Morphological, Molecular Identification and Virulence of Entomopathogenic Fungi Isolated From Dendroctonus micans (Kugelann,1794) (Coleoptera: Curculionidae)

Seda Biryol; Ali Soydinç; Sevda İşık

Makale detayları

Morphological, Molecular Identification and Virulence of Entomopathogenic Fungi Isolated From Dendroctonus micans (Kugelann,1794) (Coleoptera: Curculionidae)

Yazarlar:

Seda Biryol | Ali Soydinç | Sevda İşık

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.28 Issue 2, pp.381-391

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315412

pISSN:

2147-835X

Anahtar kelimeler:

Özet:

 In this study, to determine an effective fungal agent against Dendroctonus micans Kugelann, 1794 Coleoptera: Curculionidae, which causes significant economic losses in forested areas, Picea orientalis L. Link in Artvin between 2021-2022. Dendroctonus micans larvae and adults were collected from the trees, and 18 fungi were isolated from larvae and adult insects. Morphological infection type, colony morphology, spore form and molecular ITS1-5.8S ITS2 gene region characterization determined that the isolates were Metarhizium anisopliae Metschn. Sorokin, 1883 Hypocreales: Clavicipitaceae, M. robertsii, M. pinghaense and Clonostachys rosea Samuels  Rossman, 1999 Hypocreales: Bionectriaceae. Isolates M. robertsii OZM4 and M. pinhaense OZM9 have been isolated from this pest for the first time. As a result of insecticidal activity tests performed on D. micans larvae and adults of 1x107sporeml spore suspension, the larvae, M. anisopliae OZM2, showed a mortality rate of 92 within 7 days and adults mortality was determined 100 at the end of the experiment, and mycosis rates were found to be consistent with mortality rates. These results show that isolates with high virulence are promising in microbial and integrated control applications against important forest pests.

 In this study, to determine an effective fungal agent against Dendroctonus micans Kugelann, 1794 Coleoptera: Curculionidae, which causes significant economic losses in forested areas, Picea orientalis L. Link in Artvin between 2021-2022. Dendroctonus micans larvae and adults were collected from the trees, and 18 fungi were isolated from larvae and adult insects. Morphological infection type, colony morphology, spore form and molecular ITS1-5.8S ITS2 gene region characterization determined that the isolates were Metarhizium anisopliae Metschn. Sorokin, 1883 Hypocreales: Clavicipitaceae, M. robertsii, M. pinghaense and Clonostachys rosea Samuels  Rossman, 1999 Hypocreales: Bionectriaceae. Isolates M. robertsii OZM4 and M. pinhaense OZM9 have been isolated from this pest for the first time. As a result of insecticidal activity tests performed on D. micans larvae and adults of 1x107sporeml spore suspension, the larvae, M. anisopliae OZM2, showed a mortality rate of 92 within 7 days and adults mortality was determined 100 at the end of the experiment, and mycosis rates were found to be consistent with mortality rates. These results show that isolates with high virulence are promising in microbial and integrated control applications against important forest pests.

 In this study, to determine an effective fungal agent against Dendroctonus micans Kugelann, 1794 Coleoptera: Curculionidae, which causes significant economic losses in forested areas, Picea orientalis L. Link in Artvin between 2021-2022. Dendroctonus micans larvae and adults were collected from the trees, and 18 fungi were isolated from larvae and adult insects. Morphological infection type, colony morphology, spore form and molecular ITS1-5.8S ITS2 gene region characterization determined that the isolates were Metarhizium anisopliae Metschn. Sorokin, 1883 Hypocreales: Clavicipitaceae, M. robertsii, M. pinghaense and Clonostachys rosea Samuels  Rossman, 1999 Hypocreales: Bionectriaceae. Isolates M. robertsii OZM4 and M. pinhaense OZM9 have been isolated from this pest for the first time. As a result of insecticidal activity tests performed on D. micans larvae and adults of 1x107sporeml spore suspension, the larvae, M. anisopliae OZM2, showed a mortality rate of 92 within 7 days and adults mortality was determined 100 at the end of the experiment, and mycosis rates were found to be consistent with mortality rates. These results show that isolates with high virulence are promising in microbial and integrated control applications against important forest pests.

 In this study, to determine an effective fungal agent against Dendroctonus micans Kugelann, 1794 Coleoptera: Curculionidae, which causes significant economic losses in forested areas, Picea orientalis L. Link in Artvin between 2021-2022. Dendroctonus micans larvae and adults were collected from the trees, and 18 fungi were isolated from larvae and adult insects. Morphological infection type, colony morphology, spore form and molecular ITS1-5.8S ITS2 gene region characterization determined that the isolates were Metarhizium anisopliae Metschn. Sorokin, 1883 Hypocreales: Clavicipitaceae, M. robertsii, M. pinghaense and Clonostachys rosea Samuels  Rossman, 1999 Hypocreales: Bionectriaceae. Isolates M. robertsii OZM4 and M. pinhaense OZM9 have been isolated from this pest for the first time. As a result of insecticidal activity tests performed on D. micans larvae and adults of 1x107sporeml spore suspension, the larvae, M. anisopliae OZM2, showed a mortality rate of 92 within 7 days and adults mortality was determined 100 at the end of the experiment, and mycosis rates were found to be consistent with mortality rates. These results show that isolates with high virulence are promising in microbial and integrated control applications against important forest pests.

Makale detayları

Morphological, Molecular Identification and Virulence of Entomopathogenic Fungi Isolated From Dendroctonus micans (Kugelann,1794) (Coleoptera: Curculionidae)

Yazarlar:

Seda Biryol | Ali Soydinç | Sevda İşık

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.28 Issue 2, pp.381-391

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315412

pISSN:

2147-835X

Dendroctonus micans

Entomopathogenic fungus

Metarhizium

Microbiology

Mycology

Sayı

Makale detayları

Morphological, Molecular Identification and Virulence of Entomopathogenic Fungi Isolated From Dendroctonus micans (Kugelann,1794) (Coleoptera: Curculionidae)

Yazarlar: Seda Biryol | Ali Soydinç | Sevda İşık

Yayıncı: Sakarya University

Ana Başlıklar: Structural Biology

Dergi: Sakarya University Journal of Science Vol.28 Issue 2, pp.381-391

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001315412

pISSN: 2147-835X

Dendroctonus micans

Entomopathogenic fungus

Metarhizium

Microbiology

Mycology

Sayı

Yazarlar:

Seda Biryol | Ali Soydinç | Sevda İşık

Yayıncı:

Sakarya University

Ana Başlıklar:

Structural Biology

Dergi:

Sakarya University Journal of Science Vol.28 Issue 2, pp.381-391

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315412

pISSN:

2147-835X