The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model

İnayet GÜNTÜRK; Cevat YAZICI; Sema Kader KÖSE; Fatma DAĞLI; Bilal YÜCEL; Arzu Hanim YAY

Makale detayları

The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model

Yazarlar:

İnayet GÜNTÜRK | Cevat YAZICI | Sema Kader KÖSE | Fatma DAĞLI | Bilal YÜCEL | Arzu Hanim YAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1789-1799

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749813

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/Aim:  Cisplatin  is  a  highly  effective  chemotherapeutic  agent  used  in  the  treatment  of  solid  organ  cancers.  Besides  its  chemotherapeutic effectiveness, cisplatin administration is associated with numerous side effects. Of those, the most clinically significant and common effect is nephrotoxicity. Recent studies reported that oxidative stress and inflammation are probably the most important mechanisms that contribute to the nephrotoxicity. N-acetylcysteine (NAC) is an antioxidant and antiinflammatory agent. In the present study, the effects of NAC on cisplatin-induced nephrotoxicity were investigated. Materials  and  methods:  Rats  were  divided  into  four  groups  each  including  eight  rats:  CONT,  NAC-250,  CP,  and  CP+NAC.  Rats  in  experimental groups were treated intraperitoneally (i.p.) with a single dose of cisplatin (10 mg/kg body weight) and i.p. with NAC (250 mg/kg body weight) for three consecutive days. Nephrotoxicity was determined by plasma BUN and creatinine levels. In tissue samples, myeloperoxidase (MPO), nuclear factor-kappa B (NF-kB), high mobility group box-1 (HMGB-1), total oxidant status (TOS), and total antioxidant status (TAS) levels were measured. Kidneys were analyzed histopathologically as well.Results: It was revealed that cisplatin was not effective on MPO, HMGB-1 and NF-kB levels but did increase TOS levels and decrease TAS  levels  in  tissue  samples.  Interestingly,  NAC  elevated  MPO  and  HMGB-1  levels  significantly.  Nevertheless,  NAC  ameliorated  histological and functional changes in kidney tissues.Conclusion:  It  is  suggested  that  inflammation  has  a  limited  effect  on  cisplatin  nephrotoxicity  in  this  experimental  design,  and,  as  reflected by decreased BUN and creatinine levels, NAC can be used as an additional therapeutic agent in standard cisplatin treatment protocols.

Makale detayları

The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model

Yazarlar: İnayet GÜNTÜRK | Cevat YAZICI | Sema Kader KÖSE | Fatma DAĞLI | Bilal YÜCEL | Arzu Hanim YAY

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1789-1799

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000749813

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

İnayet GÜNTÜRK | Cevat YAZICI | Sema Kader KÖSE | Fatma DAĞLI | Bilal YÜCEL | Arzu Hanim YAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 6, pp.1789-1799

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000749813

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144