Targeting human telomeric DNA with azacyanines

Ayça Küçükakdağ DOĞU; Özgül Persil ÇETİNKOL

Makale detayları

Targeting human telomeric DNA with azacyanines

Yazarlar:

Ayça Küçükakdağ DOĞU | Özgül Persil ÇETİNKOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 4, pp.1040-1051

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725620

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Small molecules targeting telomeric DNA or its   interactions with telomerase have been an active area of cancer research. In   the present study, we investigated the interactions of six benzimidazole   compounds, called azacyanines, differing from each other in alkyl chain   length and branching in the benzimidazole ring (azamethyl, azaethyl,   azapropyl, azaisopropyl, azabutyl, and azaisobutyl) with human telomeric DNA   (tel24) in 1:1 and 1:6 ratio (tel24:azacyanine) using UV-Vis, circular   dichroism (CD), and fluorescence spectroscopy. All the compounds were   binding to tel24 as indicated by the formation of the weak induced CD band   between 320 nm and 360 nm. A substantial red shift and hypochromic effect were   observed in the UV-Vis spectrum of azamethyl or azabutyl upon binding to   tel24. No red shift or hypochromic effect was observed in the spectrum of   azaisopropyl. The denaturation temperature (Tm)m)_{m}) of tel24 increased the   most, from 65~∘∘^{\circ}C to 78 ∘∘^{\circ}C, in the presence of azabutyl in   1:6 ratio. Azaisopropyl stabilized tel24 the least under the same   conditions. The highest (7.84 ××\times  105 ±5 ±^{5} ~ \pm  3.44 ××\times    1044^{4} M−1)−1)^{-1}) and the lowest (2.04 ××\times  105±5±^{5\, }\pm  5.38   ××\times  1044^{4} M−1)−1)^{-1}) association constants were obtained for   azabutyl and azaisopropyl, respectively, by fluorescence spectroscopy.   Overall, the benzimidazole scaffold, the one-pot synthesis, and the   stabilization ability of azacyanines to tel24 make them plausible drug   candidate molecules in targeting G-quadruplexes.

Makale detayları

Targeting human telomeric DNA with azacyanines

Yazarlar: Ayça Küçükakdağ DOĞU | Özgül Persil ÇETİNKOL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 4, pp.1040-1051

Belge Tipi: Makale

Yayın Tarihi: 7/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000725620

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Ayça Küçükakdağ DOĞU | Özgül Persil ÇETİNKOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 4, pp.1040-1051

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725620

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527