Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Azra Zahrah Nadhirah Ikhwani; Idris Idris; Rizki Rabeca Elfirta; Pamungkas Rizki Ferdian

Makale detayları

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Yazarlar:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001309563

pISSN:

2149-0120

Anahtar kelimeler:

Özet:

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

Makale detayları

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Yazarlar:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001309563

pISSN:

2149-0120

Bacillus flexus InaCC-B486

lipase enzyme

production optimization.

Sayı

Makale detayları

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Yazarlar: Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Biochemistry and Cell Biology (Other)

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001309563

pISSN: 2149-0120

Bacillus flexus InaCC-B486

lipase enzyme

production optimization.

Sayı

Yazarlar:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001309563

pISSN:

2149-0120