Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model

Yaşam ÇİRÇİRCİ; R. Nalan TİFTİK; İsmail ÜN

doi:https://doi.org/10.18621/eurj.900847

Makale detayları

Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model

Yazarlar:

Yaşam ÇİRÇİRCİ | R. Nalan TİFTİK | İsmail ÜN

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Hastalıklar

Dergi:

The European Research Journal 2021 Vol.7 Issue 3, pp.218-224

Belge Tipi:

Makale

Yayın Tarihi:

5/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000661928

Sıklık:

İki Aylık

eISSN:

2149-3189

DOI:

https://doi.org/10.18621/eurj.900847

Özet:

 


Objectives: The most diagnosed tumor among infiltrating tumors of the female genital tract is endometrial carcinoma. The Wnt/β-catenin signaling pathway has an important role in organogenesis, self-renewal of tissues, and adult stem cell maintenance. However aberrant activation of it causes many types of tumors and also related to the prognosis of patients. Therefore, we aimed to investigate whether Wnt/β-catenin pathway inhibitors have any effect on the proliferation and migration of tumor cells. 

Methods: As cancer cell line, HEC-1A endometrial adenocarcinoma was used. The Wnt/β-catenin pathway inhibitors effects on proliferation and migration were demonstrated by real-time cell analysis device and wound healing model respectively. 

Results: Wnt/β-catenin pathway inhibitors FH535 (25 μM at 36th hour, p < 0.05; 50μM at 48th hour p < 0.001) and niclosamide inhibited cell proliferation (10, 25 and 50μM at 60th hours; p < 0.01, p < 0.05 and p < 0.001, respectively) whereas ICRT14 and IWP-2 did not. However only niclosamide which is also an antihelmintic drug inhibited migration of the cells in all concentrations tested (10, 25 and 50μM, p < 0.05).


Conclusions: The present study shows that the Wnt/β-catenin pathway has an substantial role in both proliferation and migration of endometrial adenocarcinoma. We suggest that the antihelmintic drug niclosamide could be further investigated for its potential therapeutic effect in endometrial adenocarcinoma.

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Makale detayları

Effect of the Wnt/β-catenin pathway inhibitors on cell proliferation and migration of HEC-1A endometrial adenocarcinoma: experimental cell culture model

Yazarlar: Yaşam ÇİRÇİRCİ | R. Nalan TİFTİK | İsmail ÜN

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Hastalıklar

Dergi: The European Research Journal 2021 Vol.7 Issue 3, pp.218-224

Belge Tipi: Makale

Yayın Tarihi: 5/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000661928

Sıklık: İki Aylık

eISSN: 2149-3189

DOI: https://doi.org/10.18621/eurj.900847

Sayı

Yazarlar:

Yaşam ÇİRÇİRCİ | R. Nalan TİFTİK | İsmail ÜN

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Hastalıklar

Dergi:

The European Research Journal 2021 Vol.7 Issue 3, pp.218-224

Belge Tipi:

Makale

Yayın Tarihi:

5/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000661928

Sıklık:

İki Aylık

eISSN:

2149-3189

DOI:

https://doi.org/10.18621/eurj.900847