Proximity mapping of the microtubule plus-end tracking protein SLAIN2 using the BioID approach

Elif Nur Fırat KARALAR

doi:https://doi.org/10.3906/biy-2002-12

Makale detayları

Proximity mapping of the microtubule plus-end tracking protein SLAIN2 using the BioID approach

Yazarlar:

Elif Nur Fırat KARALAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2020 Vol.44 Issue 2, pp.61-72

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000811011

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-2002-12

Özet:

 

The centrosome is the main microtubule-organizing center of animal cells, which plays key roles in critical cellular processes  ranging from cell division to cellular signaling. Accordingly, defects in the structure and function of centrosomes cause various human  diseases such as cancer and primary microcephaly. To elucidate the molecular defects underlying these diseases, the biogenesis and  functions of the centrosomes have to be fully understood. An essential step towards addressing these questions is the identification  and functional dissection of the full repertoire of centrosome proteins. Here, we used high-resolution imaging and showed that the  microtubule plus-end tracking protein SLAIN2 localizes to the pericentriolar material at the proximal end of centrioles. To gain insight  into its cellular functions and mechanisms, we applied in vivo proximity-dependent biotin identification to SLAIN2 and generated its  proximity interaction map. Gene ontology analysis of the SLAIN2 interactome revealed extensive interactions with centriole duplication,  ciliogenesis, and microtubule-associated proteins, including previously characterized and uncharacterized interactions. Collectively,  our results define SLAIN2 as a component of pericentriolar material and provide an important resource for future studies aimed at  elucidating SLAIN2 functions at the centrosome.

Makale detayları

Proximity mapping of the microtubule plus-end tracking protein SLAIN2 using the BioID approach

Yazarlar: Elif Nur Fırat KARALAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2020 Vol.44 Issue 2, pp.61-72

Belge Tipi: Makale

Yayın Tarihi: 2/2020

Belge Dili: Türkçe

Document ID (Org No.): 2000811011

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-2002-12

Sayı

Yazarlar:

Elif Nur Fırat KARALAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2020 Vol.44 Issue 2, pp.61-72

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000811011

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-2002-12