TÜMÖR HÜCRELERİ APOPTOZ FAKTÖRÜ (TCApF)’NÜN İNSAN PROSTAT VE MEME KANSERİ HÜCRE HATLARI ÜZERİNE SİTOTOKSİK VE GENOTOKSİK ETKİLERİNİN BELİRLENMESİ

Yavuz ERDEN; Sevilay GÜNAY

Makale detayları

TÜMÖR HÜCRELERİ APOPTOZ FAKTÖRÜ (TCApF)’NÜN İNSAN PROSTAT VE MEME KANSERİ HÜCRE HATLARI ÜZERİNE SİTOTOKSİK VE GENOTOKSİK ETKİLERİNİN BELİRLENMESİ

Yazarlar:

Yavuz ERDEN | Sevilay GÜNAY

Yayıncı:

Inonu University

Ana Başlıklar:

Hastalıklar

Dergi:

Journal of Vocational School of Health Service 2020 Vol.8 Issue 2, pp.356-366

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000720589

Sıklık:

Üç Yıllık

eISSN:

2147-7892

pISSN:

2147-7892

Özet:

 


Anticancer peptides (ACP) are thought as an important strategy for molecular targeted cancer drug discovery and development process. It is predicted that new therapeutic drugs with reduced toxic effects to normal cells can be designed by using ACPs. Tumor cells apoptosis factor (TCApF) is a new peptide structured hormone with a length of 84 amino acids. Few studies on this hormone report that TCApF may be a potential ACP. The aim of this study is to determine the possible cytotoxic and genotoxic effects of TCApF on human breast (MCF-7) and prostate cancer (PC-3) cell lines. In the present study, concentrations of 1, 10 and 100 and 1000 ng/ml of TCApF, as well as a reference drug (5- fluorouracil), were applied on human breast and prostate cancer cell lines for 24 and 48 hours. Following the application, the effects of TCApF on cell viability were determined by MTT method, and the effect on DNA damage was determined by single cell gel electrophoresis method (Comet Assay). As the result, we determined that the applied dose of 1000 ng/ml reduces cell viability in both cell lines and causes low level of DNA damage. These results show that TCApF may be a potential ACP, but it does not exhibit effect at low doses.

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Makale detayları

TÜMÖR HÜCRELERİ APOPTOZ FAKTÖRÜ (TCApF)’NÜN İNSAN PROSTAT VE MEME KANSERİ HÜCRE HATLARI ÜZERİNE SİTOTOKSİK VE GENOTOKSİK ETKİLERİNİN BELİRLENMESİ

Yazarlar: Yavuz ERDEN | Sevilay GÜNAY

Yayıncı: Inonu University

Ana Başlıklar: Hastalıklar

Dergi: Journal of Vocational School of Health Service 2020 Vol.8 Issue 2, pp.356-366

Belge Tipi: Makale

Yayın Tarihi: 6/2020

Belge Dili: Türkçe

Document ID (Org No.): 2000720589

Sıklık: Üç Yıllık

eISSN: 2147-7892

pISSN: 2147-7892

Sayı

Yazarlar:

Yavuz ERDEN | Sevilay GÜNAY

Yayıncı:

Inonu University

Ana Başlıklar:

Hastalıklar

Dergi:

Journal of Vocational School of Health Service 2020 Vol.8 Issue 2, pp.356-366

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

Türkçe

Document ID (Org No.):

2000720589

Sıklık:

Üç Yıllık

eISSN:

2147-7892

pISSN:

2147-7892