Limb remote ischemic postconditioning is effective but also time-course-limited in protecting the brain from I/R injury

Ce XU; Chenju YI; Haidong GUO; Qiangqiang LIU; Li WANG; Hongxia LI

Makale detayları

Limb remote ischemic postconditioning is effective but also time-course-limited in protecting the brain from I/R injury

Yazarlar:

Ce XU | Chenju YI | Haidong GUO | Qiangqiang LIU | Li WANG | Hongxia LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.918-929

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754056

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To determine the effect of limb remote ischemic postconditioning (LIPoC) against cerebral ischemia reperfusion in rats and the relationship between cycle number or remote postconditioning episode duration and the protective function of LIPoC.  Materials and methods: Transient focal ischemia/reperfusion was induced by 90 min of middle cerebral artery occlusion (MCAO) with nylon monofilament and 22 h of reperfusion in male Sprague-Dawley rats. The animals underwent LIPoC consisting of 1, 2, or 3 cycles, with each occlusion or release lasting for 5 min (5/5), 10 min (10/10), or 15 min (15/15) of bilateral femoral artery occlusion/release. Neurological score, infarct volume, water content, blood-brain barrier permeability (BBB), neuronal analysis, immunohistochemical detection, and mitochondrial permeability transition pore opening were measured.  Results: Infarct volume was significantly reduced in the groups with 2 cycles of 15/15, 3 cycles of 10/10, and 2 cycles of 10/10. These 3 groups also had attenuated brain edema, BBB disruption, and apoptosis compared to both the MCAO and sham groups.  Conclusion: This study affirmed the protective effect of LIPoC on cerebral reperfusion injury. Furthermore, it indicated that the accumulative time of limb occlusion/reperfusion might be crucial in remote postconditioning and that LIPoC exerted its maximum protective effect if the accumulative time of limb occlusion/reperfusion lasted from 40 to 60 min.

 

To determine the effect of limb remote ischemic postconditioning (LIPoC) against cerebral ischemia reperfusion in rats and the relationship between cycle number or remote postconditioning episode duration and the protective function of LIPoC.  Materials and methods: Transient focal ischemia/reperfusion was induced by 90 min of middle cerebral artery occlusion (MCAO) with nylon monofilament and 22 h of reperfusion in male Sprague-Dawley rats. The animals underwent LIPoC consisting of 1, 2, or 3 cycles, with each occlusion or release lasting for 5 min (5/5), 10 min (10/10), or 15 min (15/15) of bilateral femoral artery occlusion/release. Neurological score, infarct volume, water content, blood-brain barrier permeability (BBB), neuronal analysis, immunohistochemical detection, and mitochondrial permeability transition pore opening were measured.  Results: Infarct volume was significantly reduced in the groups with 2 cycles of 15/15, 3 cycles of 10/10, and 2 cycles of 10/10. These 3 groups also had attenuated brain edema, BBB disruption, and apoptosis compared to both the MCAO and sham groups.  Conclusion: This study affirmed the protective effect of LIPoC on cerebral reperfusion injury. Furthermore, it indicated that the accumulative time of limb occlusion/reperfusion might be crucial in remote postconditioning and that LIPoC exerted its maximum protective effect if the accumulative time of limb occlusion/reperfusion lasted from 40 to 60 min.

Makale detayları

Limb remote ischemic postconditioning is effective but also time-course-limited in protecting the brain from I/R injury

Yazarlar:

Ce XU | Chenju YI | Haidong GUO | Qiangqiang LIU | Li WANG | Hongxia LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.918-929

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754056

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Stroke

ischemia/reperfusion injury

middle cerebral artery occlusion

remote ischemic postconditioning

limb

rat

Sayı

Makale detayları

Limb remote ischemic postconditioning is effective but also time-course-limited in protecting the brain from I/R injury

Yazarlar: Ce XU | Chenju YI | Haidong GUO | Qiangqiang LIU | Li WANG | Hongxia LI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.918-929

Belge Tipi: Makale

Yayın Tarihi: 4/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000754056

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Stroke

ischemia/reperfusion injury

middle cerebral artery occlusion

remote ischemic postconditioning

limb

rat

Sayı

Yazarlar:

Ce XU | Chenju YI | Haidong GUO | Qiangqiang LIU | Li WANG | Hongxia LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue 5, pp.918-929

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000754056

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144