The Investigation of the Changes in the Surface Glycoconjugates Using Two Different Spheroid Models of Breast Cancer Cells and Availability Assessment of These Spheroid Models for Rapid Diagnosis

Yosun MATER; Günnur DEMİRCAN

Makale detayları

The Investigation of the Changes in the Surface Glycoconjugates Using Two Different Spheroid Models of Breast Cancer Cells and Availability Assessment of These Spheroid Models for Rapid Diagnosis

Yazarlar:

Yosun MATER | Günnur DEMİRCAN

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.266-271

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000507474

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483

Özet:

 

The importance of early cancer diagnosis has led to development of many different diagnostic methods. In this context, the studies investigating the presence and amount of sugar residues to use as indicators in the identification of cancer cell type have become prominent. In the present study, sialic acids found on the membrane surfaces of ER (+) MCF-7 and ER (-) MDA-MB-231 breast cancer cell lines were labeled using three dimensional (3D) cell culture (Spheroid) model as the closest method to the patient sample, thus its natural environment, among the cell culture methods. These sugar units that play a role in regulation of important immune characteristics such as recognition, binding and metastasis were made visualizable by applying fluorescent-labeled lectins such as FITC-(Wheat Germ Agglutinin) specifically binding to sialic acid units (GlcNAc, Neu5Ac) including particularly ß-GlcNAc and FITC-(Maackia Amurensis-Lectin-1) specifically binding to Galß4GlcNAc type sialic acids. These glycan units were specifically labeled with FITC-(Maackia Amurensis-Lectin-1) and FITC-(Wheat Germ Agglutinin) and radiation intensities were analyzed relatively. The two different breast cancer cell cultures were compared with respect to change in the amounts of sialic acid residues containing α-2,3- and α-2,6 bonds using fluorescent-labeled lectins.
In the present study, we have performed a precise, accurate and rapid determination of the sugar content in the different breast cancer cell surface lines by means of fluorescent-labeled lectins and carried out a relative comparison between the micrographs.

Makale detayları

The Investigation of the Changes in the Surface Glycoconjugates Using Two Different Spheroid Models of Breast Cancer Cells and Availability Assessment of These Spheroid Models for Rapid Diagnosis

Yazarlar: Yosun MATER | Günnur DEMİRCAN

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.266-271

Belge Tipi: Makale

Yayın Tarihi: 4/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000507474

Sıklık: Üç Aylık

eISSN: 1309-5129

pISSN: 1309-4483

Sayı

Yazarlar:

Yosun MATER | Günnur DEMİRCAN

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.266-271

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000507474

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483