In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides `Cuba' - a multipurpose ornamental aquatic plant

Surendra BARPETE; Sancar Fatih ÖZCAN; Muhammad AASIM; Sebahattin ÖZCAN

Makale detayları

In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides `Cuba' - a multipurpose ornamental aquatic plant

Yazarlar:

Surendra BARPETE | Sancar Fatih ÖZCAN | Muhammad AASIM | Sebahattin ÖZCAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 3, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811943

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Hemianthus callitrichoides 'Cuba' is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-(γγ\gamma,γγ\gamma-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA3). Plant growth regulators significantly (P ≤ 0.001) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter (3.06 cm), area (6.12 cm), and clump weight (2.85 g) of apical shoots were achieved on MS medium containing 0.50 mg L-1 BAP. Higher concentrations of sucrose (9%) showed visible phenotypic growth inhibition and abnormalities were observed in vitro. The regenerated shoots rooted efficiently on MS medium without growth regulators. The growth pattern of multiple shoots indicated their origin from an enlarged shoot base via the proliferation of apical shoots. All in vitro regenerated plantlets acclimatized in aquariums successfully. The simplicity of the protocol and the direct production of multiple shoots make it a potential system that is highly amenable to true-to-type plant regeneration with the ability to maintain genetic stability.

 

Hemianthus callitrichoides 'Cuba' is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-(γγ\gamma,γγ\gamma-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA3). Plant growth regulators significantly (P ≤ 0.001) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter (3.06 cm), area (6.12 cm), and clump weight (2.85 g) of apical shoots were achieved on MS medium containing 0.50 mg L-1 BAP. Higher concentrations of sucrose (9%) showed visible phenotypic growth inhibition and abnormalities were observed in vitro. The regenerated shoots rooted efficiently on MS medium without growth regulators. The growth pattern of multiple shoots indicated their origin from an enlarged shoot base via the proliferation of apical shoots. All in vitro regenerated plantlets acclimatized in aquariums successfully. The simplicity of the protocol and the direct production of multiple shoots make it a potential system that is highly amenable to true-to-type plant regeneration with the ability to maintain genetic stability.

Makale detayları

In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides `Cuba' - a multipurpose ornamental aquatic plant

Yazarlar:

Surendra BARPETE | Sancar Fatih ÖZCAN | Muhammad AASIM | Sebahattin ÖZCAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 3, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811943

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Aquatic plant

apical shoot

Hemianthus callitrichoides

in vitro

regeneration

proliferation

plant growth regulators

Sayı

Makale detayları

Yazarlar: Surendra BARPETE | Sancar Fatih ÖZCAN | Muhammad AASIM | Sebahattin ÖZCAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 3, pp.493-500

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811943

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Surendra BARPETE | Sancar Fatih ÖZCAN | Muhammad AASIM | Sebahattin ÖZCAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 3, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811943

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152