A small intron of Drosophila gamma carboxylase, not predictable in silico for mammalian splicing machinery, is spliced improperly in human cells

SOLMAZ MONIRI JAVADHESARI; ALIREZA ZOMORODIPOUR; SPIDEH VALIMEHR

Makale detayları

A small intron of Drosophila gamma carboxylase, not predictable in silico for mammalian splicing machinery, is spliced improperly in human cells

Yazarlar:

SOLMAZ MONIRI JAVADHESARI | ALIREZA ZOMORODIPOUR | SPIDEH VALIMEHR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.674-681

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811836

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The splicing of two small introns of 58 and 67 nucleotides (nts) of the Drosophila gamma carboxylase (DγC) in cultured human cells was examined. The two introns are below the minimum size, which was proposed as a requirement for an intron to be spliced in human cells. None of the well-known software examined was able to provide a proper prediction for the DγC intron 1 in a mammalian host. Our in vivo experiments recognized only donor splice sites (not the acceptor site) of the DγC intron 1 by the mammalian cell, which, together with a new splicing acceptor site, generated an intron of 159 nts that was not predictable in silico. The experimental results showed that, for an intron to be spliced in mammalian splicing machinery, a minimum intron-length and acceptable branch point (BP) are more important than a pyrimidine-rich stretch in the 3'-region and wild-type splice sites. The in vivo approach, such as the one reported in this work, seems reliable for inspecting the interchangeability of introns among species.

 

The splicing of two small introns of 58 and 67 nucleotides (nts) of the Drosophila gamma carboxylase (DγC) in cultured human cells was examined. The two introns are below the minimum size, which was proposed as a requirement for an intron to be spliced in human cells. None of the well-known software examined was able to provide a proper prediction for the DγC intron 1 in a mammalian host. Our in vivo experiments recognized only donor splice sites (not the acceptor site) of the DγC intron 1 by the mammalian cell, which, together with a new splicing acceptor site, generated an intron of 159 nts that was not predictable in silico. The experimental results showed that, for an intron to be spliced in mammalian splicing machinery, a minimum intron-length and acceptable branch point (BP) are more important than a pyrimidine-rich stretch in the 3'-region and wild-type splice sites. The in vivo approach, such as the one reported in this work, seems reliable for inspecting the interchangeability of introns among species.

Makale detayları

A small intron of Drosophila gamma carboxylase, not predictable in silico for mammalian splicing machinery, is spliced improperly in human cells

Yazarlar:

SOLMAZ MONIRI JAVADHESARI | ALIREZA ZOMORODIPOUR | SPIDEH VALIMEHR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.674-681

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811836

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Gamma carboxylase

intron splicing

small intron

Drosophila melanogaster

Sayı

Makale detayları

A small intron of Drosophila gamma carboxylase, not predictable in silico for mammalian splicing machinery, is spliced improperly in human cells

Yazarlar: SOLMAZ MONIRI JAVADHESARI | ALIREZA ZOMORODIPOUR | SPIDEH VALIMEHR

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.674-681

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000811836

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Gamma carboxylase

intron splicing

small intron

Drosophila melanogaster

Sayı

Yazarlar:

SOLMAZ MONIRI JAVADHESARI | ALIREZA ZOMORODIPOUR | SPIDEH VALIMEHR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.674-681

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811836

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152