Neurotoxic effects of bisphenol A on SH-SY5Y  neuroblastoma cells via nitric oxide

Zekiye Tuba Tüylü Küçükkılınç

Makale detayları

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Yazarlar:

Zekiye Tuba Tüylü Küçükkılınç

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.354-359

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347183

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Bisphenol A BPA, 2,2-bis4-hydroxyphenylpropanepolycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA 0.01 μM-100 μM  and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses 0.01 μM-100 μM for 8, 24, 72 hours. Measurements to determine the nitric oxide NO levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups p0.001. A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA 0.01 μM-100 μM for 8 and 72 hours  p0.01, p0.001 respectively. Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

 Bisphenol A BPA, 2,2-bis4-hydroxyphenylpropanepolycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA 0.01 μM-100 μM  and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses 0.01 μM-100 μM for 8, 24, 72 hours. Measurements to determine the nitric oxide NO levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups p0.001. A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA 0.01 μM-100 μM for 8 and 72 hours  p0.01, p0.001 respectively. Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

 Bisphenol A BPA, 2,2-bis4-hydroxyphenylpropanepolycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA 0.01 μM-100 μM  and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses 0.01 μM-100 μM for 8, 24, 72 hours. Measurements to determine the nitric oxide NO levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups p0.001. A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA 0.01 μM-100 μM for 8 and 72 hours  p0.01, p0.001 respectively. Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

 Bisphenol A BPA, 2,2-bis4-hydroxyphenylpropanepolycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA 0.01 μM-100 μM  and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses 0.01 μM-100 μM for 8, 24, 72 hours. Measurements to determine the nitric oxide NO levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups p0.001. A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA 0.01 μM-100 μM for 8 and 72 hours  p0.01, p0.001 respectively. Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

Makale detayları

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Yazarlar:

Zekiye Tuba Tüylü Küçükkılınç

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.354-359

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347183

pISSN:

2630-6344

Bisphenol A

nitric oxide

cytotoxicity

neurotoxicity.

Sayı

Makale detayları

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Yazarlar: Zekiye Tuba Tüylü Küçükkılınç

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Biochemistry

Dergi: Journal of Research in Pharmacy Vol.23 Issue 3, pp.354-359

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347183

pISSN: 2630-6344

Bisphenol A

nitric oxide

cytotoxicity

neurotoxicity.

Sayı

Yazarlar:

Zekiye Tuba Tüylü Küçükkılınç

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.354-359

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347183

pISSN:

2630-6344