GRAPHENE OXIDE HAS A NEUROPROTECTIVE EFFECT AGAINST GLUTAMATE-INDUCED EXCITOXICITY ON B35 NEUROBLASTOMA CELL LINE

Buse Kayhan; Şeyma Taşdemir; Pelin Çoruk İLHAN; Cansu GÖRGÜN; Aylin Şendemir ÜRKMEZ; Gülgün ŞENGÜL

Makale detayları

GRAPHENE OXIDE HAS A NEUROPROTECTIVE EFFECT AGAINST GLUTAMATE-INDUCED EXCITOXICITY ON B35 NEUROBLASTOMA CELL LINE

Yazarlar:

Buse Kayhan | Şeyma Taşdemir | Pelin Çoruk İLHAN | Cansu GÖRGÜN | Aylin Şendemir ÜRKMEZ | Gülgün ŞENGÜL

Yayıncı:

Society of Anatomy and Clinical Anatomy

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Anatomy 2015 Vol.9 Issue 3, pp.156-167

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000668441

Sıklık:

Üç Yıllık

eISSN:

1308-8459

Özet:

 


Objectives: Graphene is a quasi–two-dimensional material with unique electrical and chemical properties. In terms of biomedical applications of graphene, nervous system would be an ideal breakthrough model because neural cells are electroactive. Extreme glutamate concentrations cause excitotoxicity. In this study, we aimed to investigate if graphene can increasethe resistance to glutamate stress in B35 rat neuroblastoma cells as a cultured cell model for central nervous system neurons.Methods: B35 neuroblastoma cells were grown in DMEM-F12 growth medium containing 10% fetal bovine serum. Graphene oxide (GO) powder was coated onto glass slides with chitosan as a thin film. B35 cells were cultured on GO films. Cells cultivated on glass slides were used as controls. After 24 h of cell culture, L-glutamine induced excitotoxicity was imposed on B35 cells. After 24 h of glutamate-induced stress, cell morphology was examined by scanning electron microscopy. Cell viability was measured with MTT assay.Results: The effects of glutamate stress on cell viability were visible as early as 1 h. The cell viability on GO films was higher than that on glass slides, and cells recovered from stress within 6 h on GO surfaces. After 24 h, viability on glass surfaces was 54% lower than that on GO surfaces; these findings were supported with cell morphology observations..Conclusion: The results of this study showed that GO has a protective role in reducing glutamate-induced excitotoxicity in B35 cell culture, indicating a potential use of GO for treatment of excitotoxicity induced neurodegenerative diseases.

.

Makale detayları

GRAPHENE OXIDE HAS A NEUROPROTECTIVE EFFECT AGAINST GLUTAMATE-INDUCED EXCITOXICITY ON B35 NEUROBLASTOMA CELL LINE

Yazarlar: Buse Kayhan | Şeyma Taşdemir | Pelin Çoruk İLHAN | Cansu GÖRGÜN | Aylin Şendemir ÜRKMEZ | Gülgün ŞENGÜL

Yayıncı: Society of Anatomy and Clinical Anatomy

Ana Başlıklar: Sağlık teşvikleri

Dergi: Anatomy 2015 Vol.9 Issue 3, pp.156-167

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: İngilizce

Document ID (Org No.): 2000668441

Sıklık: Üç Yıllık

eISSN: 1308-8459

Sayı

Yazarlar:

Buse Kayhan | Şeyma Taşdemir | Pelin Çoruk İLHAN | Cansu GÖRGÜN | Aylin Şendemir ÜRKMEZ | Gülgün ŞENGÜL

Yayıncı:

Society of Anatomy and Clinical Anatomy

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Anatomy 2015 Vol.9 Issue 3, pp.156-167

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000668441

Sıklık:

Üç Yıllık

eISSN:

1308-8459