Antibacterial and Anti-Biofilm Effect of Silver Nanoparticles Synthesized from Origanum majorana and Echinacea purpurea (L.) Moench Plants via Green Synthesis

Ayşe Karacalı Tunç; Büşra Merve Sarıtaş; Ramazan Erenler

Makale detayları

Antibacterial and Anti-Biofilm Effect of Silver Nanoparticles Synthesized from Origanum majorana and Echinacea purpurea (L.) Moench Plants via Green Synthesis

Yazarlar:

Ayşe Karacalı Tunç | Büşra Merve Sarıtaş | Ramazan Erenler

Yayıncı:

Kocaeli University

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 2, pp.48-52

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326660

pISSN:

2149-8571

Anahtar kelimeler:

Özet:

 Objective: Nanotechnology has gained importance in the fight against epidemics and antibiotic resistance. Nanotechnology is a potential way to prevent the increase of multidrug-resistant bacterial species. The aim of this study was to determine the antimicrobial and anti-biofilm activities of Origanum majorana and Echinacea purpurea silver nanoparticles AgNPs against Staphylococcus aureus S.aureus ATCC 25923, Escherichia coli E.coli 25922, Pseudomonas aureginosa P.aureginosa 27853, Klebsiella pneumoniae K. pneumoniae 700603. Methods: Minimum Inhibitory Concentration MIC values of silver nanoparticles were determined by microdilution method in 96-well ELISA plates. The anti-biofilm effect of silver nanoparticles was performed by crystal violet method in 96-well microplates. Results: The MIC value of four isolates was determined as 128 µgml for two nanoparticles. Only 256 µgml and 512 µgml were found for S. aureus. In vitro, anti-biofilm effect of AgNPs against biofilm forming bacteria was evaluated in a dose-dependent manner. S. aureus, AgNP synthesized from Origanum majorana plant extract 512 µgml reduced biofilm formation by 92 after 24 hours of incubation. As a result of 24 hours incubation of S. aureus with AgNP 512 µgml synthesized from Echinacea purpurea L. It was determined that biofilm formation decreased by 85. It was observed that both different nanoparticles significantly inhibited the biofilm mass. Conclusion: AgNPs showed antimicrobial and antibiofilm effects for standard strains. The use of AgNPs as antimicrobials is promising for the future.

 Objective: Nanotechnology has gained importance in the fight against epidemics and antibiotic resistance. Nanotechnology is a potential way to prevent the increase of multidrug-resistant bacterial species. The aim of this study was to determine the antimicrobial and anti-biofilm activities of Origanum majorana and Echinacea purpurea silver nanoparticles AgNPs against Staphylococcus aureus S.aureus ATCC 25923, Escherichia coli E.coli 25922, Pseudomonas aureginosa P.aureginosa 27853, Klebsiella pneumoniae K. pneumoniae 700603. Methods: Minimum Inhibitory Concentration MIC values of silver nanoparticles were determined by microdilution method in 96-well ELISA plates. The anti-biofilm effect of silver nanoparticles was performed by crystal violet method in 96-well microplates. Results: The MIC value of four isolates was determined as 128 µgml for two nanoparticles. Only 256 µgml and 512 µgml were found for S. aureus. In vitro, anti-biofilm effect of AgNPs against biofilm forming bacteria was evaluated in a dose-dependent manner. S. aureus, AgNP synthesized from Origanum majorana plant extract 512 µgml reduced biofilm formation by 92 after 24 hours of incubation. As a result of 24 hours incubation of S. aureus with AgNP 512 µgml synthesized from Echinacea purpurea L. It was determined that biofilm formation decreased by 85. It was observed that both different nanoparticles significantly inhibited the biofilm mass. Conclusion: AgNPs showed antimicrobial and antibiofilm effects for standard strains. The use of AgNPs as antimicrobials is promising for the future.

 Objective: Nanotechnology has gained importance in the fight against epidemics and antibiotic resistance. Nanotechnology is a potential way to prevent the increase of multidrug-resistant bacterial species. The aim of this study was to determine the antimicrobial and anti-biofilm activities of Origanum majorana and Echinacea purpurea silver nanoparticles AgNPs against Staphylococcus aureus S.aureus ATCC 25923, Escherichia coli E.coli 25922, Pseudomonas aureginosa P.aureginosa 27853, Klebsiella pneumoniae K. pneumoniae 700603. Methods: Minimum Inhibitory Concentration MIC values of silver nanoparticles were determined by microdilution method in 96-well ELISA plates. The anti-biofilm effect of silver nanoparticles was performed by crystal violet method in 96-well microplates. Results: The MIC value of four isolates was determined as 128 µgml for two nanoparticles. Only 256 µgml and 512 µgml were found for S. aureus. In vitro, anti-biofilm effect of AgNPs against biofilm forming bacteria was evaluated in a dose-dependent manner. S. aureus, AgNP synthesized from Origanum majorana plant extract 512 µgml reduced biofilm formation by 92 after 24 hours of incubation. As a result of 24 hours incubation of S. aureus with AgNP 512 µgml synthesized from Echinacea purpurea L. It was determined that biofilm formation decreased by 85. It was observed that both different nanoparticles significantly inhibited the biofilm mass. Conclusion: AgNPs showed antimicrobial and antibiofilm effects for standard strains. The use of AgNPs as antimicrobials is promising for the future.

 Objective: Nanotechnology has gained importance in the fight against epidemics and antibiotic resistance. Nanotechnology is a potential way to prevent the increase of multidrug-resistant bacterial species. The aim of this study was to determine the antimicrobial and anti-biofilm activities of Origanum majorana and Echinacea purpurea silver nanoparticles AgNPs against Staphylococcus aureus S.aureus ATCC 25923, Escherichia coli E.coli 25922, Pseudomonas aureginosa P.aureginosa 27853, Klebsiella pneumoniae K. pneumoniae 700603. Methods: Minimum Inhibitory Concentration MIC values of silver nanoparticles were determined by microdilution method in 96-well ELISA plates. The anti-biofilm effect of silver nanoparticles was performed by crystal violet method in 96-well microplates. Results: The MIC value of four isolates was determined as 128 µgml for two nanoparticles. Only 256 µgml and 512 µgml were found for S. aureus. In vitro, anti-biofilm effect of AgNPs against biofilm forming bacteria was evaluated in a dose-dependent manner. S. aureus, AgNP synthesized from Origanum majorana plant extract 512 µgml reduced biofilm formation by 92 after 24 hours of incubation. As a result of 24 hours incubation of S. aureus with AgNP 512 µgml synthesized from Echinacea purpurea L. It was determined that biofilm formation decreased by 85. It was observed that both different nanoparticles significantly inhibited the biofilm mass. Conclusion: AgNPs showed antimicrobial and antibiofilm effects for standard strains. The use of AgNPs as antimicrobials is promising for the future.

Makale detayları

Antibacterial and Anti-Biofilm Effect of Silver Nanoparticles Synthesized from Origanum majorana and Echinacea purpurea (L.) Moench Plants via Green Synthesis

Yazarlar: Ayşe Karacalı Tunç | Büşra Merve Sarıtaş | Ramazan Erenler

Yayıncı: Kocaeli University

Ana Başlıklar: Mikrobiyoloji (Diğer)

Dergi: Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 2, pp.48-52

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326660

pISSN: 2149-8571

Silver Nanoparticles

antibiotic resistance

Origanum majorana

Echinacea purpurea

Sayı

Yazarlar:

Ayşe Karacalı Tunç | Büşra Merve Sarıtaş | Ramazan Erenler

Yayıncı:

Kocaeli University

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 2, pp.48-52

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326660

pISSN:

2149-8571