Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Murat AKKURT; Atilla ÇAKIR; Mina SHİDFAR; Filiz MUTAF; Gökhan SÖYLEMEZOĞLU

Makale detayları

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Yazarlar:

Murat AKKURT | Atilla ÇAKIR | Mina SHİDFAR | Filiz MUTAF | Gökhan SÖYLEMEZOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.101-105

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812528

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

 

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

Makale detayları

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Yazarlar:

Murat AKKURT | Atilla ÇAKIR | Mina SHİDFAR | Filiz MUTAF | Gökhan SÖYLEMEZOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.101-105

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812528

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Grapevine

marker-assisted selection

seedlessness

Sayı

Makale detayları

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Yazarlar: Murat AKKURT | Atilla ÇAKIR | Mina SHİDFAR | Filiz MUTAF | Gökhan SÖYLEMEZOĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.101-105

Belge Tipi: Makale

Yayın Tarihi: 12/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000812528

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Grapevine

marker-assisted selection

seedlessness

Sayı

Yazarlar:

Murat AKKURT | Atilla ÇAKIR | Mina SHİDFAR | Filiz MUTAF | Gökhan SÖYLEMEZOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 1, pp.101-105

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000812528

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152