Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Khalid Rasooly; Songül Yalçın Ateş; Burak Özgören; Ali Ferhan Morca; Ali Çelik

Makale detayları

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Yazarlar:

Khalid Rasooly | Songül Yalçın Ateş | Burak Özgören | Ali Ferhan Morca | Ali Çelik

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Virology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.565-573

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355254

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 Plum pox virus Potyvirus plumpoxi-PPV, one of the most significant diseases affecting stone fruit trees, has been extensively studied worldwide since its initial detection in 1917, with various diagnostic approaches explored. This study investigates the performance of colorimetric and real-time diagnostic kits developed by DOSA Information and Communication Technologies Ltd. for detecting PPV under isothermal conditions. These isothermal diagnostic kits, developed and optimized with domestic resources, stand out as highly practical diagnostic tools, leveraging an advantage of containing proprietary primer sets that enable direct testing of RNA from infected plants, independent of existing isothermal methods. In this study, the isothermal diagnostic kits enabled successful amplification of PPV at 65C within 60 minutes, without cross-reaction with potential host genomes or other viruses that could co-infect. The colorimetric and real-time diagnostic kits demonstrated detection sensitivities of 10-4 and 10-5 ngµL of PPV RNA, respectively, meeting the desired standards for PPV detection. These kits also facilitated effective amplification of PPV-M, D, and T strains, commonly found in Türkiye. Colorimetric and real-time isothermal diagnostic kits can be utilized as rapid and cost-effective tools in PPV screening programs. The application of these kits, especially in field settings and areas with limited laboratory infrastructure, should be encouraged. This successfully developed and optimized isothermal diagnostic platform could also be adapted for the detection of other significant plant viruses, offering a strategic advantage in managing viral diseases in agricultural regions such as Türkiye.

 Plum pox virus Potyvirus plumpoxi-PPV, one of the most significant diseases affecting stone fruit trees, has been extensively studied worldwide since its initial detection in 1917, with various diagnostic approaches explored. This study investigates the performance of colorimetric and real-time diagnostic kits developed by DOSA Information and Communication Technologies Ltd. for detecting PPV under isothermal conditions. These isothermal diagnostic kits, developed and optimized with domestic resources, stand out as highly practical diagnostic tools, leveraging an advantage of containing proprietary primer sets that enable direct testing of RNA from infected plants, independent of existing isothermal methods. In this study, the isothermal diagnostic kits enabled successful amplification of PPV at 65C within 60 minutes, without cross-reaction with potential host genomes or other viruses that could co-infect. The colorimetric and real-time diagnostic kits demonstrated detection sensitivities of 10-4 and 10-5 ngµL of PPV RNA, respectively, meeting the desired standards for PPV detection. These kits also facilitated effective amplification of PPV-M, D, and T strains, commonly found in Türkiye. Colorimetric and real-time isothermal diagnostic kits can be utilized as rapid and cost-effective tools in PPV screening programs. The application of these kits, especially in field settings and areas with limited laboratory infrastructure, should be encouraged. This successfully developed and optimized isothermal diagnostic platform could also be adapted for the detection of other significant plant viruses, offering a strategic advantage in managing viral diseases in agricultural regions such as Türkiye.

 Plum pox virus Potyvirus plumpoxi-PPV, one of the most significant diseases affecting stone fruit trees, has been extensively studied worldwide since its initial detection in 1917, with various diagnostic approaches explored. This study investigates the performance of colorimetric and real-time diagnostic kits developed by DOSA Information and Communication Technologies Ltd. for detecting PPV under isothermal conditions. These isothermal diagnostic kits, developed and optimized with domestic resources, stand out as highly practical diagnostic tools, leveraging an advantage of containing proprietary primer sets that enable direct testing of RNA from infected plants, independent of existing isothermal methods. In this study, the isothermal diagnostic kits enabled successful amplification of PPV at 65C within 60 minutes, without cross-reaction with potential host genomes or other viruses that could co-infect. The colorimetric and real-time diagnostic kits demonstrated detection sensitivities of 10-4 and 10-5 ngµL of PPV RNA, respectively, meeting the desired standards for PPV detection. These kits also facilitated effective amplification of PPV-M, D, and T strains, commonly found in Türkiye. Colorimetric and real-time isothermal diagnostic kits can be utilized as rapid and cost-effective tools in PPV screening programs. The application of these kits, especially in field settings and areas with limited laboratory infrastructure, should be encouraged. This successfully developed and optimized isothermal diagnostic platform could also be adapted for the detection of other significant plant viruses, offering a strategic advantage in managing viral diseases in agricultural regions such as Türkiye.

 Plum pox virus Potyvirus plumpoxi-PPV, one of the most significant diseases affecting stone fruit trees, has been extensively studied worldwide since its initial detection in 1917, with various diagnostic approaches explored. This study investigates the performance of colorimetric and real-time diagnostic kits developed by DOSA Information and Communication Technologies Ltd. for detecting PPV under isothermal conditions. These isothermal diagnostic kits, developed and optimized with domestic resources, stand out as highly practical diagnostic tools, leveraging an advantage of containing proprietary primer sets that enable direct testing of RNA from infected plants, independent of existing isothermal methods. In this study, the isothermal diagnostic kits enabled successful amplification of PPV at 65C within 60 minutes, without cross-reaction with potential host genomes or other viruses that could co-infect. The colorimetric and real-time diagnostic kits demonstrated detection sensitivities of 10-4 and 10-5 ngµL of PPV RNA, respectively, meeting the desired standards for PPV detection. These kits also facilitated effective amplification of PPV-M, D, and T strains, commonly found in Türkiye. Colorimetric and real-time isothermal diagnostic kits can be utilized as rapid and cost-effective tools in PPV screening programs. The application of these kits, especially in field settings and areas with limited laboratory infrastructure, should be encouraged. This successfully developed and optimized isothermal diagnostic platform could also be adapted for the detection of other significant plant viruses, offering a strategic advantage in managing viral diseases in agricultural regions such as Türkiye.

Makale detayları

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Yazarlar:

Khalid Rasooly | Songül Yalçın Ateş | Burak Özgören | Ali Ferhan Morca | Ali Çelik

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Virology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.565-573

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355254

pISSN:

2148-3647

PPV

Sharka Disease

Isotermal

LAMP

Kit

Sayı

Makale detayları

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Yazarlar: Khalid Rasooly | Songül Yalçın Ateş | Burak Özgören | Ali Ferhan Morca | Ali Çelik

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Plant Virology in Agriculture

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.565-573

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001355254

pISSN: 2148-3647

PPV

Sharka Disease

Isotermal

LAMP

Kit

Sayı

Yazarlar:

Khalid Rasooly | Songül Yalçın Ateş | Burak Özgören | Ali Ferhan Morca | Ali Çelik

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Virology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 3, pp.565-573

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001355254

pISSN:

2148-3647