Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability

Ümit Erdoğan

Makale detayları

Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability

Yazarlar:

Ümit Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.231-239

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289444

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH and CUPRAC- hydroxyl radical scavenging HRS methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components 189 mg GAE g extract. According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43  0.07 mmol TEg-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TEg-propolis extract. DPPH scavenging activity of propolis extract was calculated to be 0.71 0.002 mmol TE  g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations 5-10 μgmL was determined as 68.1 and 77.64 , respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.

 In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH and CUPRAC- hydroxyl radical scavenging HRS methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components 189 mg GAE g extract. According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43  0.07 mmol TEg-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TEg-propolis extract. DPPH scavenging activity of propolis extract was calculated to be 0.71 0.002 mmol TE  g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations 5-10 μgmL was determined as 68.1 and 77.64 , respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.

 In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH and CUPRAC- hydroxyl radical scavenging HRS methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components 189 mg GAE g extract. According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43  0.07 mmol TEg-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TEg-propolis extract. DPPH scavenging activity of propolis extract was calculated to be 0.71 0.002 mmol TE  g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations 5-10 μgmL was determined as 68.1 and 77.64 , respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.

 In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH and CUPRAC- hydroxyl radical scavenging HRS methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components 189 mg GAE g extract. According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43  0.07 mmol TEg-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TEg-propolis extract. DPPH scavenging activity of propolis extract was calculated to be 0.71 0.002 mmol TE  g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations 5-10 μgmL was determined as 68.1 and 77.64 , respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.

Makale detayları

Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability

Yazarlar:

Ümit Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.231-239

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289444

pISSN:

2148-6905

Propolis

FTIR

CUPRAC assay

DPPH

FRAPPropolis

FTIR

CUPRAC Assay

DPPH

FRAP

Sayı

Makale detayları

Yazarlar: Ümit Erdoğan

Yayıncı: İzzet KARA

Ana Başlıklar: Structural Biology

Dergi: International Journal of Secondary Metabolite Vol.10 Issue 2, pp.231-239

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001289444

pISSN: 2148-6905

Sayı

Yazarlar:

Ümit Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.231-239

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289444

pISSN:

2148-6905