Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması

Ayşegül AKSOY GÖKMEN; Hüsniye KAYALAR; Bayram PEKTAŞ; Selçuk KAYA

Makale detayları

Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması

Yazarlar:

Ayşegül AKSOY GÖKMEN | Hüsniye KAYALAR | Bayram PEKTAŞ | Selçuk KAYA

Yayıncı:

Izmir Katip Celebi University

Dergi:

İzmir Katip Çelebi University Faculty of Health Science Journal 2018 Vol.3 Issue 3, pp.7-10

Belge Tipi:

Makale

Yayın Tarihi:

10/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000483601

Sıklık:

Üç Yıllık

pISSN:

2458-9799

Özet:

 





Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması



 

Özet 


Anahtar
Kelimeler: (3-5 Kelime): Trichomonas vaginalis, nigella sativa, anti-trikomoniyaz etki



 
















Investigation of in vitro Anti-trichomoniasis Effect of Nigella Sativa Oil against Trichomonas vaginalis



 

Abstract 


Objective: Although 5-nitroimidazole derivatives (metronidazole, ornidazole) are being used in the current treatment of trichomoniasis, it has been known the presence of MTZ-resistant T. vaginalis strains for almost 50 years. Therefore, alternative treatment methods are being investigated for a long time for treatment of trichomoniasis. In this study, it was aimed to investigate in vitro activity of Nigella sativa oil against Trichomonas vaginalis. Material and Methods: In vitro testing of Nigella sativa and metronidazole against T. vaginalis was performed in cell culture plate by dilution method. Briefly, T. vaginalis trophozoites were transferred to 24-well plate containing TYM medium. Thereafter, different concentrations of Nigella sativa and metronidazole were added to each well and incubated at 37°C. Findings: At 48th hour of incubation, although motile trophozoites at 250 μg/ml concentration of Nigella sativa oil were observed, immotile trophozoites were detected at 500 μg/ml, 1000 μg/ml and 2000 μl/ml concentrations of Nigella sativa oil. At 72th hour, immotile trophozoites as well as trophozoite lysis were detected in all concentration of Nigella sativa oil, except for 250 μg/ ml concentration. Also at the end of 72th hour for Nigella sativa, MIC was 500 μl/mg while MLK was 1000μl/

mg. Conclusion: In this study, Nigella sativa oil was tested in vitro as an alternative therapeutic agent for treatment of trichomoniasis and it has been found that Nigella sativa oil has a naturally important therapeutic potential for trichomoniasis treatment. In vivo tests and the isolation and identification of compounds responsible for the detected activity are planned for further investigations


Keywords:
(3-5
Words): Trichomonas vaginalis, nigella sativa, anti-trichomoniasis effect




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Makale detayları

Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması

Yazarlar: Ayşegül AKSOY GÖKMEN | Hüsniye KAYALAR | Bayram PEKTAŞ | Selçuk KAYA

Yayıncı: Izmir Katip Celebi University

Dergi: İzmir Katip Çelebi University Faculty of Health Science Journal 2018 Vol.3 Issue 3, pp.7-10

Belge Tipi: Makale

Yayın Tarihi: 10/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000483601

Sıklık: Üç Yıllık

pISSN: 2458-9799

Sayı

Yazarlar:

Ayşegül AKSOY GÖKMEN | Hüsniye KAYALAR | Bayram PEKTAŞ | Selçuk KAYA

Yayıncı:

Izmir Katip Celebi University

Dergi:

İzmir Katip Çelebi University Faculty of Health Science Journal 2018 Vol.3 Issue 3, pp.7-10

Belge Tipi:

Makale

Yayın Tarihi:

10/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000483601

Sıklık:

Üç Yıllık

pISSN:

2458-9799