Investigation of Cytokines, Biochemical Parameters and Oxidative Stress Levels in Serum of Patients with Acute Pancreatitis

Nihayet Bayraktar; Oruç Aslan; Ahmet Uyanıkoğlu; Ali Öztürk; Mehmet Bayraktar; Hamza Erdoğdu

Makale detayları

Investigation of Cytokines, Biochemical Parameters and Oxidative Stress Levels in Serum of Patients with Acute Pancreatitis

Yazarlar:

Nihayet Bayraktar | Oruç Aslan | Ahmet Uyanıkoğlu | Ali Öztürk | Mehmet Bayraktar | Hamza Erdoğdu

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Genel Tıp Dergisi Vol.34 Issue 2, pp.158-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316095

pISSN:

2602-3741

Anahtar kelimeler:

Özet:

 Objective: This study aimed to find new serum biochemistry parameters, especially for the early identification of severe AP. In the study serum cytokine levels TNF-A, IL-1, IL-6, IL-10, IL-21, biochemical parameters Aquaporin-1, Hepcidine, Iron, Zinc, Copper, Nitric Oxide, C-Reactive protein, and oxidative stress parameters analysis were performed. Method: A total of 46 patients with AP and 46 healthy controls were included in this study. Serum cytokine levels, biochemical, and oxidative stress parameters by the ELISA method, and CRP by the immunoturbidimetric method were measured. Results: When patients with AP and control groups were compared, all studied parameters were found to be statistically significant p0.05. However, IL-1 was found to be insignificant p0.05. Therefore, the Mann-Whitney U test, which is a non-parametric test, was found suitable to determine whether there was a significant difference between the patient and control groups. Conclusions: Investigating the role of cytokines, oxidative stress and other biochemical parameters in the pathogenesis and course of the disease may contribute to a better understanding of the disease process and its therapeutic value. It showed a significant increase in oxidative stress parameters and biochemical parameters such as aquaporin-1, hepcidin, lipase, and amylase, which may help in the diagnosis of AP.

 Objective: This study aimed to find new serum biochemistry parameters, especially for the early identification of severe AP. In the study serum cytokine levels TNF-A, IL-1, IL-6, IL-10, IL-21, biochemical parameters Aquaporin-1, Hepcidine, Iron, Zinc, Copper, Nitric Oxide, C-Reactive protein, and oxidative stress parameters analysis were performed. Method: A total of 46 patients with AP and 46 healthy controls were included in this study. Serum cytokine levels, biochemical, and oxidative stress parameters by the ELISA method, and CRP by the immunoturbidimetric method were measured. Results: When patients with AP and control groups were compared, all studied parameters were found to be statistically significant p0.05. However, IL-1 was found to be insignificant p0.05. Therefore, the Mann-Whitney U test, which is a non-parametric test, was found suitable to determine whether there was a significant difference between the patient and control groups. Conclusions: Investigating the role of cytokines, oxidative stress and other biochemical parameters in the pathogenesis and course of the disease may contribute to a better understanding of the disease process and its therapeutic value. It showed a significant increase in oxidative stress parameters and biochemical parameters such as aquaporin-1, hepcidin, lipase, and amylase, which may help in the diagnosis of AP.

 Objective: This study aimed to find new serum biochemistry parameters, especially for the early identification of severe AP. In the study serum cytokine levels TNF-A, IL-1, IL-6, IL-10, IL-21, biochemical parameters Aquaporin-1, Hepcidine, Iron, Zinc, Copper, Nitric Oxide, C-Reactive protein, and oxidative stress parameters analysis were performed. Method: A total of 46 patients with AP and 46 healthy controls were included in this study. Serum cytokine levels, biochemical, and oxidative stress parameters by the ELISA method, and CRP by the immunoturbidimetric method were measured. Results: When patients with AP and control groups were compared, all studied parameters were found to be statistically significant p0.05. However, IL-1 was found to be insignificant p0.05. Therefore, the Mann-Whitney U test, which is a non-parametric test, was found suitable to determine whether there was a significant difference between the patient and control groups. Conclusions: Investigating the role of cytokines, oxidative stress and other biochemical parameters in the pathogenesis and course of the disease may contribute to a better understanding of the disease process and its therapeutic value. It showed a significant increase in oxidative stress parameters and biochemical parameters such as aquaporin-1, hepcidin, lipase, and amylase, which may help in the diagnosis of AP.

 Amaç: Bu çalışma, özellikle şiddetli APnin erken teşhisi için yeni serum biyokimya parametreleri bulmayı amaçladı. Çalışmada serum sitokin seviyeleri TNF-A, IL-1, IL-6, IL-10, IL-21, biyokimyasal parametreler Aquaporin-1, Hepsidin, Demir, Çinko, Bakır, Nitrik Oksit, C-Reaktif protein ve oksidatif stres parametreleri analizi yapıldı. Yöntem: Bu çalışmaya APli toplam 46 hasta ve 46 sağlıklı kontrol dahil edildi. ELISA yöntemi ile serum sitokin düzeyleri, biyokimyasal ve oksidatif stres parametreleri, immünotürbidimetrik yöntemle de CRP ölçüldü. Bulgular: APli hastalar ve kontrol grubu karşılaştırıldığında çalışılan tüm parametreler istatistiksel olarak anlamlı bulundu p0.05. Ancak IL-1in önemsiz olduğu bulundu p0.05. Bu nedenle hasta ve kontrol grupları arasında anlamlı fark olup olmadığını belirlemek için parametrik olmayan bir test olan Mann-Whitney U testi uygun bulunmuştur. Sonuç: Sitokinlerin, oksidatif stresin ve diğer biyokimyasal parametrelerin hastalığın patogenezindeki ve seyrindeki rolünün araştırılması, hastalık sürecinin ve tedavi edici değerinin daha iyi anlaşılmasına katkı sağlayabilir. AP tanısında yardımcı olabilecek aquaporin-1, hepsidin, lipaz ve amilaz gibi oksidatif stres parametrelerinde ve biyokimyasal parametrelerde önemli bir artış gösterdi.

Makale detayları

Investigation of Cytokines, Biochemical Parameters and Oxidative Stress Levels in Serum of Patients with Acute Pancreatitis

Yazarlar:

Nihayet Bayraktar | Oruç Aslan | Ahmet Uyanıkoğlu | Ali Öztürk | Mehmet Bayraktar | Hamza Erdoğdu

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Genel Tıp Dergisi Vol.34 Issue 2, pp.158-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316095

pISSN:

2602-3741

Acute pancreatitis

cytokine

oxidative stress

some other biochemical parametersAkut pankreatit

sitokin

oksidatif stres

diğer bazı biyokimyasal parametreler

Sayı

Makale detayları

Yazarlar: Nihayet Bayraktar | Oruç Aslan | Ahmet Uyanıkoğlu | Ali Öztürk | Mehmet Bayraktar | Hamza Erdoğdu

Yayıncı: Selcuk University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Genel Tıp Dergisi Vol.34 Issue 2, pp.158-165

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001316095

pISSN: 2602-3741

Sayı

Yazarlar:

Nihayet Bayraktar | Oruç Aslan | Ahmet Uyanıkoğlu | Ali Öztürk | Mehmet Bayraktar | Hamza Erdoğdu

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Genel Tıp Dergisi Vol.34 Issue 2, pp.158-165

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316095

pISSN:

2602-3741