Guaiakol Peroksidazın Soğan Köklerinden Afinite Kromatografisi ile Saflaştırılması

Aykut ÖZTEKİN

Makale detayları

Guaiakol Peroksidazın Soğan Köklerinden Afinite Kromatografisi ile Saflaştırılması

Yazarlar:

Aykut ÖZTEKİN

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2020 Vol.10 Issue 2, pp.1163-1170

Belge Tipi:

Makale

Yayın Tarihi:

5/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000464168

Sıklık:

Üç Aylık

eISSN:

2536-4618

Özet:

 


Peroxidases are enzymes that catalyze the oxidation of various organic substrates in the presence of hydrogen peroxide and contain heme group in their structure. In particular, plant peroxidases are frequently used in industry, clinical diagnosis, biosensor construction and organic synthesis reactions. Due to their commercial value, the identification and purification of these enzymes in different sources is of great importance. In this study, peroxidase enzyme was purified for the first time by using aminobenzohydrazide-based affinity chromatography technique from onion roots 750-fold with a yield of 37.7. SDS-PAGE was performed to determine the molecular weight of the purified enzyme and a single band was observed at 51.2 kDa. In addition, KM values of the enzyme for guaiacol, ABTS and pyrogallol substrates were calculated as 3.44, 0.46 and 21.27 mM, respectively.

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Makale detayları

Guaiakol Peroksidazın Soğan Köklerinden Afinite Kromatografisi ile Saflaştırılması

Yazarlar: Aykut ÖZTEKİN

Yayıncı: Igdir University

Ana Başlıklar: Kimya mühendisliği

Dergi: Journal of the Institute of Science and Technology 2020 Vol.10 Issue 2, pp.1163-1170

Belge Tipi: Makale

Yayın Tarihi: 5/2020

Belge Dili: Türkçe

Document ID (Org No.): 2000464168

Sıklık: Üç Aylık

eISSN: 2536-4618

Sayı

Yazarlar:

Aykut ÖZTEKİN

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2020 Vol.10 Issue 2, pp.1163-1170

Belge Tipi:

Makale

Yayın Tarihi:

5/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000464168

Sıklık:

Üç Aylık

eISSN:

2536-4618