In vivo assessment of DNA damage in Cyprinus carpio after exposure to potassium dichromate using RAPD

Pavan KUMAR; Ravindra KUMAR; Naresh Sahebrao NAGPURE; Prakash NAUTIYAL; Basdeo KUSHWAHA; Christopher Didigwu NWANI; Wazir Singh LAKRA

Makale detayları

In vivo assessment of DNA damage in Cyprinus carpio after exposure to potassium dichromate using RAPD

Yazarlar:

Pavan KUMAR | Ravindra KUMAR | Naresh Sahebrao NAGPURE | Prakash NAUTIYAL | Basdeo KUSHWAHA | Christopher Didigwu NWANI | Wazir Singh LAKRA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.121-127

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765610

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed differences in RAPD profiles with respect to the disappearance of several bands and the appearance of new ones. There was a significant (P < 0.05) difference between 72 and 96 h after exposure with respect to genomic template stability. The variation in RAPD profiles indicated both time- and concentration-dependent relationships. The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.

 

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed differences in RAPD profiles with respect to the disappearance of several bands and the appearance of new ones. There was a significant (P < 0.05) difference between 72 and 96 h after exposure with respect to genomic template stability. The variation in RAPD profiles indicated both time- and concentration-dependent relationships. The results suggested significant genomic template instability as a measurement of DNA damage and further demonstrated the potential of RAPD as a powerful tool for detecting genotoxicity induced by potassium dichromate in aquatic environments.

Makale detayları

In vivo assessment of DNA damage in Cyprinus carpio after exposure to potassium dichromate using RAPD

Yazarlar: Pavan KUMAR | Ravindra KUMAR | Naresh Sahebrao NAGPURE | Prakash NAUTIYAL | Basdeo KUSHWAHA | Christopher Didigwu NWANI | Wazir Singh LAKRA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.121-127

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765610

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Cyprinus carpio

DNA damage

potassium dichromate

RAPD

Sayı

Yazarlar:

Pavan KUMAR | Ravindra KUMAR | Naresh Sahebrao NAGPURE | Prakash NAUTIYAL | Basdeo KUSHWAHA | Christopher Didigwu NWANI | Wazir Singh LAKRA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.121-127

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765610

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128