Bacterial Biodiversity of the Kapova Karst Cave as a Source of Hydrolases Producers

William Kurdy; Galina Yakovleva; Olga Ilinskaya

Makale detayları

Bacterial Biodiversity of the Kapova Karst Cave as a Source of Hydrolases Producers

Yazarlar:

William Kurdy | Galina Yakovleva | Olga Ilinskaya

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.186-195

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310880

pISSN:

2602-2575

Anahtar kelimeler:

Özet:

 Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave ShulganTash Nature Reserve, Bashkortostan, and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate RNA, casein, starch. Functional profiles of the communitieswere predicted using the Global Mapper module on iVikodak. Taxonomic, structural, and compositional diversity were calculated using ShannonWiener and BrayCurtis indices. Results: Eighty-nine percent of 102 bacterial isolates were Proteobacteria, whereas other isolates were divided into three other phyla, Actinobacteria, Firmicutes, and Bacteroidetes that comprised 5, 4, and 2 of the isolates, respectively. Genus Pseudomonas was predominant with 42 isolates. Six isolates showed no extracellular enzymatic activity at all, 73 isolates expressed protease, 57 isolates expressed amylase, and 71 isolates had RNase activity. All three extracellular enzymes were expressed by 39isolates. Conclusion: The biodiversity of cultivated microbiota from Kapova Cave was characterized. Bacteria that produce large amounts of protease, RNase and amylase were identified as Stenotrophomonas rhizophila, Lysinibacillus fusiformis, and Pseudomonas stutzeri, respectively.

 Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave ShulganTash Nature Reserve, Bashkortostan, and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate RNA, casein, starch. Functional profiles of the communitieswere predicted using the Global Mapper module on iVikodak. Taxonomic, structural, and compositional diversity were calculated using ShannonWiener and BrayCurtis indices. Results: Eighty-nine percent of 102 bacterial isolates were Proteobacteria, whereas other isolates were divided into three other phyla, Actinobacteria, Firmicutes, and Bacteroidetes that comprised 5, 4, and 2 of the isolates, respectively. Genus Pseudomonas was predominant with 42 isolates. Six isolates showed no extracellular enzymatic activity at all, 73 isolates expressed protease, 57 isolates expressed amylase, and 71 isolates had RNase activity. All three extracellular enzymes were expressed by 39isolates. Conclusion: The biodiversity of cultivated microbiota from Kapova Cave was characterized. Bacteria that produce large amounts of protease, RNase and amylase were identified as Stenotrophomonas rhizophila, Lysinibacillus fusiformis, and Pseudomonas stutzeri, respectively.

 Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave ShulganTash Nature Reserve, Bashkortostan, and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate RNA, casein, starch. Functional profiles of the communitieswere predicted using the Global Mapper module on iVikodak. Taxonomic, structural, and compositional diversity were calculated using ShannonWiener and BrayCurtis indices. Results: Eighty-nine percent of 102 bacterial isolates were Proteobacteria, whereas other isolates were divided into three other phyla, Actinobacteria, Firmicutes, and Bacteroidetes that comprised 5, 4, and 2 of the isolates, respectively. Genus Pseudomonas was predominant with 42 isolates. Six isolates showed no extracellular enzymatic activity at all, 73 isolates expressed protease, 57 isolates expressed amylase, and 71 isolates had RNase activity. All three extracellular enzymes were expressed by 39isolates. Conclusion: The biodiversity of cultivated microbiota from Kapova Cave was characterized. Bacteria that produce large amounts of protease, RNase and amylase were identified as Stenotrophomonas rhizophila, Lysinibacillus fusiformis, and Pseudomonas stutzeri, respectively.

 Objective: Recent studies have revealed the biodiversity of both cultivated and uncultivated microbiomes in extreme environments. It has been shown that terrestrial subsurface ecosystems contain vast metabolic potential. Heterotrophic bacteria living in karst caves with an organic substrate deficit represent a special reserve for the isolation of metabolite producers. Here, we cultivated a bacterial community collected from biofilms in Kapova Cave ShulganTash Nature Reserve, Bashkortostan, and assessed its ability to synthesize secreted hydrolytic enzymes including RNases, proteases, and amylases. Materials and Methods: Isolated bacteria were identified by V3-V4 16S rRNA region sequencing. Enzymatic activities were assessed by measuring transparency zones around colonies grown on the appropriate substrate RNA, casein, starch. Functional profiles of the communitieswere predicted using the Global Mapper module on iVikodak. Taxonomic, structural, and compositional diversity were calculated using ShannonWiener and BrayCurtis indices. Results: Eighty-nine percent of 102 bacterial isolates were Proteobacteria, whereas other isolates were divided into three other phyla, Actinobacteria, Firmicutes, and Bacteroidetes that comprised 5, 4, and 2 of the isolates, respectively. Genus Pseudomonas was predominant with 42 isolates. Six isolates showed no extracellular enzymatic activity at all, 73 isolates expressed protease, 57 isolates expressed amylase, and 71 isolates had RNase activity. All three extracellular enzymes were expressed by 39isolates. Conclusion: The biodiversity of cultivated microbiota from Kapova Cave was characterized. Bacteria that produce large amounts of protease, RNase and amylase were identified as Stenotrophomonas rhizophila, Lysinibacillus fusiformis, and Pseudomonas stutzeri, respectively.

Makale detayları

Bacterial Biodiversity of the Kapova Karst Cave as a Source of Hydrolases Producers

Yazarlar:

William Kurdy | Galina Yakovleva | Olga Ilinskaya

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.186-195

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310880

pISSN:

2602-2575

Karst cave

biodiversity

cultivated bacteria

RNase

protease

amylase

Sayı

Makale detayları

Bacterial Biodiversity of the Kapova Karst Cave as a Source of Hydrolases Producers

Yazarlar: William Kurdy | Galina Yakovleva | Olga Ilinskaya

Yayıncı: Istanbul University

Ana Başlıklar: Structural Biology

Dergi: European Journal of Biology Vol.82 Issue 2, pp.186-195

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001310880

pISSN: 2602-2575

Karst cave

biodiversity

cultivated bacteria

RNase

protease

amylase

Sayı

Yazarlar:

William Kurdy | Galina Yakovleva | Olga Ilinskaya

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.186-195

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310880

pISSN:

2602-2575