NEUROPROTECTIVE EFFECT OF CHLORZOXAZONE AGAINST GLUTAMATE TOXICITY IN RAT PRIMARY CORTEX NEURON CULTURE

Yeşim Yeni; Betul Cicek; Ahmet Hacımüftüoğlu

Makale detayları

NEUROPROTECTIVE EFFECT OF CHLORZOXAZONE AGAINST GLUTAMATE TOXICITY IN RAT PRIMARY CORTEX NEURON CULTURE

Yazarlar:

Yeşim Yeni | Betul Cicek | Ahmet Hacımüftüoğlu

Yayıncı:

Erciyes University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Sağlık Bilimleri Dergisi Vol.33 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335070

pISSN:

1018-3655

Anahtar kelimeler:

Özet:

 Glutamate Glut toxicity is one of the main causes of neurological diseases. Chlorzoxazone CZ is a muscle relaxant used to decrease pain and inflammation associated with acute and chronic twists and bruises. Here, we objected to research the neuroprotective effect of CZ applied to reverse Glut-induced neurodegeneration in the neonatal cerebral cortex through anti-inflammatory and antioxidant mechanisms. Neonatal cortical neurons were exposed to Glut and different doses of CZ 10, 20, and 40 µM were applied to assess the effect of CZ on Glut toxicity. We then examined changes in cell viability, inflammation, and oxidative stress. Our cell viability analysis showed that CZ protected cells from Glut-induced neuronal damage. In addition, the neuroprotective properties of CZ were evaluated by examining oxidative and antioxidant parameters such as MDA, MPO, CAT, GSH, GPx, and SOD. In line with the data obtained, it was observed that the cell viability rate decreased to 60 in the Glut group. However, with CZ application, the most significant increase in cell viability was seen at the 40 μM dose 86, while the least increase was seen at 10 μM CZ 77. It also proved that CZ increased the activity of antioxidant parameters while reducing oxidative parameters and inflammation. Therefore, the present findings collectively demonstrated that CZ potently inhibits Glut-induced injury in neonatal cortical neurons. The present work is the initial to show the protective effect of CZ in neonatal cortical neurons exposed to Glut excitotoxicity and suggesting that CZ may be used as a therapeutic agent.

 Glutamate Glut toxicity is one of the main causes of neurological diseases. Chlorzoxazone CZ is a muscle relaxant used to decrease pain and inflammation associated with acute and chronic twists and bruises. Here, we objected to research the neuroprotective effect of CZ applied to reverse Glut-induced neurodegeneration in the neonatal cerebral cortex through anti-inflammatory and antioxidant mechanisms. Neonatal cortical neurons were exposed to Glut and different doses of CZ 10, 20, and 40 µM were applied to assess the effect of CZ on Glut toxicity. We then examined changes in cell viability, inflammation, and oxidative stress. Our cell viability analysis showed that CZ protected cells from Glut-induced neuronal damage. In addition, the neuroprotective properties of CZ were evaluated by examining oxidative and antioxidant parameters such as MDA, MPO, CAT, GSH, GPx, and SOD. In line with the data obtained, it was observed that the cell viability rate decreased to 60 in the Glut group. However, with CZ application, the most significant increase in cell viability was seen at the 40 μM dose 86, while the least increase was seen at 10 μM CZ 77. It also proved that CZ increased the activity of antioxidant parameters while reducing oxidative parameters and inflammation. Therefore, the present findings collectively demonstrated that CZ potently inhibits Glut-induced injury in neonatal cortical neurons. The present work is the initial to show the protective effect of CZ in neonatal cortical neurons exposed to Glut excitotoxicity and suggesting that CZ may be used as a therapeutic agent.

 Glutamate Glut toxicity is one of the main causes of neurological diseases. Chlorzoxazone CZ is a muscle relaxant used to decrease pain and inflammation associated with acute and chronic twists and bruises. Here, we objected to research the neuroprotective effect of CZ applied to reverse Glut-induced neurodegeneration in the neonatal cerebral cortex through anti-inflammatory and antioxidant mechanisms. Neonatal cortical neurons were exposed to Glut and different doses of CZ 10, 20, and 40 µM were applied to assess the effect of CZ on Glut toxicity. We then examined changes in cell viability, inflammation, and oxidative stress. Our cell viability analysis showed that CZ protected cells from Glut-induced neuronal damage. In addition, the neuroprotective properties of CZ were evaluated by examining oxidative and antioxidant parameters such as MDA, MPO, CAT, GSH, GPx, and SOD. In line with the data obtained, it was observed that the cell viability rate decreased to 60 in the Glut group. However, with CZ application, the most significant increase in cell viability was seen at the 40 μM dose 86, while the least increase was seen at 10 μM CZ 77. It also proved that CZ increased the activity of antioxidant parameters while reducing oxidative parameters and inflammation. Therefore, the present findings collectively demonstrated that CZ potently inhibits Glut-induced injury in neonatal cortical neurons. The present work is the initial to show the protective effect of CZ in neonatal cortical neurons exposed to Glut excitotoxicity and suggesting that CZ may be used as a therapeutic agent.

 Glutamat Glut toksisitesi, nörolojik hastalıklara zemin hazırlayan ana sebeplerden biridir. Klorzoksazon CZ, akut ve kronik morluklar ve burkulmalarla ilişkili ağrı ve iltihabı azaltmak için kullanılan kas gevşeticidir. Burada, yenidoğan serebral kortekste Glutun neden olduğu nörodejenerasyonu tersine çevirmek için uygulanan CZnin nöroprotektif etkisini anti-inflamatuar ve antioksidan mekanizmalar yoluyla araştırmayı amaçladık. Yenidoğan kortikal nöronları Gluta maruz bırakıldı ve CZnin Glut toksisitesi üzerindeki etkisini değerlendirmek için CZ çeşitli dozlarda 10, 20 ve 40 µM uygulandı. Ardından hücre canlılığı, oksidatif stres ve inflamasyondaki değişiklikleri inceledik. Hücre canlılık analizimiz, CZnin Glut kaynaklı nöranal hasardan hücreleri koruduğunu göstermiştir. Ayrıca CZnin nöroprotektif özelliği MDA, MPO, CAT, GSH, GPx ve SOD gibi oksidatif ve antioksidan parametrelerin incelenmesi ile değerlendirildi. Elde edilen veriler doğrultusunda,hücre canlılık oranı Glut grubunda  60a kadar düştüğü gözlendi. Ancak CZ uygulaması ile birlikte hücre canlılığında en anlamlı artış 40 μM dozunda 86 görülürken, en az artış 10 μM CZ 77 görüldü. Ayrıca CZnin oksidatif parametreleri ve inflamasyonu azaltırken, antioksidan parametrelerin aktivitesini arttırdığını kanıtladı. Bu nedenle mevcut bulgular toplu olarak CZnin Glut kaynaklı hasarı yenidoğan kortikal nöronlarında güçlü bir şekilde önlediğini göstermiştir. Mevcut çalışma, Glut eksitotoksisitesine maruz kalan yenidoğan korteks nöronlarında CZnin koruyucu etkisini gösteren ilk çalışmadır ve CZnin terapötik bir ajan olarak kullanılabileceğini göstermektedir.

Makale detayları

NEUROPROTECTIVE EFFECT OF CHLORZOXAZONE AGAINST GLUTAMATE TOXICITY IN RAT PRIMARY CORTEX NEURON CULTURE

Yazarlar:

Yeşim Yeni | Betul Cicek | Ahmet Hacımüftüoğlu

Yayıncı:

Erciyes University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Sağlık Bilimleri Dergisi Vol.33 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335070

pISSN:

1018-3655

Chlorzoxazone

cortex

glutamate

neuronKlorzoksazon

korteks

glutamat

nöron

Sayı

Makale detayları

Yazarlar: Yeşim Yeni | Betul Cicek | Ahmet Hacımüftüoğlu

Yayıncı: Erciyes University

Ana Başlıklar: Tıbbi Farmakoloji

Dergi: Sağlık Bilimleri Dergisi Vol.33 Issue 3, pp.289-295

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001335070

pISSN: 1018-3655

Sayı

Yazarlar:

Yeşim Yeni | Betul Cicek | Ahmet Hacımüftüoğlu

Yayıncı:

Erciyes University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Sağlık Bilimleri Dergisi Vol.33 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001335070

pISSN:

1018-3655