectiveness of an alcoholsalt-based aqueous two-phase extraction ATPS method for purifying bioactive compounds in this material by comparing with the conventional method. In the initial stage, the physicochemical properties of pistachio wastes moisture content, total ash, oil content, and color values were analyzed. Fourier transform infrared spectroscopy FTIR confirmed the characteristic structure of pistachio wastes. In addition, the mineral content of the wastes was determined by Inductively Coupled Plasma-Optical Emission Spectrometry ICP-OES. In the second stage, the composition of ATPS was selected. The ratio of ethanol to ammonium sulfate used in the composition of ATPS was based on the total phenolic content variable. The ATPS-3 point with the highest phenolic content was identified as the optimal point. The ethanol ratio was 28, while the ammonium sulfate ratio was 18. The values for the volume ratio R and partition coefficient K under optimum conditions were determined as 1.56 and 10.00, respectively. Furthermore, the optimum ATPS point was compared with the traditional extraction regarding total phenolic substances, total flavonoids, total hydrolyzed tannins, and antioxidant activity values. ATPS extracts demonstrated greater biological activity compared to traditional extraction methods. In conclusion, this innovative extraction method can serve as a viable alternative to traditional extraction techniques.

 Bu çalışma, fıstık posası atıklarının karakterizasyonunu ve bu materyaldeki biyoaktif bileşikleri saflaştırmak amacıyla alkoltuz bazlı sulu iki fazlı özütleme ATPS yönteminin etkinliğini geleneksel yöntemle kıyaslayarak araştırmaktadır. İlk aşamada, fıstık posasının fizikokimyasal özellikleri nem içeriği, toplam kül, yağ oranı ve renk değerleri belirlenmiştir. Fıstık atıklarının karakteristik yapısı Fourier dönüşümlü kızılötesi spektroskopisi FTIR ile doğrulanmıştır. Ayrıca, atıkların mineral madde içeriği İndüktif Eşleşmiş Plazma-Optik Emisyon Spektrometresi ICP-OES ile belirlenmiştir. İkinci aşamada ise ATPSnin bileşimi belirlenmiştir. ATPSnin bileşiminde kullanılan etanol ve amonyum sülfat oranı toplam fenolik madde değişkeni baz alınarak tespit edilmiştir. En yüksek fenolik maddeye sahip ATPS-3 noktası en uygun nokta olarak seçilmiştir. Bu noktada kullanılan etanol oranı 28 ve amonyum sülfat oranı ise 18 olarak bulunmuştur. Optimum koşullardaki hacim oranı R ve bölüşüm katsayısı K değerleri ise 1.56 ve 10.00 olarak tespit edilmiştir. Optimum ATPS noktası ile geleneksel özütleme toplam fenolik madde, toplam flavonoid, toplam hidrolize tanen ve antioksidan aktivite değerleri açısından karşılaştırılmıştır. ATPS özütlerinin biyolojik aktivite değerleri açısından geleneksel özütlemeye göre daha üstün olduğu bulunmuştur. Çalışma sonunda, bu yenilikçi özütleme yönteminin geleneksel özütleme yöntemlerine bir alternatif olabileceği sonucuna varılabilir.

Makale detayları

The Potential of Aqueous Two-Phase (Ethanol-Salt Based) Systems in the Extraction of Bioactive Compounds: Pistachio (Pistacia vera L.) Wastes as a Model Structure

Yazarlar:

Mehmet Şükrü Karakuş

Yayıncı:

Igdir University

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.543-555

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345235

pISSN:

2536-4618

Pistachio waste

Two phase extraction

FTIR

Flavonoid

TanninFıstık atığı

İki fazlı özütleme

FTIR

Flavonoid

Tanen

Sayı

Makale detayları

Yazarlar: Mehmet Şükrü Karakuş

Yayıncı: Igdir University

Ana Başlıklar: Gıda Mühendisliği

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.543-555

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001345235

pISSN: 2536-4618

Sayı

Yazarlar:

Mehmet Şükrü Karakuş

Yayıncı:

Igdir University

Ana Başlıklar:

Gıda Mühendisliği

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.543-555

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345235

pISSN:

2536-4618