THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2

Gultekin Akdamar; Ozgur Ceylan; Aysel Ugur

Makale detayları

THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2

Yazarlar:

Gultekin Akdamar | Ozgur Ceylan | Aysel Ugur

Yayıncı:

Muğla Sıtkı Koçman Üniversitesi

Dergi:

Mugla Journal of Science and Technology 2016, pp.7-10

Belge Tipi:

Makale

Yayın Tarihi:

5/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000577034

Sıklık:

İki Yıllık

pISSN:

2149-3596

Özet:

 

Streptomyces griseoflavus OC. 124-2 used in this study was isolated from the field soil of Dalaman Directorate of Agricultural Enterprises Muğla. As a result of phenotypic and molecular characterization, the isolate was identified as Streptomyces griseoflavus and named as OC. 124-2. The fermentation liquid of Streptomyces griseoflavus OC. 124-2 was obtained in optimum fermentation conditions, and then it was filtered and extracted with ethyl acetate 1:1. The extract containing the active compounds was obtained by evaporating the solvent. Biomonitor strains, Chromobacterium violaceum CV026 for the determination of anti-quorum sensing activity (anti-QS), Chromobacterium violaceum CV12472 for the determination of inhibition of violacein pigment production and Pseudomonas aeruginosa PA01 for the determination of anti-swarming activity were used at MIC and sub-MIC concentrations. The anti-quorum sensing and anti-swarming activities could not be detected for the extract. Violacein production was inhibited by 100%, 74.86%, 65.74% and 31.99% at MIC, MIC/2, MIC/4 and MIC/8 concentrations of the extract treatment, respectively. While the detected inhibition of violacein pigment production did not inhibit the bacterial growth, it was revealed that it inhibited the quorum-sensing-regulated signaling systems. Accordingly, it was shown that the active compounds obtained from ethyl acetate extract of OC. 124-2 constituted a non-selective pressure for the growth of drug resistant pathogen bacteria and they may be used as an alternative at treatment of these bacteria.

Makale detayları

THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2

Yazarlar: Gultekin Akdamar | Ozgur Ceylan | Aysel Ugur

Yayıncı: Muğla Sıtkı Koçman Üniversitesi

Dergi: Mugla Journal of Science and Technology 2016, pp.7-10

Belge Tipi: Makale

Yayın Tarihi: 5/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000577034

Sıklık: İki Yıllık

pISSN: 2149-3596

Sayı

Yazarlar:

Gultekin Akdamar | Ozgur Ceylan | Aysel Ugur

Yayıncı:

Muğla Sıtkı Koçman Üniversitesi

Dergi:

Mugla Journal of Science and Technology 2016, pp.7-10

Belge Tipi:

Makale

Yayın Tarihi:

5/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000577034

Sıklık:

İki Yıllık

pISSN:

2149-3596